The structure of ferritin cores determined by electron nanodiffraction

J. M. Cowley, Dawn E. Janney, Richard Gerkin, P R Buseck

Research output: Contribution to journalArticle

100 Citations (Scopus)

Abstract

Electron nanodiffraction, with a 100-keV electron beam less than 1 nm in diameter, has been used to obtain single-crystal diffraction patterns from individual iron-containing cores of ferritin molecules. We show that, while a majority of the cores have a hexagonal structure somewhat similar to the major phase in the mineral ferrihydrite, as previously assumed, several minor phases are present including some that are similar in structure to the iron oxides magnetite and hematite and also some composed of highly disordered material. In general, each core consists of one single crystal of one phase. (C) 2000 Academic Press.

Original languageEnglish (US)
Pages (from-to)210-216
Number of pages7
JournalJournal of Structural Biology
Volume131
Issue number3
DOIs
StatePublished - 2000

Fingerprint

Ferritins
Electrons
Ferrosoferric Oxide
Minerals
Iron
ferric oxide
ferrihydrite

Keywords

  • Electron nanodiffraction
  • Ferrihydrite
  • Ferritin cores
  • Iron oxides
  • Scanning transmission electron microscopy
  • Structural disorder

ASJC Scopus subject areas

  • Structural Biology

Cite this

The structure of ferritin cores determined by electron nanodiffraction. / Cowley, J. M.; Janney, Dawn E.; Gerkin, Richard; Buseck, P R.

In: Journal of Structural Biology, Vol. 131, No. 3, 2000, p. 210-216.

Research output: Contribution to journalArticle

@article{3d9badfc45164292a94772c415032888,
title = "The structure of ferritin cores determined by electron nanodiffraction",
abstract = "Electron nanodiffraction, with a 100-keV electron beam less than 1 nm in diameter, has been used to obtain single-crystal diffraction patterns from individual iron-containing cores of ferritin molecules. We show that, while a majority of the cores have a hexagonal structure somewhat similar to the major phase in the mineral ferrihydrite, as previously assumed, several minor phases are present including some that are similar in structure to the iron oxides magnetite and hematite and also some composed of highly disordered material. In general, each core consists of one single crystal of one phase. (C) 2000 Academic Press.",
keywords = "Electron nanodiffraction, Ferrihydrite, Ferritin cores, Iron oxides, Scanning transmission electron microscopy, Structural disorder",
author = "Cowley, {J. M.} and Janney, {Dawn E.} and Richard Gerkin and Buseck, {P R}",
year = "2000",
doi = "10.1006/jsbi.2000.4292",
language = "English (US)",
volume = "131",
pages = "210--216",
journal = "Journal of Structural Biology",
issn = "1047-8477",
publisher = "Academic Press Inc.",
number = "3",

}

TY - JOUR

T1 - The structure of ferritin cores determined by electron nanodiffraction

AU - Cowley, J. M.

AU - Janney, Dawn E.

AU - Gerkin, Richard

AU - Buseck, P R

PY - 2000

Y1 - 2000

N2 - Electron nanodiffraction, with a 100-keV electron beam less than 1 nm in diameter, has been used to obtain single-crystal diffraction patterns from individual iron-containing cores of ferritin molecules. We show that, while a majority of the cores have a hexagonal structure somewhat similar to the major phase in the mineral ferrihydrite, as previously assumed, several minor phases are present including some that are similar in structure to the iron oxides magnetite and hematite and also some composed of highly disordered material. In general, each core consists of one single crystal of one phase. (C) 2000 Academic Press.

AB - Electron nanodiffraction, with a 100-keV electron beam less than 1 nm in diameter, has been used to obtain single-crystal diffraction patterns from individual iron-containing cores of ferritin molecules. We show that, while a majority of the cores have a hexagonal structure somewhat similar to the major phase in the mineral ferrihydrite, as previously assumed, several minor phases are present including some that are similar in structure to the iron oxides magnetite and hematite and also some composed of highly disordered material. In general, each core consists of one single crystal of one phase. (C) 2000 Academic Press.

KW - Electron nanodiffraction

KW - Ferrihydrite

KW - Ferritin cores

KW - Iron oxides

KW - Scanning transmission electron microscopy

KW - Structural disorder

UR - http://www.scopus.com/inward/record.url?scp=0033767569&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033767569&partnerID=8YFLogxK

U2 - 10.1006/jsbi.2000.4292

DO - 10.1006/jsbi.2000.4292

M3 - Article

VL - 131

SP - 210

EP - 216

JO - Journal of Structural Biology

JF - Journal of Structural Biology

SN - 1047-8477

IS - 3

ER -