Tumorigenic poxviruses: Construction of the composite physical map of the Shope fibroma virus genome

A. M. Delange, C. Macaulay, W. Block, T. Mueller, Douglas McFadden

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

The sites for the restriction enzymes BamHI, BglI, HindIII, PstI, PvuII, and SstI on the linear DNA genome of Shope fibroma virus, a tumorigenic poxvirus of rabbits, have been determined by digestions of the cloned BamHI and HindIII restriction fragments and by hybridization of 32P-labeled cloned fragments to Southern blots of Shope fibroma virus DNA cleaved partially or completely with the various enzymes. The linear genome is shown to be 160 kilobases in length and to possess terminal inverted repeat sequences of between 12.2 and 12.5 kilobases extending inwards from the cross-linked DNA telomeres. The fine map of the Shope fibroma virus terminal inverted repeats has been constructed and shown to be distinctly different from that of members of the orthopoxvirus group, such as vaccinia, by the absence of detectable tandemly repeated sequences near the termini and by the lack of detectable sequence homology with vaccinia termini.

Original languageEnglish (US)
Pages (from-to)408-416
Number of pages9
JournalJournal of Virology
Volume50
Issue number2
StatePublished - Jun 15 1984
Externally publishedYes

Fingerprint

Rabbit fibroma virus
Rabbit Fibroma Virus
Poxviridae
physical chromosome mapping
Vaccinia
terminal repeat sequences
Terminal Repeat Sequences
Vaccinium
Genome
genome
DNA
Orthopoxvirus
Inverted Repeat Sequences
telomeres
Telomere
Enzymes
Sequence Homology
enzymes
Southern Blotting
sequence homology

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

Cite this

Tumorigenic poxviruses : Construction of the composite physical map of the Shope fibroma virus genome. / Delange, A. M.; Macaulay, C.; Block, W.; Mueller, T.; McFadden, Douglas.

In: Journal of Virology, Vol. 50, No. 2, 15.06.1984, p. 408-416.

Research output: Contribution to journalArticle

Delange, A. M. ; Macaulay, C. ; Block, W. ; Mueller, T. ; McFadden, Douglas. / Tumorigenic poxviruses : Construction of the composite physical map of the Shope fibroma virus genome. In: Journal of Virology. 1984 ; Vol. 50, No. 2. pp. 408-416.
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