The myxoma virus M-T5 ankyrin repeat host range protein is a novel adaptor that coordinately links the cellular signaling pathways mediated by Akt and Skp1 in virus-infected cells

Steven J. Werden, Jerry Lanchbury, Donna Shattuck, Chris Neff, Max Dufford, Douglas McFadden

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Most poxviruses express multiple proteins containing ankyrin (ANK) repeats accounting for a large superfamily of related but unique determinants of poxviral tropism. Recently, select members of this novel family of poxvirus proteins have drawn considerable attention for their potential roles in modulating intracellular signaling networks during viral infection. The rabbit-specific poxvirus, myxoma virus (MYXV), encodes four unique ANK repeat proteins, termed M-T5, M148, M149, and M150, all of which include a carboxy-terminal PRANC domain which closely resembles a cellular protein motif called the F-box domain. Here, we show that each MYXV-encoded ANK repeat protein, including M-T5, interacts directly with the Skp1 component of the host SCF ubiquitin ligase complex, and that the binding of M-T5 to cullin 1 is indirect via binding to Skp1 in the host SCF complex. To understand the significance of these virus-host protein interactions, the various binding domains of M-T5 were mapped. The N-terminal ANK repeats I and II were identified as being important for interaction with Akt, whereas the C-terminal PRANC/F-box-like domain was essential for binding to Skp1. We also report that M-T5 can bind Akt and the host SCF complex (via Skp1) simultaneously in MYXV-infected cells. Finally, we report that M-T5 specifically mediates the relocalization of Akt from the nucleus to the cytoplasm during infection with the wild-type MYXV, but not the M-T5 knockout version of the virus. These results indicate that ANK/PRANC proteins play a critical role in reprogramming disparate cellular signaling cascades to establish a new cellular environment more favorable for virus replication.

Original languageEnglish (US)
Pages (from-to)12068-12083
Number of pages16
JournalJournal of Virology
Volume83
Issue number23
DOIs
StatePublished - Dec 1 2009
Externally publishedYes

Fingerprint

Myxoma virus
Ankyrin Repeat
ankyrins
cell communication
Host Specificity
host range
Viruses
Poxviridae
viruses
Proteins
proteins
cells
SKP Cullin F-Box Protein Ligases
Ankyrins
Amino Acid Motifs
Tropism
Terminal Repeat Sequences
Virus Diseases
tropisms
Virus Replication

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

Cite this

The myxoma virus M-T5 ankyrin repeat host range protein is a novel adaptor that coordinately links the cellular signaling pathways mediated by Akt and Skp1 in virus-infected cells. / Werden, Steven J.; Lanchbury, Jerry; Shattuck, Donna; Neff, Chris; Dufford, Max; McFadden, Douglas.

In: Journal of Virology, Vol. 83, No. 23, 01.12.2009, p. 12068-12083.

Research output: Contribution to journalArticle

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