Abstract
Phytochromes are a family of photoreceptors that control light responses of plants, fungi and bacteria. A sequence of structural changes, which is not yet fully understood, leads to activation of an output domain. Time-resolved serial femtosecond crystallography (SFX) can potentially shine light on these conformational changes. Here we report the room temperature crystal structure of the chromophore-binding domains of the Deinococcus radiodurans phytochrome at 2.1 Å resolution. The structure was obtained by serial femtosecond X-ray crystallography from microcrystals at an X-ray free electron laser. We find overall good agreement compared to a crystal structure at 1.35 Å resolution derived from conventional crystallography at cryogenic temperatures, which we also report here. The thioether linkage between chromophore and protein is subject to positional ambiguity at the synchrotron, but is fully resolved with SFX. The study paves the way for time-resolved structural investigations of the phytochrome photocycle with time-resolved SFX.
Original language | English (US) |
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Article number | 35279 |
Journal | Scientific reports |
Volume | 6 |
DOIs | |
State | Published - Oct 19 2016 |
ASJC Scopus subject areas
- General
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Wild-type PAS-GAF fragment from Deinococcus radiodurans BphP
Edlund, P. (Contributor), Takala, H. (Contributor), Claesson, E. (Contributor), Henry, L. (Contributor), Dods, R. (Contributor), Lehtivuori, H. (Contributor), Panman, M. (Contributor), Pande, K. (Contributor), White, T. (Contributor), Nakane, T. (Contributor), Berntsson, O. (Contributor), Gustavsson, E. (Contributor), Båth, P. (Contributor), Modi, V. (Contributor), Roy-Chowdhury, S. (Contributor), Zook, J. (Contributor), Berntsen, P. (Contributor), Pandey, S. (Contributor), Poudyal, I. (Contributor), Tenboer, J. (Contributor), Kupitz, C. (Contributor), Barty, A. (Contributor), Fromme, P. (Contributor), Koralek, J. D. (Contributor), Tanaka, T. (Contributor), Spence, J. (Contributor), Liang, M. (Contributor), Hunter, M. S. (Contributor), Boutet, S. (Contributor), Nango, E. (Contributor), Moffat, K. (Contributor), Groenhof, G. (Contributor), Ihalainen, J. (Contributor), Stojkovi, E. A. (Contributor), Schmidt, M. (Contributor) & Westenhoff, S. (Contributor), Protein Data Bank (PDB), Oct 26 2016
DOI: 10.2210/pdb5K5B, https://www.wwpdb.org/pdb?id=pdb_00005k5b
Dataset
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Wild-type PAS-GAF fragment from Deinococcus radiodurans Bphp collected at SACLA
Edlund, P. (Contributor), Takala, H. (Contributor), Claesson, E. (Contributor), Henry, L. (Contributor), Dods, R. (Contributor), Lehtivuori, H. (Contributor), Panman, M. (Contributor), Pande, K. (Contributor), White, T. (Contributor), Nakane, T. (Contributor), Berntsson, O. (Contributor), Gustavsson, E. (Contributor), Båth, P. (Contributor), Modi, V. (Contributor), Roy-Chowdhury, S. (Contributor), Zook, J. (Contributor), Berntsen, P. (Contributor), Pandey, S. (Contributor), Poudyal, I. (Contributor), Tenboer, J. (Contributor), Kupitz, C. (Contributor), Barty, A. (Contributor), Fromme, P. (Contributor), Koralek, J. D. (Contributor), Tanaka, T. (Contributor), Spence, J. (Contributor), Liang, M. (Contributor), Hunter, M. S. (Contributor), Boutet, S. (Contributor), Nango, E. (Contributor), Moffat, K. (Contributor), Groenhof, G. (Contributor), Ihalainen, J. (Contributor), Stojkovi, E. A. (Contributor), Schmidt, M. (Contributor) & Westenhoff, S. (Contributor), Protein Data Bank (PDB), Oct 26 2016
DOI: 10.2210/pdb5LBR, https://www.wwpdb.org/pdb?id=pdb_00005lbr
Dataset
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Wild-type PAS-GAF fragment from Deinococcus radiodurans Bphp collected at LCLS
Edlund, P. (Contributor), Takala, H. (Contributor), Claesson, E. (Contributor), Henry, L. (Contributor), Dods, R. (Contributor), Lehtivuori, H. (Contributor), Panman, M. (Contributor), Pande, K. (Contributor), White, T. (Contributor), Nakane, T. (Contributor), Berntsson, O. (Contributor), Gustavsson, E. (Contributor), Båth, P. (Contributor), Modi, V. (Contributor), Roy-Chowdhury, S. (Contributor), Zook, J. (Contributor), Berntsen, P. (Contributor), Pandey, S. (Contributor), Poudyal, I. (Contributor), Tenboer, J. (Contributor), Kupitz, C. (Contributor), Barty, A. (Contributor), Fromme, P. (Contributor), Koralek, J. D. (Contributor), Tanaka, T. (Contributor), Spence, J. (Contributor), Liang, M. (Contributor), Hunter, M. S. (Contributor), Boutet, S. (Contributor), Nango, E. (Contributor), Moffat, K. (Contributor), Groenhof, G. (Contributor), Ihalainen, J. (Contributor), Stojkovi, E. A. (Contributor), Schmidt, M. (Contributor) & Westenhoff, S. (Contributor), Protein Data Bank (PDB), Oct 26 2016
DOI: 10.2210/pdb5L8M, https://www.wwpdb.org/pdb?id=pdb_00005l8m
Dataset