The highly specific and sensitive analysis of repertoire of serum antibodies using peptide microchips in patients with diagnosis of breast cancer

S. V. Podlesnykh, E. A. Kolosova, D. S. Anisimov, D. N. Scherbakov, M. A. Ryazanov, V. D. Petrova, Ya N. Shoikhet, Stephen Johnston, A. F. Lazarev, Andrei Chapoval

Research output: Contribution to journalArticle

Abstract

Nowadays, the search for new biomarkers of cancer is actual for early diagnostic. The antibodies against antigens associated with tumors arouse an interest as an alternative to the present biomarkers. The alteration of quantity and specificity of antibodies in blood serum indicates processes occurring in the organism, including development of oncologic diseases. The article presents the results of analysis of repertoire of antibodies (immunosignatures) using peptide micro-chips inpatients with diagnosis of breast cancer. The study established 119 informative peptides out of 330 034 peptides, presented on the microchip. The binding of antibodies with them demonstrates statistically significant intergroup values (p<0.001). This panel of peptides is effective in discerning sera of patients and healthy donors with high sensitivity and specificity. The evaluation of repertoire of antibodies interacting with panel of synthetic peptides can be used in development of diagnostic tests in case of detection of breast cancer at early stages.

Original languageEnglish (US)
Pages (from-to)557-563
Number of pages7
JournalKlinichescheskaya Laboratornaya Diagnostika
Volume62
Issue number9
DOIs
StatePublished - 2017

Keywords

  • Biomarkers
  • Breast cancer
  • Immunosignature/0869-2084-2017-62-10-
  • Peptide microchips
  • Repertoire of antibodies
  • Tumor-associated antibodies

ASJC Scopus subject areas

  • Medical Laboratory Technology
  • Biochemistry, medical

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