Toxic role of K + channel oxidation in mammalian brain

Diego Cotella, Berenice Hernandez-Enriquez, Xilong Wu, Ruiqiong Li, Zui Pan, Joseph Leveille, Christopher D. Link, Salvatore Oddo, Federico Sesti

Research output: Contribution to journalArticle

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Abstract

Potassium (K +) channels are essential to neuronal signaling and survival. Here we show that these proteins are targets of reactive oxygen species in mammalian brain and that their oxidation contributes to neuropathy. Thus, the KCNB1 (Kv2.1) channel, which is abundantly expressed in cortex and hippocampus, formed oligomers upon exposure to oxidizing agents. These oligomers were ~ 10-fold more abundant in the brain of old than young mice. Oxidant-induced oligomerization of wild-type KCNB1 enhanced apoptosis in neuronal cells subject to oxidative insults. Consequently, a KCNB1 variant resistant to oxidation, obtained by mutating a conserved cysteine to alanine, (C73A), was neuroprotective. The fact that oxidation of KCNB1 is toxic, argues that this mechanism may contribute to neuropathy in conditions characterized by high levels of oxidative stress, such as Alzheimer's disease (AD). Accordingly, oxidation of KCNB1 channels was exacerbated in the brain of a triple transgenic mouse model of AD (3xTg-AD). The C73A variant protected neuronal cells from apoptosis induced by incubation with /β-amyloid peptide (Aβ 1-42). In an invertebrate model (Caenorhabditis elegans) that mimics aspects of AD, a C73A-KCNB1 homolog (C113S-KVS-1) protected specific neurons from apoptotic death induced by ectopic expression of human Aβ 1-42. Together, these data underscore a novel mechanism of toxicity in neurodegenerative disease.

Original languageEnglish (US)
Pages (from-to)4133-4144
Number of pages12
JournalJournal of Neuroscience
Volume32
Issue number12
DOIs
StatePublished - Mar 21 2012
Externally publishedYes

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Poisons
Alzheimer Disease
Brain
Oxidants
Apoptosis
Potassium Channels
Caenorhabditis elegans
Invertebrates
Amyloid
Neurodegenerative Diseases
Alanine
Transgenic Mice
Cysteine
Reactive Oxygen Species
Hippocampus
Oxidative Stress
Neurons
Survival
Proteins

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Cotella, D., Hernandez-Enriquez, B., Wu, X., Li, R., Pan, Z., Leveille, J., ... Sesti, F. (2012). Toxic role of K + channel oxidation in mammalian brain. Journal of Neuroscience, 32(12), 4133-4144. https://doi.org/10.1523/JNEUROSCI.6153-11.2012

Toxic role of K + channel oxidation in mammalian brain. / Cotella, Diego; Hernandez-Enriquez, Berenice; Wu, Xilong; Li, Ruiqiong; Pan, Zui; Leveille, Joseph; Link, Christopher D.; Oddo, Salvatore; Sesti, Federico.

In: Journal of Neuroscience, Vol. 32, No. 12, 21.03.2012, p. 4133-4144.

Research output: Contribution to journalArticle

Cotella, D, Hernandez-Enriquez, B, Wu, X, Li, R, Pan, Z, Leveille, J, Link, CD, Oddo, S & Sesti, F 2012, 'Toxic role of K + channel oxidation in mammalian brain', Journal of Neuroscience, vol. 32, no. 12, pp. 4133-4144. https://doi.org/10.1523/JNEUROSCI.6153-11.2012
Cotella D, Hernandez-Enriquez B, Wu X, Li R, Pan Z, Leveille J et al. Toxic role of K + channel oxidation in mammalian brain. Journal of Neuroscience. 2012 Mar 21;32(12):4133-4144. https://doi.org/10.1523/JNEUROSCI.6153-11.2012
Cotella, Diego ; Hernandez-Enriquez, Berenice ; Wu, Xilong ; Li, Ruiqiong ; Pan, Zui ; Leveille, Joseph ; Link, Christopher D. ; Oddo, Salvatore ; Sesti, Federico. / Toxic role of K + channel oxidation in mammalian brain. In: Journal of Neuroscience. 2012 ; Vol. 32, No. 12. pp. 4133-4144.
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