TNA synthesis by DNA polymerases

John C. Chaput, Jack W. Szostak

Research output: Contribution to journalArticle

103 Citations (Scopus)

Abstract

Threose nucleic acid (TNA), which has a repeat unit one atom shorter than that of DNA, is capable of Watson-Crick base pairing with DNA, RNA, and TNA. Because of its chemical simplicity, TNA is considered to be a possible progenitor of RNA. As an initial step toward developing the molecular tools necessary to investigate the functional capabilities of TNA by in vitro selection, we have screened a variety of DNA polymerases for TNA synthesis activity on a DNA template. We wish to report that several polymerases show surprisingly good ability to synthesize TNA using α-l-threofuranosyl thymidine-3′-triphosphate as a substrate.

Original languageEnglish (US)
Pages (from-to)9274-9275
Number of pages2
JournalJournal of the American Chemical Society
Volume125
Issue number31
DOIs
StatePublished - Aug 6 2003
Externally publishedYes

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Nucleic acids
DNA-Directed DNA Polymerase
Nucleic Acids
DNA
RNA
Base Pairing
Thymidine
erythrose
Atoms
Substrates

ASJC Scopus subject areas

  • Chemistry(all)

Cite this

TNA synthesis by DNA polymerases. / Chaput, John C.; Szostak, Jack W.

In: Journal of the American Chemical Society, Vol. 125, No. 31, 06.08.2003, p. 9274-9275.

Research output: Contribution to journalArticle

Chaput, JC & Szostak, JW 2003, 'TNA synthesis by DNA polymerases', Journal of the American Chemical Society, vol. 125, no. 31, pp. 9274-9275. https://doi.org/10.1021/ja035917n
Chaput, John C. ; Szostak, Jack W. / TNA synthesis by DNA polymerases. In: Journal of the American Chemical Society. 2003 ; Vol. 125, No. 31. pp. 9274-9275.
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