Triangulated Wireframe Structures Assembled Using Single-Stranded DNA Tiles

Michael Matthies, Nayan P. Agarwal, Erik Poppleton, Foram M. Joshi, Petr Sulc, Thorsten L. Schmidt

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The field of structural DNA nanotechnology offers a wide range of design strategies with which to build structures with a desired aspect ratio, size, and shape. Compared with traditional close-packed DNA structures, triangulated wireframe structures require less material per surface or volume unit and improve the stability in biologically relevant conditions due to the reduced electrostatic repulsion. Herein, we expand the design space of the DNA single-stranded tile method to cover a range of anisotropic, finite, triangulated wireframe structures as well as a number of one-dimensional crystalline assemblies. These structures are composed of six-arm junctions with a single double helix as connecting edges that assemble in physiologically relevant salinities. For a reliable folding of the structures, single-stranded spacers 2-4 nucleotides long have to be introduced in the junction connecting neighboring arms. Coarse-grained molecular dynamics simulations using the oxDNA model suggests that the spacers prevent the stacking of DNA helices, thereby facilitating the assembly of planar geometries.

Original languageEnglish (US)
JournalACS Nano
DOIs
StateAccepted/In press - Jan 1 2019
Externally publishedYes

Fingerprint

Single-Stranded DNA
tiles
Tile
DNA
deoxyribonucleic acid
helices
spacers
Nanotechnology
Molecular dynamics
Aspect ratio
Electrostatics
nucleotides
Nucleotides
nanotechnology
salinity
folding
assemblies
Crystalline materials
aspect ratio
Geometry

Keywords

  • molecular dynamics simulations
  • single-stranded tiles
  • structural DNA nanotechnology
  • triangulated wireframe structures

ASJC Scopus subject areas

  • Materials Science(all)
  • Engineering(all)
  • Physics and Astronomy(all)

Cite this

Matthies, M., Agarwal, N. P., Poppleton, E., Joshi, F. M., Sulc, P., & Schmidt, T. L. (Accepted/In press). Triangulated Wireframe Structures Assembled Using Single-Stranded DNA Tiles. ACS Nano. https://doi.org/10.1021/acsnano.8b08009

Triangulated Wireframe Structures Assembled Using Single-Stranded DNA Tiles. / Matthies, Michael; Agarwal, Nayan P.; Poppleton, Erik; Joshi, Foram M.; Sulc, Petr; Schmidt, Thorsten L.

In: ACS Nano, 01.01.2019.

Research output: Contribution to journalArticle

Matthies, Michael ; Agarwal, Nayan P. ; Poppleton, Erik ; Joshi, Foram M. ; Sulc, Petr ; Schmidt, Thorsten L. / Triangulated Wireframe Structures Assembled Using Single-Stranded DNA Tiles. In: ACS Nano. 2019.
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