Transient expression of Human papillomavirus type 16 L1 protein in Nicotiana benthamiana using an infectious tobamovirus vector

Arvind Varsani, Anna Lise Williamson, Debbie Stewart, Edward P. Rybicki

Research output: Contribution to journalArticle

50 Citations (Scopus)

Abstract

A Tobacco mosaic virus (TMV)-derived vector was used to express a native Human papillomavirus type 16 (HPV-16) L1 gene in Nicotiana benthamiana by means of infectious in vitro RNA transcripts inoculated onto N. benthamiana plants. HPV-16 L1 protein expression was quantitated by enzyme-linked immunosorbent assays (ELISA) after concentration of the plant extract. We estimated that the L1 product yield was 20-37 μg/kg of fresh leaf material. The L1 protein in the concentrated extract was antigenically characterised using the neutralising and conformation-specific Mabs H16:V5 and H16:E70, which bound to the plant-produced protein. Particles observed by transmission electron microscopy were mainly capsomers but virus-like particles (VLPs) similar to those produced in other systems were also present. Immunisation of rabbits with the concentrated plant extract induced a weak immune response. This is the first report of the successful expression of an HPV L1 gene in plants using a plant virus vector.

Original languageEnglish (US)
Pages (from-to)91-96
Number of pages6
JournalVirus Research
Volume120
Issue number1-2
DOIs
StatePublished - Sep 2006
Externally publishedYes

Fingerprint

Tobamovirus
Plant Extracts
Tobacco
Tobacco Mosaic Virus
Plant Viruses
Plant Genes
Plant Proteins
Human papillomavirus 16
Transmission Electron Microscopy
Population Groups
Virion
Immunization
Enzyme-Linked Immunosorbent Assay
RNA
Rabbits
Genes
Proteins
asoxime chloride
Human papillomavirus type 16 L1 protein

Keywords

  • HPV-16
  • Papillomavirus
  • Plant expression
  • TMV vector
  • Vaccine
  • VLP

ASJC Scopus subject areas

  • Cancer Research
  • Molecular Biology
  • Virology

Cite this

Transient expression of Human papillomavirus type 16 L1 protein in Nicotiana benthamiana using an infectious tobamovirus vector. / Varsani, Arvind; Williamson, Anna Lise; Stewart, Debbie; Rybicki, Edward P.

In: Virus Research, Vol. 120, No. 1-2, 09.2006, p. 91-96.

Research output: Contribution to journalArticle

Varsani, Arvind ; Williamson, Anna Lise ; Stewart, Debbie ; Rybicki, Edward P. / Transient expression of Human papillomavirus type 16 L1 protein in Nicotiana benthamiana using an infectious tobamovirus vector. In: Virus Research. 2006 ; Vol. 120, No. 1-2. pp. 91-96.
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