The same normal cell protein is phosphorylated after transformation by avian sarcoma viruses with unrelated transforming genes

E. Erikson, Robert Cook-Deegan, G. J. Miller, R. L. Erikson

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

The phosphorylation of a normal cellular protein of molecular weight 34,000 (34K) is enhanced in Rous sarcoma virus-transformed chicken embryo fibroblasts apparently as a direct consequence of the phosphotransferase activity of the Rous sarcoma virus-transforming protein pp60(src). We have prepared anti-34K serum by using 34K purified from normal fibroblasts to confirm that the transformation-specific phosphorylation described previously occurs on a normal cellular protein and to further characterize the nature of the protein. In this communication, we also show that the phosphorylation of 34K is also increased in cells transformed by either Fujinami or PRCII sarcoma virus, two recently characterized avian sarcoma viruses whose transforming proteins, although distinct from pp60(src), are also associated with phosphotransferase activity. Moreover, comparative fingerprinting of tryptic phosphopeptides shows that the major site of phosphorylation of 34K is the same in all three cases.

Original languageEnglish (US)
Pages (from-to)43-50
Number of pages8
JournalMolecular and Cellular Biology
Volume1
Issue number1
StatePublished - 1981
Externally publishedYes

Fingerprint

Avian Sarcoma Viruses
Oncogenes
Phosphorylation
Rous sarcoma virus
Proteins
Oncogene Protein pp60(v-src)
Phosphotransferases
Fibroblasts
Phosphopeptides
Sarcoma
Chickens
Embryonic Structures
Molecular Weight
Viruses
Serum

ASJC Scopus subject areas

  • Cell Biology
  • Genetics
  • Molecular Biology

Cite this

The same normal cell protein is phosphorylated after transformation by avian sarcoma viruses with unrelated transforming genes. / Erikson, E.; Cook-Deegan, Robert; Miller, G. J.; Erikson, R. L.

In: Molecular and Cellular Biology, Vol. 1, No. 1, 1981, p. 43-50.

Research output: Contribution to journalArticle

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