Virion-Associated Nucleic Acid-Based Metagenomics: A Decade of Advances in Molecular Characterization of Plant Viruses

Oumaima Moubset, Sarah François, François Maclot, Essowè Palanga, Charlotte Julian, Lisa Claude, Emmanuel Fernandez, Philippe Rott, Jean Heinrich Daugrois, Aymeric Antoine-Lorquin, Pauline Bernardo, Arnaud G. Blouin, Coline Temple, Simona Kraberger, Rafaela S. Fontenele, Gordon W. Harkins, Yuxin Ma, Armelle Marais, Thierry Candresse, Sélim Ben ChéhidaPierre Lefeuvre, Jean Michel Lett, Arvind Varsani, Sébastien Massart, Mylène Ogliastro, Darren P. Martin, Denis Filloux, Philippe Roumagnac

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

Over the last decade, viral metagenomic studies have resulted in the discovery of thousands of previously unknown viruses. These studies are likely to play a pivotal role in obtaining an accurate and robust understanding of how viruses affect the stability and productivity of ecosystems. Among the metagenomics-based approaches that have been developed since the beginning of the 21st century, shotgun metagenomics applied specifically to virion-associated nucleic acids (VANA) has been used to disentangle the diversity of the viral world. We summarize herein the results of 24 VANA-based studies, focusing on plant and insect samples conducted over the last decade (2010 to 2020). Collectively, viruses from 85 different families were reliably detected in these studies, including capsidless RNA viruses that replicate in fungi, oomycetes, and plants. Finally, strengths and weaknesses of the VANA approach are summarized and perspectives of applications in detection, epidemiological surveillance, environmental monitoring, and ecology of plant viruses are provided. [Formula: see text]

Original languageEnglish (US)
Pages (from-to)2253-2272
Number of pages20
JournalPhytopathology
Volume112
Issue number11
DOIs
StatePublished - Nov 1 2022
Externally publishedYes

Keywords

  • ecology
  • pathogen detection
  • virology

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Plant Science

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