The telomere binding protein trf2 induces chromatin compaction

Asmaa M. Baker, Qiang Fu, William Hayward, Samuel Victoria, Ilene M. Pedroso, Stuart Lindsay, Terace M. Fletcher

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Mammalian telomeres are specialized chromatin structures that require the telomere binding protein, TRF2, for maintaining chromosome stability. In addition to its ability to modulate DNA repair activities, TRF2 also has direct effects on DNA structure and topology. Given that mammalian telomeric chromatin includes nucleosomes, we investigated the effect of this protein on chromatin structure. TRF2 bound to reconstituted telomeric nucleosomal fibers through both its basic N-terminus and its C-terminal DNA binding domain. Analytical agarose gel electrophoresis (AAGE) studies showed that TRF2 promoted the folding of nucleosomal arrays into more compact structures by neutralizing negative surface charge. A construct containing the N-terminal and TRFH domains together altered the charge and radius of nucleosomal arrays similarly to full-length TRF2 suggesting that TRF2-driven changes in global chromatin structure were largely due to these regions. However, the most compact chromatin structures were induced by the isolated basic N-terminal region, as judged by both AAGE and atomic force microscopy. Although the N-terminal region condensed nucleosomal array fibers, the TRFH domain, known to alter DNA topology, was required for stimulation of a strand invasion-like reaction with nucleosomal arrays. Optimal strand invasion also required the C-terminal DNA binding domain. Furthermore, the reaction was not stimulated on linear histone-free DNA. Our data suggest that nucleosomal chromatin has the ability to facilitate this activity of TRF2 which is thought to be involved in stabilizing looped telomere structures.

Original languageEnglish (US)
Article numbere19124
JournalPLoS One
Volume6
Issue number4
DOIs
StatePublished - 2011

Fingerprint

Telomere-Binding Proteins
telomeres
Chromatin
chromatin
binding proteins
Compaction
DNA
DNA-binding domains
Agar Gel Electrophoresis
Telomere
Electrophoresis
Sepharose
topology
agarose
gel electrophoresis
Gels
Topology
Chromosomal Instability
nucleosomes
atomic force microscopy

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Baker, A. M., Fu, Q., Hayward, W., Victoria, S., Pedroso, I. M., Lindsay, S., & Fletcher, T. M. (2011). The telomere binding protein trf2 induces chromatin compaction. PLoS One, 6(4), [e19124]. https://doi.org/10.1371/journal.pone.0019124

The telomere binding protein trf2 induces chromatin compaction. / Baker, Asmaa M.; Fu, Qiang; Hayward, William; Victoria, Samuel; Pedroso, Ilene M.; Lindsay, Stuart; Fletcher, Terace M.

In: PLoS One, Vol. 6, No. 4, e19124, 2011.

Research output: Contribution to journalArticle

Baker, AM, Fu, Q, Hayward, W, Victoria, S, Pedroso, IM, Lindsay, S & Fletcher, TM 2011, 'The telomere binding protein trf2 induces chromatin compaction', PLoS One, vol. 6, no. 4, e19124. https://doi.org/10.1371/journal.pone.0019124
Baker, Asmaa M. ; Fu, Qiang ; Hayward, William ; Victoria, Samuel ; Pedroso, Ilene M. ; Lindsay, Stuart ; Fletcher, Terace M. / The telomere binding protein trf2 induces chromatin compaction. In: PLoS One. 2011 ; Vol. 6, No. 4.
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