Ultrastructural aspects of the hyphal tip of Sclerotium rolfsii preserved by freeze substitution

R. W. Roberson, M. S. Fuller

Research output: Contribution to journalArticle

79 Scopus citations

Abstract

The hyphal tip of Sclerotium rolfsii was examined after fixation by freeze substitution. The Spitzenkörper consisted of a dense mass of apical vesicles and microvesicles surrounding a vesicle-free zone. Linear arrangements of microvesicles were occasionally observed within the Spitzenkörper. Abundant microfilaments were seen within the Spitzenkörper region, often in close association with apical vesicles and microvesicles. Microtubules passed through the Spitzenkörper and terminated at the plasmalemma at the extreme hyphal apex. Filasomes were mostly observed within the apical region and were in close proximity to the plasmalemma. Rough ER, mitochondria, microtubules, and vacuoles were abundant in the subapical region and were usually oriented parallel to the long axis of the hypha. Ribosomes were aligned on the outer surfaces of mitochondria. Golgi body equivalents were observed throughout the subapical region and appeared as inflated cisternae of varying shapes and electron opacities. Relationships to other basidiomycetous hyphal tip cells are discussed.

Original languageEnglish (US)
Pages (from-to)143-149
Number of pages7
JournalProtoplasma
Volume146
Issue number2-3
DOIs
StatePublished - Jun 1 1988
Externally publishedYes

Keywords

  • Freeze substitution
  • Hyphal tip
  • Sclerotium rolfsii
  • Ultrastructure

ASJC Scopus subject areas

  • Plant Science
  • Cell Biology

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