Thin-layer chromatography of bryostatins

Ayano Yamashita, Yoshiaki Kamano, Toshihiko Nogawa, Hatsue Hiraide, Taeko Kazama, Machiko Tozawa, Cherry L. Herald, George Pettit

Research output: Contribution to journalArticle

Abstract

The separation of eleven pure bryostatins by thin-layer chromatography has been accomplished on silica gel plates with n-hexane-acetone, 7 + 3, and n-hexane - ethyl acetate, 1 + 1, for normal phase chromatography and methanol-water, 3 + 1, for reversed phase chromatography. The technique was suitable for analysis of bryostatins in bioactive fractions from extracts of the marine bryozoan Bugula neritina. The method was also used in support of structure elucidation, the acetylation of bryostatins 1 and 2, and the hydrolysis of acetates; the hydrolysis of bryostatins 4-11 was also studied by TLC.

Original languageEnglish (US)
Pages (from-to)336-339
Number of pages4
JournalJournal of Planar Chromatography - Modern TLC
Volume12
Issue number5
StatePublished - Sep 1999

Fingerprint

Bryostatins
Thin layer chromatography
Thin Layer Chromatography
Chromatography
Hydrolysis
Acetylation
Silica Gel
Reverse-Phase Chromatography
Acetone
Methanol
Acetates
Water
n-hexane

Keywords

  • Bryostatins
  • Bugula neritina
  • Multiple one-dimensional separation
  • Multiple two-dimensional separation
  • Thin-layer chromatography

ASJC Scopus subject areas

  • Analytical Chemistry

Cite this

Yamashita, A., Kamano, Y., Nogawa, T., Hiraide, H., Kazama, T., Tozawa, M., ... Pettit, G. (1999). Thin-layer chromatography of bryostatins. Journal of Planar Chromatography - Modern TLC, 12(5), 336-339.

Thin-layer chromatography of bryostatins. / Yamashita, Ayano; Kamano, Yoshiaki; Nogawa, Toshihiko; Hiraide, Hatsue; Kazama, Taeko; Tozawa, Machiko; Herald, Cherry L.; Pettit, George.

In: Journal of Planar Chromatography - Modern TLC, Vol. 12, No. 5, 09.1999, p. 336-339.

Research output: Contribution to journalArticle

Yamashita, A, Kamano, Y, Nogawa, T, Hiraide, H, Kazama, T, Tozawa, M, Herald, CL & Pettit, G 1999, 'Thin-layer chromatography of bryostatins', Journal of Planar Chromatography - Modern TLC, vol. 12, no. 5, pp. 336-339.
Yamashita A, Kamano Y, Nogawa T, Hiraide H, Kazama T, Tozawa M et al. Thin-layer chromatography of bryostatins. Journal of Planar Chromatography - Modern TLC. 1999 Sep;12(5):336-339.
Yamashita, Ayano ; Kamano, Yoshiaki ; Nogawa, Toshihiko ; Hiraide, Hatsue ; Kazama, Taeko ; Tozawa, Machiko ; Herald, Cherry L. ; Pettit, George. / Thin-layer chromatography of bryostatins. In: Journal of Planar Chromatography - Modern TLC. 1999 ; Vol. 12, No. 5. pp. 336-339.
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