Transgenic analysis of the Smad family of TGF-β signal transducers in Drosophila melanogaster suggests new roles and new interactions between family members

R. M. Marquez, M. A. Singer, N. T. Takaesu, W. R. Waldrip, Y. Kraytsberg, Stuart Newfeld

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

Smad signal transducers are required for transforming growth factor-β-mediated developmental events in many organisms including humans. However, the roles of individual human Smad genes (hSmads) in development are largely unknown. Our hypothesis is that an hSmad performs developmental roles analogous to those of the most similar Drosophila Smad gene (dSmad). We expressed six hSmad and four dSmad transgenes in Drosophila melanogaster using the Ga14/UAS system and compared their phenotypes. Phylogenetically related human and Drosophila Smads induced similar phenotypes supporting the hypothesis. In contrast, two nearly identical hSmads generated distinct phenotypes. When expressed in wing imaginal disks, hSmad2 induced oversize wings while hSmad3 induced cell death. This observation suggests that a very small number of amino acid differences, between Smads in the same species, confer distinct developmental roles. Our observations also suggest new roles for the dSmads, Med and Dad, in dActivin signaling and potential interactions between these family members. Overall, the study demonstrates that transgenic methods in Drosophila can provide new information about non-Drosophila members of developmentally important multigene families.

Original languageEnglish (US)
Pages (from-to)1639-1648
Number of pages10
JournalGenetics
Volume157
Issue number4
StatePublished - 2001

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Drosophila melanogaster
Transducers
Drosophila
Phenotype
Genes
Imaginal Discs
Transforming Growth Factors
Multigene Family
Transgenes
Cell Death
Amino Acids

ASJC Scopus subject areas

  • Genetics
  • Genetics(clinical)

Cite this

Transgenic analysis of the Smad family of TGF-β signal transducers in Drosophila melanogaster suggests new roles and new interactions between family members. / Marquez, R. M.; Singer, M. A.; Takaesu, N. T.; Waldrip, W. R.; Kraytsberg, Y.; Newfeld, Stuart.

In: Genetics, Vol. 157, No. 4, 2001, p. 1639-1648.

Research output: Contribution to journalArticle

Marquez, R. M. ; Singer, M. A. ; Takaesu, N. T. ; Waldrip, W. R. ; Kraytsberg, Y. ; Newfeld, Stuart. / Transgenic analysis of the Smad family of TGF-β signal transducers in Drosophila melanogaster suggests new roles and new interactions between family members. In: Genetics. 2001 ; Vol. 157, No. 4. pp. 1639-1648.
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AU - Kraytsberg, Y.

AU - Newfeld, Stuart

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