• 3958 Citations
  • 36 h-Index
1982 …2021
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Research Output 1982 2019

44 Citations (Scopus)

Active site similarities of glucose dehydrogenase, glucose oxidase, and glucoamylase probed by deoxygenated substrates

Sierks, M., Bock, K., Refn, S. & Svensson, B., 1992, In : Biochemistry. 31, 37, p. 8972-8977 6 p.

Research output: Contribution to journalArticle

Glucose 1-Dehydrogenase
Glucan 1,4-alpha-Glucosidase
Glucose Oxidase
Catalytic Domain
Substrates
36 Citations (Scopus)

Activity and thermal stability of genetically truncated forms of Aspergillus glucoamylase

Evans, R., Ford, C., Sierks, M., Nikolov, Z. & Svensson, B., 1990, In : Gene. 91, 1, p. 131-134 4 p.

Research output: Contribution to journalArticle

Glucan 1,4-alpha-Glucosidase
Aspergillus
Starch
Hot Temperature
Enzymes
16 Citations (Scopus)

Aglycon mimicking: Glycoside bond cleavage transition state mimics based on hydroxypyrrolidine inhibitors

Mikkelsen, G., Christensen, T. V., Bols, M., Lundt, I. & Sierks, M., Sep 4 1995, In : Tetrahedron Letters. 36, 36, p. 6541-6544 4 p.

Research output: Contribution to journalArticle

Glycosides
96 Citations (Scopus)

A human single-chain Fv intrabody blocks aberrant cellular effects of overexpressed α-synuclein

Zhou, C., Emadi, S., Sierks, M. & Messer, A., Dec 2004, In : Molecular Therapy. 10, 6, p. 1023-1031 9 p.

Research output: Contribution to journalArticle

Synucleins
Single-Chain Antibodies
Antibodies
Detergents
Pathology
129 Citations (Scopus)

A novel nicotinic acetylcholine receptor subtype in basal forebrain cholinergic neurons with high sensitivity to amyloid peptides

Liu, Q., Huang, Y., Xue, F., Simard, A., DeChon, J., Li, G., Zhang, J., Lucero, L., Wang, M., Sierks, M., Hu, G., Chang, Y., Lukas, R. J. & Wu, J., Jan 28 2009, In : Journal of Neuroscience. 29, 4, p. 918-929 12 p.

Research output: Contribution to journalArticle

Cholinergic Neurons
Nicotinic Receptors
Amyloid
Peptides
Alzheimer Disease
22 Citations (Scopus)

A novel nicotinic mechanism underlies β-amyloid-induced neurotoxicity

Liu, Q., Xie, X., Emadi, S., Sierks, M. & Wu, J., Oct 1 2015, In : Neuropharmacology. 97, p. 457-463 7 p., 5834.

Research output: Contribution to journalArticle

Amyloid
L-Lactate Dehydrogenase
Neurons
Mecamylamine
Cholinergic Neurons
Poisons
Amyloid
Immunization
Antibodies
Neurodegenerative Diseases
Immunoglobulin Fragments
DNA-Binding Proteins
Neurodegenerative Diseases
Single-Domain Antibodies
Proteins
2 Citations (Scopus)

Antifibrillizing agents catalyze the formation of unstable intermediate aggregates of beta-amyloid

Wang, M. S., Boddapati, S. & Sierks, M., Jul 2010, In : Biotechnology Progress. 26, 4, p. 1172-1179 8 p.

Research output: Contribution to journalArticle

Amyloid
Alzheimer Disease
Poisons
Raffinose
Mannose
59 Citations (Scopus)

Anti-oligomeric Aβ Single-chain Variable Domain Antibody Blocks Aβ-induced Toxicity Against Human Neuroblastoma Cells

Zameer, A., Kasturirangan, S., Emadi, S., Nimmagadda, S. V. & Sierks, M., Dec 26 2008, In : Journal of Molecular Biology. 384, 4, p. 917-928 12 p.

Research output: Contribution to journalArticle

Neuroblastoma
Alzheimer Disease
Poisons
Bacteriophages
Antibodies
12 Citations (Scopus)

Anti-oligomeric single chain variable domain antibody differentially affects huntingtin and α-synuclein aggregates

Nannenga, B., Zameer, A. & Sierks, M., Feb 20 2008, In : FEBS Letters. 582, 4, p. 517-522 6 p.

Research output: Contribution to journalArticle

Synucleins
Agglomeration
Antibodies
Poisons
Amyloidogenic Proteins
20 Citations (Scopus)

APP/Aβ structural diversity and Alzheimer's disease pathogenesis

Roher, A. E., Kokjohn, T. A., Clarke, S. G., Sierks, M., Maarouf, C. L., Serrano, G. E., Sabbagh, M. S. & Beach, T. G., Nov 1 2017, In : Neurochemistry International. 110, p. 1-13 13 p.

Research output: Contribution to journalReview article

Alzheimer Disease
Amyloid
Post Translational Protein Processing
Dementia
Peptides
1 Citation (Scopus)
Glucosidases
septic shock
Carboxymethylcellulose Sodium
glucosidases
carboxymethylcellulose
13 Citations (Scopus)

Artificial antibodies for affinity chromatography of homologous proteins: Application to blood clotting proteins

Wu, H., Goud, G. N. & Sierks, M., May 1998, In : Biotechnology Progress. 14, 3, p. 496-499 4 p.

Research output: Contribution to journalArticle

blood coagulation
Antibody Affinity
Blood Coagulation
affinity chromatography
Affinity Chromatography
10 Citations (Scopus)

A Sensitive phage-based capture ELISA for sub-femtomolar detection of protein variants directly from biological samples

Williams, S., Schulz, P. & Sierks, M., Jan 1 2015, In : Biotechnology Progress. 31, 1, p. 289-298 10 p.

Research output: Contribution to journalArticle

Bacteriophages
Enzyme-Linked Immunosorbent Assay
Proteins
Epitopes
Capsid Proteins
1 Citation (Scopus)
Bispecific Antibodies
Amyloid Precursor Protein Secretases
Immunoglobulin Fragments
Amyloid beta-Protein Precursor
Health
Single-Domain Antibodies
Amyloid Precursor Protein Secretases
Alzheimer Disease
Immunoglobulin Fragments
Amyloid beta-Protein Precursor
11 Citations (Scopus)

Bispecific tandem single chain antibody simultaneously inhibits β-secretase and promotes α-secretase processing of AβPP

Boddapati, S., Levites, Y., Suryadi, V., Kasturirangan, S. & Sierks, M., 2012, In : Journal of Alzheimer's Disease. 28, 4, p. 961-969 9 p.

Research output: Contribution to journalArticle

Single-Chain Antibodies
Amyloid Precursor Protein Secretases
Amyloid beta-Protein Precursor
Proteolysis
Peptide Hydrolases
9 Citations (Scopus)

Blood-Based Oligomeric and Other Protein Variant Biomarkers to Facilitate Pre-Symptomatic Diagnosis and Staging of Alzheimer's Disease

Williams, S. M., Schulz, P., Rosenberry, T. L., Caselli, R. J. & Sierks, M., 2017, In : Journal of Alzheimer's Disease. 58, 1, p. 23-35 13 p.

Research output: Contribution to journalArticle

Alzheimer Disease
Biomarkers
Proteins
Single-Chain Antibodies
Immunoglobulin Fragments
114 Citations (Scopus)

Catalytic mechanism of fungal glucoamylase as defined by mutagenesis of asp176, glu179 and glu180 in the enzyme from Aspergillus awamori

Sierks, M., Ford, C., Reilly, P. J. & Svensson, B., Jan 1990, In : Protein Engineering, Design and Selection. 3, 3, p. 193-198 6 p.

Research output: Contribution to journalArticle

Maltose
Glucan 1,4-alpha-Glucosidase
Mutagenesis
Aspergillus
Hydrolysis
20 Citations (Scopus)
Glucan 1,4-alpha-Glucosidase
Mutagenesis
Fluorides
Hydrolysis
Maltose
10 Citations (Scopus)
Single-Chain Antibodies
Atomic Force Microscopy
Insulin
Amyloid
Antibodies
17 Citations (Scopus)

Characterizing antibody specificity to different protein morphologies by AFM

Wang, M. S., Zameer, A., Emadi, S. & Sierks, M., Jan 20 2009, In : Langmuir. 25, 2, p. 912-918 7 p.

Research output: Contribution to journalArticle

antibodies
Antibodies
Atomic force microscopy
atomic force microscopy
proteins

CNS disease-related protein variants as blood-based biomarkers in traumatic brain injury

Williams, S. M., Peltz, C., Yaffe, K., Schulz, P. & Sierks, M., Oct 9 2018, In : Neurology. 91, 15, p. 702-709 8 p.

Research output: Contribution to journalArticle

Central Nervous System Diseases
Biomarkers
Proteins
Neurodegenerative Diseases
Traumatic Brain Injury
189 Citations (Scopus)

Comparison of the domain-level organization of starch hydrolases and related enzymes

Jespersen, H. M., MacGregor, E. A., Sierks, M. & Svensson, B., 1991, In : Biochemical Journal. 280, 1, p. 51-55 5 p.

Research output: Contribution to journalArticle

Hydrolases
Starch
Enzymes
Cluster analysis
Amylases
48 Citations (Scopus)

Conformational targeting of fibrillar polyglutamine proteins in live cells escalates aggregation and cytotoxicity

Kvam, E., Nannenga, B., Wang, M. S., Jia, Z., Sierks, M. & Messer, A., May 28 2009, In : PLoS One. 4, 5, e5727.

Research output: Contribution to journalArticle

Cell Aggregation
Cytotoxicity
Conformations
cytotoxicity
amyloid
55 Citations (Scopus)

CSF levels of oligomeric alpha-synuclein and beta-amyloid as biomarkers for neurodegenerative disease

Sierks, M., Chatterjee, G., McGraw, C., Kasturirangan, S., Schulz, P. & Prasad, S., Dec 2011, In : Integrative Biology. 3, 12, p. 1188-1196 9 p.

Research output: Contribution to journalArticle

Neurodegenerative diseases
alpha-Synuclein
Biomarkers
Amyloid
Neurodegenerative Diseases
112 Citations (Scopus)

Curcumin reduces α-synuclein induced cytotoxicity in Parkinson's disease cell model

Wang, M. S., Boddapati, S., Emadi, S. & Sierks, M., Apr 30 2010, In : BMC Neuroscience. 11, 57.

Research output: Contribution to journalArticle

Synucleins
Curcumin
Parkinson Disease
Reactive Oxygen Species
Apoptosis
25 Citations (Scopus)

Cyclodextrins promote protein aggregation posing risks for therapeutic applications

Wang, M. S., Boddapati, S. & Sierks, M., Aug 28 2009, In : Biochemical and Biophysical Research Communications. 386, 3, p. 526-531 6 p.

Research output: Contribution to journalArticle

Cyclodextrins
Agglomeration
Type C Niemann-Pick Disease
Proteins
Neurofibrillary Tangles
58 Citations (Scopus)

Degradation of β-Amyloid by Proteolytic Antibody Light Chains

Rangan, S. K., Liu, R., Brune, D., Planque, S., Paul, S. & Sierks, M., Dec 9 2003, In : Biochemistry. 42, 48, p. 14328-14334 7 p.

Research output: Contribution to journalArticle

Amyloid
Amyloid Precursor Protein Secretases
Light
Degradation
Immunoglobulin Fragments
63 Citations (Scopus)

Detecting morphologically distinct oligomeric forms of α-synuclein

Emadi, S., Kasturirangan, S., Wang, M. S., Schulz, P. & Sierks, M., Apr 24 2009, In : Journal of Biological Chemistry. 284, 17, p. 11048-11058 11 p.

Research output: Contribution to journalArticle

Synucleins
Parkinson Disease
Agglomeration
Single-Chain Antibodies
Immunoglobulin Fragments
85 Citations (Scopus)

Ectoine and hydroxyectoine inhibit aggregation and neurotoxicity of Alzheimer's β-amyloid

Kanapathipillai, M., Lentzen, G., Sierks, M. & Park, C. B., Aug 29 2005, In : FEBS Letters. 579, 21, p. 4775-4780 6 p.

Research output: Contribution to journalArticle

Amyloid
Agglomeration
Neurodegenerative diseases
Protein Denaturation
Denaturation
14 Citations (Scopus)

Energetic and mechanistic studies of glucoamylase using molecular recognition of maltose OH groups coupled with site-directed mutagenesis

Sierks, M. & Svensson, B., Jul 25 2000, In : Biochemistry. 39, 29, p. 8585-8592 8 p.

Research output: Contribution to journalArticle

Glucan 1,4-alpha-Glucosidase
Molecular recognition
Mutagenesis
Maltose
Site-Directed Mutagenesis
13 Citations (Scopus)

Engineered proteolytic nanobodies reduce Aβ burden and ameliorate Aβ-induced cytotoxicity

Kasturirangan, S., Boddapati, S. & Sierks, M., Jun 1 2010, In : Biochemistry. 49, 21, p. 4501-4508 8 p.

Research output: Contribution to journalArticle

Single-Domain Antibodies
Cytotoxicity
Serum Amyloid A Protein
Amyloid Precursor Protein Secretases
Amyloid beta-Protein Precursor
53 Citations (Scopus)

ESCRT-mediated uptake and degradation of brain-targeted α-synuclein single chain antibody attenuates neuronal degeneration in vivo

Spencer, B., Emadi, S., Desplats, P., Eleuteri, S., Michael, S., Kosberg, K., Shen, J., Rockenstein, E., Patrick, C., Adame, A., Gonzalez, T., Sierks, M. & Masliah, E., Jan 1 2014, In : Molecular Therapy. 22, 10, p. 1753-1767 15 p.

Research output: Contribution to journalArticle

Endosomal Sorting Complexes Required for Transport
Synucleins
Single-Chain Antibodies
Apolipoproteins B
Parkinson Disease
107 Citations (Scopus)

Evaluation of isofagomine and its derivatives as potent glycosidase inhibitors

Dong, W., Jespersen, T., Bols, M., Skrydstrup, T. & Sierks, M., Feb 27 1996, In : Biochemistry. 35, 8, p. 2788-2795 8 p.

Research output: Contribution to journalArticle

Glucosidases
Glycoside Hydrolases
Glucan 1,4-alpha-Glucosidase
Oligo-1,6-Glucosidase
Derivatives
Butenes
Acetone
Fermentation
Enzyme inhibition
Butyric acid
14 Citations (Scopus)

Functional and structural roles of the highly conserved Trp120 loop region of glucoamylase from Aspergillus awamori

Natarajan, S. & Sierks, M., Mar 5 1996, In : Biochemistry. 35, 9, p. 3050-3058 9 p.

Research output: Contribution to journalArticle

Glucan 1,4-alpha-Glucosidase
Aspergillus
Substrates
Mutation
Hydrolysis
31 Citations (Scopus)
Glucan 1,4-alpha-Glucosidase
Mutagenesis
Aspergillus
Site-Directed Mutagenesis
Mutation
43 Citations (Scopus)
Glucan 1,4-alpha-Glucosidase
Mutagenesis
Aspergillus
Aspartic Acid
Tyrosine
10 Citations (Scopus)

Human α4β2 Nicotinic Acetylcholine Receptor as a Novel Target of Oligomeric α-Synuclein

Liu, Q., Emadi, S., Shen, J. X., Sierks, M. & Wu, J., Feb 20 2013, In : PLoS One. 8, 2, e55886.

Research output: Contribution to journalArticle

Synucleins
cholinergic receptors
Parkinson disease
Nicotinic Receptors
cholinergic agents

HYDROLYSIS OF CELLULOSICS BY ENTEROBACTERIA.

Sierks, M., 1983, Proceedings of the Annual Biochemical Engineering Symposium. Reilly, P. J. (ed.). Ames, IA, USA: Iowa State Univ, p. 31-38 8 p.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Hydrolysis
Cellulose
Glucosidases
8 Citations (Scopus)
Terminator Codon
stop codon
bacteriophages
Bacteriophages
Libraries
14 Citations (Scopus)

Identification of enzyme-substrate and enzyme-product complexes in the catalytic mechanism of glucoamylase from Aspergillus awamori

Natarajan, S. K. & Sierks, M., 1996, In : Biochemistry. 35, 48, p. 15269-15279 11 p.

Research output: Contribution to journalArticle

Glucan 1,4-alpha-Glucosidase
Aspergillus
Substrates
Enzymes
Fluorescence

Immunization with abeta oligomers

Sierks, M., Jun 7 2010

Research output: Patent

Immunization
Alzheimer Disease
Vaccination
Therapeutics
Brain
10 Citations (Scopus)

Improved affinity selection using phage display technology and off-rate based selection

Yuan, B., Schulz, P., Liu, R. & Sierks, M., Apr 15 2006, In : Electronic Journal of Biotechnology. 9, 2, p. 171-175 5 p.

Research output: Contribution to journalArticle

Immunoglobulin Fragments
Bacteriophages
Antibodies
Libraries
Display devices
88 Citations (Scopus)

Inhibiting Aggregation of α-Synuclein with Human Single Chain Antibody Fragments

Emadi, S., Liu, R., Yuan, B., Schulz, P., McAllister, C., Lyubchenko, Y., Messer, A. & Sierks, M., Mar 16 2004, In : Biochemistry. 43, 10, p. 2871-2878 8 p.

Research output: Contribution to journalArticle

Synucleins
Single-Chain Antibodies
Immunoglobulin Fragments
Agglomeration
Parkinson Disease
33 Citations (Scopus)

Inhibiting β-secretase activity in Alzheimer's disease cell models with single-chain antibodies specifically targeting APP

Boddapati, S., Levites, Y. & Sierks, M., Jan 14 2011, In : Journal of Molecular Biology. 405, 2, p. 436-447 12 p.

Research output: Contribution to journalArticle

Single-Chain Antibodies
Amyloid Precursor Protein Secretases
Amyloid beta-Protein Precursor
Alzheimer Disease
Amyloid
89 Citations (Scopus)

Insights into the mechanisms of action of anti-Abeta antibodies in Alzheimer's disease mouse models.

Levites, Y., Smithson, L. A., Price, R. W., Dakin, R. S., Yuan, B., Sierks, M., Kim, J., McGowan, E., Dana, K. R., Rosenberry, T. L., Das, P. & Golde, T. E., Dec 2006, In : The FASEB journal : official publication of the Federation of American Societies for Experimental Biology. 20, 14, p. 2576-2578 3 p.

Research output: Contribution to journalArticle

Anti-Idiotypic Antibodies
Alzheimer Disease
Antibodies
Brain
Life Support Care
23 Citations (Scopus)

Intracellular targeting and clearance of oligomeric alpha-synuclein alleviates toxicity in mammalian cells

Yuan, B. & Sierks, M., Jul 31 2009, In : Neuroscience Letters. 459, 1, p. 16-18 3 p.

Research output: Contribution to journalArticle

alpha-Synuclein
Immunoglobulin Fragments
Proteostasis Deficiencies
Single-Chain Antibodies
Poisons