TY - JOUR
T1 - Transient localizations of messenger RNA in Xenopus laevis oocytes
AU - Capco, David G.
AU - Jeffery, William R.
N1 - Funding Information:
Dr. Herbert Jlckle is thanked for many critical discussions and encouragement during the course of this study. We thank Mr. Lewis Patterson for help in the preparation of the manuscript. This research was supported by grants from the National Institutes of Health (HD-13970 and GM-25119) awarded to W. R. Jeffery.
PY - 1982/1
Y1 - 1982/1
N2 - The distribution of poly(A)-containing RNA [poly(A)+ RNA] was examined in sections of oocytes, mature eggs, and early embryos of Xenopus laevis by in situ hybridization with [3H]poly(U). Poly(A)+ RNA was uniformly distributed in the cytoplasm of previtellogenic oocytes. In contrast, distinct cytoplasmic regions exhibiting high concentrations of poly(A)+ RNA were detected in vitellogenic and full-grown oocytes. These regions include a perinuclear area located between the germinal vesicle (GV) and the cortex of stage 3 oocytes, a cortical area in stage 4 oocytes, a vegetal subcortical area and a subnuclear zone in stage 5 oocytes, and a vegetal subcortical area in stage 6 oocytes. No selective accumulations of poly(A)+ RNA were observed in the mitochondrial mass of previtellogenic oocytes or in the GV at any time during oogenesis. The poly(A)+ RNA localizations in the peripheral regions of full-grown oocytes disappeared during the first maturation division leading to a uniform poly(A)+ RNA distribution in the mature egg and did not reappear during early embryogenesis. The results suggest that transient localizations of poly(A)+ RNA are present in the oocyte cytoplasm during oogenesis of Xenopus laevis.
AB - The distribution of poly(A)-containing RNA [poly(A)+ RNA] was examined in sections of oocytes, mature eggs, and early embryos of Xenopus laevis by in situ hybridization with [3H]poly(U). Poly(A)+ RNA was uniformly distributed in the cytoplasm of previtellogenic oocytes. In contrast, distinct cytoplasmic regions exhibiting high concentrations of poly(A)+ RNA were detected in vitellogenic and full-grown oocytes. These regions include a perinuclear area located between the germinal vesicle (GV) and the cortex of stage 3 oocytes, a cortical area in stage 4 oocytes, a vegetal subcortical area and a subnuclear zone in stage 5 oocytes, and a vegetal subcortical area in stage 6 oocytes. No selective accumulations of poly(A)+ RNA were observed in the mitochondrial mass of previtellogenic oocytes or in the GV at any time during oogenesis. The poly(A)+ RNA localizations in the peripheral regions of full-grown oocytes disappeared during the first maturation division leading to a uniform poly(A)+ RNA distribution in the mature egg and did not reappear during early embryogenesis. The results suggest that transient localizations of poly(A)+ RNA are present in the oocyte cytoplasm during oogenesis of Xenopus laevis.
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U2 - 10.1016/0012-1606(82)90288-3
DO - 10.1016/0012-1606(82)90288-3
M3 - Article
C2 - 6172301
AN - SCOPUS:0020085533
SN - 0012-1606
VL - 89
SP - 1
EP - 12
JO - Developmental Biology
JF - Developmental Biology
IS - 1
ER -