The gene structure of the Drosophila melanogaster proto-oncogene, kayak, and its nested gene, fos-intronic gene

Stephanie Gidget Hudson, Elliott S. Goldstein

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

We present herein a new model for the structure of the Drosophila kayak gene as well as preliminary data on the functional differences of its various isoforms. kayak is a homolog of the human proto-oncogene, c-fos. kayak has three different starts of transcription, and therefore promoters (P)kay-α, (P)kay-β and (P)kay-γ. These three promoters lead to four different transcripts: kay-α, kaysro, kay-β and kay-γ. (P)kay-α produces two different transcripts: kay-α and kaysro where the other two promoters, (P)kay-β and (P)kay-γ, produce a single transcript each. The transcripts kay-α, β and γ all splice into the mainbody of the kay gene, which codes for the DNA binding domain and leucine zipper; kaysro is not spliced. Also, within this region is a nested gene, fos-intronic gene (fig) which is transcribed in the opposite direction. fig codes for a predicted PP2C phosphatase. fig has two different promoters which produce two different transcripts, both in the same reading frame, fig-α and β. This is an unusual gene structure for Drosophila. Only 13% of Drosophila genes have multiple promoters and only 7% have a nested gene. RT-PCR was performed on each transcript to determine the relative amounts of each RNA produced. All spliced kay transcripts appear to have equal abundance. The unspliced kaysro transcript has a lower abundance than kay-α. Both fig transcripts are also detected in all stages tested. Lethal phase analysis and complementation testing suggest that the three isoforms of kayak may have different functions.

Original languageEnglish (US)
Pages (from-to)76-81
Number of pages6
JournalGene
Volume420
Issue number1
DOIs
StatePublished - Aug 15 2008

Keywords

  • AP-1
  • Fos
  • Transcription factor

ASJC Scopus subject areas

  • Genetics

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