Tracking the Antibody Immunome in Type 1 Diabetes Using Protein Arrays

Xiaofang Bian, Clive Wasserfall, Garrick Wallstrom, Jie Wang, Haoyu Wang, Kristi Barker, Desmond Schatz, Mark Atkinson, Ji Qiu, Joshua LaBaer

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

We performed an unbiased proteome-scale profiling of humoral autoimmunity in recent-onset type 1 diabetes (T1D) patients and nondiabetic controls against ∼10 000 human proteins using a Nucleic Acid Programmable Protein Array (NAPPA) platform, complemented by a knowledge-based selection of proteins from genes enriched in human pancreas. Although the global response was similar between cases and controls, we identified and then validated six specific novel T1D-associated autoantibodies (AAbs) with sensitivities that ranged from 16 to 27% at 95% specificity. These included AAbs against PTPRN2, MLH1, MTIF3, PPIL2, NUP50 (from NAPPA screening), and QRFPR (by targeted ELISA). Immunohistochemistry demonstrated that NUP50 protein behaved differently in islet cells, where it stained both nucleus and cytoplasm, compared with only nuclear staining in exocrine pancreas. Conversely, PPIL2 staining was absent in islet cells, despite its presence in exocrine cells. The combination of anti-PTPRN2, -MLH1, -PPIL2, and -QRFPR had an AUC of 0.74 and 37.5% sensitivity at 95% specificity. These data indicate that these markers behave independently and support the use of unbiased screening to find biomarkers because the majority was not predicted based on predicted abundance. Our study enriches the knowledge of the "autoantibody-ome" in unprecedented breadth and width.

Original languageEnglish (US)
Pages (from-to)195-203
Number of pages9
JournalJournal of Proteome Research
Volume16
Issue number1
DOIs
StatePublished - Jan 6 2017

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Protein Array Analysis
Medical problems
Type 1 Diabetes Mellitus
Autoantibodies
Islets of Langerhans
Nucleic Acids
Antibodies
Staining and Labeling
Exocrine Pancreas
Proteins
Proteome
Autoimmunity
Screening
Area Under Curve
Pancreas
Cytoplasm
Biomarkers
Enzyme-Linked Immunosorbent Assay
Immunohistochemistry

Keywords

  • autoantibody (AAb)
  • biomarkers
  • nucleic acid programmable protein array (NAPPA)
  • protein array
  • type 1 diabetes (T1D)

ASJC Scopus subject areas

  • Chemistry(all)
  • Biochemistry

Cite this

Tracking the Antibody Immunome in Type 1 Diabetes Using Protein Arrays. / Bian, Xiaofang; Wasserfall, Clive; Wallstrom, Garrick; Wang, Jie; Wang, Haoyu; Barker, Kristi; Schatz, Desmond; Atkinson, Mark; Qiu, Ji; LaBaer, Joshua.

In: Journal of Proteome Research, Vol. 16, No. 1, 06.01.2017, p. 195-203.

Research output: Contribution to journalArticle

Bian, X, Wasserfall, C, Wallstrom, G, Wang, J, Wang, H, Barker, K, Schatz, D, Atkinson, M, Qiu, J & LaBaer, J 2017, 'Tracking the Antibody Immunome in Type 1 Diabetes Using Protein Arrays', Journal of Proteome Research, vol. 16, no. 1, pp. 195-203. https://doi.org/10.1021/acs.jproteome.6b00354
Bian X, Wasserfall C, Wallstrom G, Wang J, Wang H, Barker K et al. Tracking the Antibody Immunome in Type 1 Diabetes Using Protein Arrays. Journal of Proteome Research. 2017 Jan 6;16(1):195-203. https://doi.org/10.1021/acs.jproteome.6b00354
Bian, Xiaofang ; Wasserfall, Clive ; Wallstrom, Garrick ; Wang, Jie ; Wang, Haoyu ; Barker, Kristi ; Schatz, Desmond ; Atkinson, Mark ; Qiu, Ji ; LaBaer, Joshua. / Tracking the Antibody Immunome in Type 1 Diabetes Using Protein Arrays. In: Journal of Proteome Research. 2017 ; Vol. 16, No. 1. pp. 195-203.
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