• 3958 Citations
  • 36 h-Index
1982 …2021
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Research Output 1982 2019

1982
Butenes
Acetone
Fermentation
Enzyme inhibition
Butyric acid
1983

HYDROLYSIS OF CELLULOSICS BY ENTEROBACTERIA.

Sierks, M., 1983, Proceedings of the Annual Biochemical Engineering Symposium. Reilly, P. J. (ed.). Ames, IA, USA: Iowa State Univ, p. 31-38 8 p.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Hydrolysis
Cellulose
Glucosidases
1985
1 Citation (Scopus)
Glucosidases
septic shock
Carboxymethylcellulose Sodium
glucosidases
carboxymethylcellulose
1989
171 Citations (Scopus)

Sequence homology between putative raw-starch binding domains from different starch-degrading enzymes

Svensson, B., Jespersen, H., Sierks, M. & MacGregor, E. A., 1989, In : Biochemical Journal. 264, 1, p. 309-311 3 p.

Research output: Contribution to journalArticle

Sequence Homology
Starch
Enzymes
75 Citations (Scopus)

Site-directed mutagenesis at the active site Trpl20 of Aspergillus awamori glucoamylase

Sierks, M., Ford, C., Reilly, P. J. & Svensson, B., Aug 1989, In : Protein Engineering, Design and Selection. 2, 8, p. 621-625 5 p.

Research output: Contribution to journalArticle

Maltose
Glucan 1,4-alpha-Glucosidase
Mutagenesis
Aspergillus
Site-Directed Mutagenesis
1990
36 Citations (Scopus)

Activity and thermal stability of genetically truncated forms of Aspergillus glucoamylase

Evans, R., Ford, C., Sierks, M., Nikolov, Z. & Svensson, B., 1990, In : Gene. 91, 1, p. 131-134 4 p.

Research output: Contribution to journalArticle

Glucan 1,4-alpha-Glucosidase
Aspergillus
Starch
Hot Temperature
Enzymes
114 Citations (Scopus)

Catalytic mechanism of fungal glucoamylase as defined by mutagenesis of asp176, glu179 and glu180 in the enzyme from Aspergillus awamori

Sierks, M., Ford, C., Reilly, P. J. & Svensson, B., Jan 1990, In : Protein Engineering, Design and Selection. 3, 3, p. 193-198 6 p.

Research output: Contribution to journalArticle

Maltose
Glucan 1,4-alpha-Glucosidase
Mutagenesis
Aspergillus
Hydrolysis
1991
189 Citations (Scopus)

Comparison of the domain-level organization of starch hydrolases and related enzymes

Jespersen, H. M., MacGregor, E. A., Sierks, M. & Svensson, B., 1991, In : Biochemical Journal. 280, 1, p. 51-55 5 p.

Research output: Contribution to journalArticle

Hydrolases
Starch
Enzymes
Cluster analysis
Amylases
1992
44 Citations (Scopus)

Active site similarities of glucose dehydrogenase, glucose oxidase, and glucoamylase probed by deoxygenated substrates

Sierks, M., Bock, K., Refn, S. & Svensson, B., 1992, In : Biochemistry. 31, 37, p. 8972-8977 6 p.

Research output: Contribution to journalArticle

Glucose 1-Dehydrogenase
Glucan 1,4-alpha-Glucosidase
Glucose Oxidase
Catalytic Domain
Substrates
39 Citations (Scopus)

Kinetic identification of a hydrogen bonding pair in the glucoamylase-maltose transition state complex

Sierks, M. & Svensson, B., Mar 1992, In : Protein Engineering, Design and Selection. 5, 2, p. 185-188 4 p.

Research output: Contribution to journalArticle

Maltose
Glucan 1,4-alpha-Glucosidase
Hydrogen Bonding
Hydrogen bonds
Enzymes
70 Citations (Scopus)
Acarbose
Glucan 1,4-alpha-Glucosidase
Mutagenesis
Aspergillus niger
Aspergillus
1993
31 Citations (Scopus)
Glucan 1,4-alpha-Glucosidase
Mutagenesis
Aspergillus
Site-Directed Mutagenesis
Mutation
43 Citations (Scopus)
Glucan 1,4-alpha-Glucosidase
Mutagenesis
Aspergillus
Aspartic Acid
Tyrosine
49 Citations (Scopus)

Reaction mechanisms of Trp120→Phe and wild-type glucoamylases from Aspergillus niger. Interactions with maltooligodextrins and acarbose

Olsen, K., Christensen, U., Sierks, M. & Svensson, B., 1993, In : Biochemistry. 32, 37, p. 9686-9693 8 p.

Research output: Contribution to journalArticle

Acarbose
Glucan 1,4-alpha-Glucosidase
Aspergillus niger
Aspergillus
Mutation
213 Citations (Scopus)

Starch- and glycogen-debranching and branching enzymes: Prediction of structural features of the catalytic (β/α)8-barrel domain and evolutionary relationship to other amylolytic enzymes

Jespersen, H. M., Ann MacGregor, E., Henrissat, B., Sierks, M. & Svensson, B., Dec 1993, In : Journal of Protein Chemistry. 12, 6, p. 791-805 15 p.

Research output: Contribution to journalArticle

Glycogen Debranching Enzyme System
1,4-alpha-Glucan Branching Enzyme
Starch
Enzymes
exo-1,6-alpha-glucosidase
1994
157 Citations (Scopus)

Isofagomine, a potent, new glycosidase inhibitor

Jespersen, T. M., Dong, W., Sierks, M., Skrydstrup, T., Lundt, I. & Bols, M., Sep 16 1994, In : Angewandte Chemie (International Edition in English). 33, 17, p. 1778-1779 2 p.

Research output: Contribution to journalArticle

Glycoside Hydrolases
Atoms
Glucosidases
Derivatives
Monosaccharides
44 Citations (Scopus)
Maltose
Glucan 1,4-alpha-Glucosidase
Aspergillus
Starch
Isomaltose
82 Citations (Scopus)

Site-directed mutagenesis of the catalytic base glutamic acid 400 in glucoamylase from Aspergillus niger and of tyrosine 48 and glutamine 401, both hydrogen-bonded to the γ-carboxylate group of glutamic acid 400

Frandsen, T. P., Dupont, C., Lehmbeck, J., Stoffer, B., Sierks, M., Honzatko, R. B. & Svensson, B., 1994, In : Biochemistry. 33, 46, p. 13808-13816 9 p.

Research output: Contribution to journalArticle

Glucan 1,4-alpha-Glucosidase
Mutagenesis
Aspergillus niger
Aspergillus
Site-Directed Mutagenesis
76 Citations (Scopus)

Synthesis of isofagomine, a novel glycosidase inhibitor

M. Jespersen, T., Bols, M., Sierks, M. & Skrydstrup, T., 1994, In : Tetrahedron. 50, 47, p. 13449-13460 12 p.

Research output: Contribution to journalArticle

Glycoside Hydrolases
Epoxy Compounds
Glucosidases
Amination
Lactams
1995
16 Citations (Scopus)

Aglycon mimicking: Glycoside bond cleavage transition state mimics based on hydroxypyrrolidine inhibitors

Mikkelsen, G., Christensen, T. V., Bols, M., Lundt, I. & Sierks, M., Sep 4 1995, In : Tetrahedron Letters. 36, 36, p. 6541-6544 4 p.

Research output: Contribution to journalArticle

Glycosides
22 Citations (Scopus)

Synthesis of the first pseudosugar-C-disaccharide. A potential antigen for eliciting glycoside-bond forming antibodies with catalytic groups

Barbaud, C., Bols, M., Lundt, I. & Sierks, M., Aug 14 1995, In : Tetrahedron. 51, 33, p. 9063-9078 16 p.

Research output: Contribution to journalArticle

Carbasugars
Catalytic Antibodies
Cellobiose
Azides
Disaccharides
1996
20 Citations (Scopus)
Glucan 1,4-alpha-Glucosidase
Mutagenesis
Fluorides
Hydrolysis
Maltose
107 Citations (Scopus)

Evaluation of isofagomine and its derivatives as potent glycosidase inhibitors

Dong, W., Jespersen, T., Bols, M., Skrydstrup, T. & Sierks, M., Feb 27 1996, In : Biochemistry. 35, 8, p. 2788-2795 8 p.

Research output: Contribution to journalArticle

Glucosidases
Glycoside Hydrolases
Glucan 1,4-alpha-Glucosidase
Oligo-1,6-Glucosidase
Derivatives
14 Citations (Scopus)

Functional and structural roles of the highly conserved Trp120 loop region of glucoamylase from Aspergillus awamori

Natarajan, S. & Sierks, M., Mar 5 1996, In : Biochemistry. 35, 9, p. 3050-3058 9 p.

Research output: Contribution to journalArticle

Glucan 1,4-alpha-Glucosidase
Aspergillus
Substrates
Mutation
Hydrolysis
14 Citations (Scopus)

Identification of enzyme-substrate and enzyme-product complexes in the catalytic mechanism of glucoamylase from Aspergillus awamori

Natarajan, S. K. & Sierks, M., 1996, In : Biochemistry. 35, 48, p. 15269-15279 11 p.

Research output: Contribution to journalArticle

Glucan 1,4-alpha-Glucosidase
Aspergillus
Substrates
Enzymes
Fluorescence
1997
10 Citations (Scopus)

Minimizing nonproductive substrate binding: A new look at glucoamylase subsite affinities

Natarajan, S. K. & Sierks, M., Dec 2 1997, In : Biochemistry. 36, 48, p. 14946-14955 10 p.

Research output: Contribution to journalArticle

Glucan 1,4-alpha-Glucosidase
Glucose
Maltose
Substrates
Kinetics
23 Citations (Scopus)

Solvent and viscosity effects on the rate-limiting product release step of glucoamylase during maltose hydrolysis

Sierks, M., Sico, C. & Zaw, M., Sep 1997, In : Biotechnology Progress. 13, 5, p. 601-608 8 p.

Research output: Contribution to journalArticle

Glucan 1,4-alpha-Glucosidase
glucan 1,4-alpha-glucosidase
Maltose
maltose
Viscosity
1998
13 Citations (Scopus)

Artificial antibodies for affinity chromatography of homologous proteins: Application to blood clotting proteins

Wu, H., Goud, G. N. & Sierks, M., May 1998, In : Biotechnology Progress. 14, 3, p. 496-499 4 p.

Research output: Contribution to journalArticle

blood coagulation
Antibody Affinity
Blood Coagulation
affinity chromatography
Affinity Chromatography
2000
14 Citations (Scopus)

Energetic and mechanistic studies of glucoamylase using molecular recognition of maltose OH groups coupled with site-directed mutagenesis

Sierks, M. & Svensson, B., Jul 25 2000, In : Biochemistry. 39, 29, p. 8585-8592 8 p.

Research output: Contribution to journalArticle

Glucan 1,4-alpha-Glucosidase
Molecular recognition
Mutagenesis
Maltose
Site-Directed Mutagenesis
2001
6 Citations (Scopus)

Pleomorphism of the marine bacterium Teredinobacter turnirae

Ferreira, G. M., Ahuja, S. K., Sierks, M. & Moreira, A. R., 2001, In : Letters in Applied Microbiology. 33, 1, p. 56-60 5 p.

Research output: Contribution to journalArticle

Bacteria
Cellulase
bacterium
Peptide Hydrolases
Caseins
6 Citations (Scopus)

Specific glycosidase activity isolated from a random phage display antibody library

Goud, G. N., Artsaenko, O., Bols, M. & Sierks, M., 2001, In : Biotechnology Progress. 17, 1, p. 197-202 6 p.

Research output: Contribution to journalArticle

glycosidases
Glycoside Hydrolases
bacteriophages
Bacteriophages
linkage (genetics)
2003
58 Citations (Scopus)

Degradation of β-Amyloid by Proteolytic Antibody Light Chains

Rangan, S. K., Liu, R., Brune, D., Planque, S., Paul, S. & Sierks, M., Dec 9 2003, In : Biochemistry. 42, 48, p. 14328-14334 7 p.

Research output: Contribution to journalArticle

Amyloid
Amyloid Precursor Protein Secretases
Light
Degradation
Immunoglobulin Fragments
2004
96 Citations (Scopus)

A human single-chain Fv intrabody blocks aberrant cellular effects of overexpressed α-synuclein

Zhou, C., Emadi, S., Sierks, M. & Messer, A., Dec 2004, In : Molecular Therapy. 10, 6, p. 1023-1031 9 p.

Research output: Contribution to journalArticle

Synucleins
Single-Chain Antibodies
Antibodies
Detergents
Pathology
88 Citations (Scopus)

Inhibiting Aggregation of α-Synuclein with Human Single Chain Antibody Fragments

Emadi, S., Liu, R., Yuan, B., Schulz, P., McAllister, C., Lyubchenko, Y., Messer, A. & Sierks, M., Mar 16 2004, In : Biochemistry. 43, 10, p. 2871-2878 8 p.

Research output: Contribution to journalArticle

Synucleins
Single-Chain Antibodies
Immunoglobulin Fragments
Agglomeration
Parkinson Disease
33 Citations (Scopus)

Proteolytic antibody light chains alter β-amyloid aggregation and prevent cytotoxicity

Liu, R., McAllister, C., Lyubchenko, Y. & Sierks, M., Aug 10 2004, In : Biochemistry. 43, 31, p. 9999-10007 9 p.

Research output: Contribution to journalArticle

Amyloid Precursor Protein Secretases
Cytotoxicity
Amyloid
Agglomeration
Carboxypeptidases
128 Citations (Scopus)

Residues 17-20 and 30-35 of Beta-Amyloid Play Critical Roles in Aggregation

Liu, R., McAllister, C., Lyubchenko, Y. & Sierks, M., Jan 15 2004, In : Journal of Neuroscience Research. 75, 2, p. 162-171 10 p.

Research output: Contribution to journalArticle

Poisons
Amyloid
Fluorescence
Staining and Labeling
Peptide Fragments
103 Citations (Scopus)

Single chain variable fragments against β-amyloid (Aβ) can inhibit Aβ aggregation and prevent Aβ-induced neurotoxicity

Liu, R., Yuan, B., Emadi, S., Zameer, A., Schulz, P., McAllister, C., Lyubchenko, Y., Goud, G. & Sierks, M., Jun 8 2004, In : Biochemistry. 43, 22, p. 6959-6967 9 p.

Research output: Contribution to journalArticle

Single-Chain Antibodies
Amyloid
Alzheimer Disease
Agglomeration
Antibodies
2005
85 Citations (Scopus)

Ectoine and hydroxyectoine inhibit aggregation and neurotoxicity of Alzheimer's β-amyloid

Kanapathipillai, M., Lentzen, G., Sierks, M. & Park, C. B., Aug 29 2005, In : FEBS Letters. 579, 21, p. 4775-4780 6 p.

Research output: Contribution to journalArticle

Amyloid
Agglomeration
Neurodegenerative diseases
Protein Denaturation
Denaturation
81 Citations (Scopus)

Quantification of cytokines involved in wound healing using surface plasmon resonance

Battaglia, T. M., Masson, J. F., Sierks, M., Beaudoin, S. P., Rogers, J., Foster, K. N., Holloway, G. A. & Booksh, K. S., Nov 1 2005, In : Analytical Chemistry. 77, 21, p. 7016-7023 8 p.

Research output: Contribution to journalArticle

Surface plasmon resonance
Cell culture
Culture Media
Palmitic Acid
Cytokines
228 Citations (Scopus)

Trehalose differentially inhibits aggregation and neurotoxicity of beta-amyloid 40 and 42

Liu, R., Barkhordarian, H., Emadi, S., Chan, B. P. & Sierks, M., Oct 2005, In : Neurobiology of Disease. 20, 1, p. 74-81 8 p.

Research output: Contribution to journalArticle

Trehalose
Amyloid
Alzheimer Disease
Atomic Force Microscopy
Disaccharides
2006
8 Citations (Scopus)
Terminator Codon
stop codon
bacteriophages
Bacteriophages
Libraries
10 Citations (Scopus)

Improved affinity selection using phage display technology and off-rate based selection

Yuan, B., Schulz, P., Liu, R. & Sierks, M., Apr 15 2006, In : Electronic Journal of Biotechnology. 9, 2, p. 171-175 5 p.

Research output: Contribution to journalArticle

Immunoglobulin Fragments
Bacteriophages
Antibodies
Libraries
Display devices
89 Citations (Scopus)

Insights into the mechanisms of action of anti-Abeta antibodies in Alzheimer's disease mouse models.

Levites, Y., Smithson, L. A., Price, R. W., Dakin, R. S., Yuan, B., Sierks, M., Kim, J., McGowan, E., Dana, K. R., Rosenberry, T. L., Das, P. & Golde, T. E., Dec 2006, In : The FASEB journal : official publication of the Federation of American Societies for Experimental Biology. 20, 14, p. 2576-2578 3 p.

Research output: Contribution to journalArticle

Anti-Idiotypic Antibodies
Alzheimer Disease
Antibodies
Brain
Life Support Care
41 Citations (Scopus)

Isolating recombinant antibodies against specific protein morphologies using atomic force microscopy and phage display technologies

Barkhordarian, H., Emadi, S., Schulz, P. & Sierks, M., Nov 2006, In : Protein Engineering, Design and Selection. 19, 11, p. 497-502 6 p.

Research output: Contribution to journalArticle

Synucleins
Bacteriophages
Atomic Force Microscopy
Antibodies
Antigens
18 Citations (Scopus)

Isolation of an scFv targeting BRG1 using phage display with characterization by AFM

Marcus, W. D., Wang, H., Lohr, D., Sierks, M. & Lindsay, S., Apr 21 2006, In : Biochemical and Biophysical Research Communications. 342, 4, p. 1123-1129 7 p.

Research output: Contribution to journalArticle

Bacteriophages
Genes
Display devices
Chromatin Assembly and Disassembly
Antibodies
alpha-Synuclein
Immunoglobulin Fragments
Poisons
Bacteriophages
Huntington Disease
56 Citations (Scopus)

Reduction of nonspecific protein binding on surface plasmon resonance biosensors

Masson, J. F., Battaglia, T. M., Cramer, J., Beaudoin, S., Sierks, M. & Booksh, K. S., Dec 2006, In : Analytical and Bioanalytical Chemistry. 386, 7-8, p. 1951-1959 9 p.

Research output: Contribution to journalArticle

Surface Plasmon Resonance
Surface plasmon resonance
Biosensing Techniques
Biomarkers
Protein Binding
42 Citations (Scopus)

Single chain Fv antibodies against the 25-35 Aβ fragment inhibit aggregation and toxicity of Aβ42

Zameer, A., Schulz, P., Wang, M. S. & Sierks, M., Sep 26 2006, In : Biochemistry. 45, 38, p. 11532-11539 8 p.

Research output: Contribution to journalArticle

Single-Chain Antibodies
Toxicity
Agglomeration
Alzheimer Disease
Antibodies
2007
121 Citations (Scopus)

Isolation of a Human Single Chain Antibody Fragment Against Oligomeric α-Synuclein that Inhibits Aggregation and Prevents α-Synuclein-induced Toxicity

Emadi, S., Barkhordarian, H., Wang, M. S., Schulz, P. & Sierks, M., May 11 2007, In : Journal of Molecular Biology. 368, 4, p. 1132-1144 13 p.

Research output: Contribution to journalArticle

Synucleins
Single-Chain Antibodies
Immunoglobulin Fragments
Intrinsically Disordered Proteins
Lewy Bodies
15 Citations (Scopus)

Single-molecule selection and recovery of structure-specific antibodies using atomic force microscopy

Shlyakhtenko, L. S., Yuan, B., Emadi, S., Lyubchenko, Y. L. & Sierks, M., Sep 2007, In : Nanomedicine: Nanotechnology, Biology, and Medicine. 3, 3, p. 192-197 6 p.

Research output: Contribution to journalArticle

Atomic Force Microscopy
Antibodies
Atomic force microscopy
Proteins
Recovery