Transformation of chloroplasts with the psaB gene encoding a polypeptide of the photosystem I reaction center

Scott E. Bingham, Ruohui Xu, Andrew Webber

Research output: Contribution to journalArticle

23 Scopus citations

Abstract

A chloroplast photosystem I reaction center mutation. ac-u-g-2.3. of Chlamydomonas reinhardtii has been complemented with a wild type psaB gene to restore photosynthetic competence. The mutation was mapped in the psaB coding sequence by chloroplast transformation using subcloned restriction fragments of psaB. The mutation was found to be a single base pair deletion resulting in a reading frame shift and premature termination of the polypeptide. Transformants were verified by insertion of a site-directed mutation which created a new restriction enzyme site. These transformations demontrate the feasibility of insertion of site-directed mutations into the psaB gene in order to elucidate amino acid residues involved in photosystem I assembly and function.

Original languageEnglish (US)
Pages (from-to)137-140
Number of pages4
JournalFEBS Letters
Volume292
Issue number1-2
DOIs
StatePublished - Nov 4 1991

Keywords

  • Chlamydomonas reinhardtii
  • Chloroplast
  • Complementation
  • Photosystem I
  • Transformation

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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