The gene csd is the primary signal for sexual development in the honeybee and encodes an SR-type protein

Martin Beye; Martin Hasselmann; M. Kim Fondrk; Robert E. Page; Stig W. Omholt

doi:10.1016/S0092-8674(03)00606-8

The gene csd is the primary signal for sexual development in the honeybee and encodes an SR-type protein

Martin Beye, Martin Hasselmann, M. Kim Fondrk, Robert E. Page, Stig W. Omholt

Research output: Contribution to journal › Article › peer-review

403 Scopus citations

Abstract

Haplodiploid organisms comprise about 20% of animals. Males develop from unfertilized eggs while females are derived from fertilized eggs. The underlying mechanisms of sex determination, however, appear to be diverse and are poorly understood. We have dissected the complementary sex determiner (csd) locus in the honeybee to understand its molecular basis. In this species, csd acts as the primary sex-determining signal with several alleles segregating in populations. Males are hemizygous and females are heterozygous at this locus; nonreproducing diploid males occur when the locus is homozygous. We have characterized csd by positional cloning and repression analysis. csd alleles are highly variable and no transcription differences were found between sexes. These results establish csd as a primary signal that governs sexual development by its allelic composition. Structural similarity of csd with tra genes of Dipteran insects suggests some functional relation of what would otherwise appear to be unrelated sex-determination mechanisms.

Original language	English (US)
Pages (from-to)	419-429
Number of pages	11
Journal	Cell
Volume	114
Issue number	4
DOIs	https://doi.org/10.1016/S0092-8674(03)00606-8
State	Published - Aug 22 2003
Externally published	Yes

ASJC Scopus subject areas

General Biochemistry, Genetics and Molecular Biology

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10.1016/S0092-8674(03)00606-8

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title = "The gene csd is the primary signal for sexual development in the honeybee and encodes an SR-type protein",

abstract = "Haplodiploid organisms comprise about 20% of animals. Males develop from unfertilized eggs while females are derived from fertilized eggs. The underlying mechanisms of sex determination, however, appear to be diverse and are poorly understood. We have dissected the complementary sex determiner (csd) locus in the honeybee to understand its molecular basis. In this species, csd acts as the primary sex-determining signal with several alleles segregating in populations. Males are hemizygous and females are heterozygous at this locus; nonreproducing diploid males occur when the locus is homozygous. We have characterized csd by positional cloning and repression analysis. csd alleles are highly variable and no transcription differences were found between sexes. These results establish csd as a primary signal that governs sexual development by its allelic composition. Structural similarity of csd with tra genes of Dipteran insects suggests some functional relation of what would otherwise appear to be unrelated sex-determination mechanisms.",

author = "Martin Beye and Martin Hasselmann and Fondrk, {M. Kim} and Page, {Robert E.} and Omholt, {Stig W.}",

note = "Funding Information: We thank R. N{\"o}thiger, D. Bopp, A. Wilkins, and three anonymous reviewers for critical review of the manuscript. The authors are very grateful to G. Hunt and M. Solignac for access to BAC libraries; B. Polaczek and B. Schricker for providing eggs; A. Peterson for sequencing; T. Gempe for help with cDNA synthesis; and G. Amdam and R. Becher for assisting with bee management. This research was funded by grants from the Deutsche Forschungsgemeinschaft (2194/3) and the Kultusminister of Sachsen-Anhalt (3266A/0020L) to M.B. and grants from the U.S. National Science Foundation to REP (IBN 0090482 and IBN 0076811). The collaboration with R.E.P. was made possible by an Alexander von Humboldt Foundation Feodor-Lynen-Fellowship to M.B. ",

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AU - Beye, Martin

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AU - Fondrk, M. Kim

AU - Page, Robert E.

AU - Omholt, Stig W.

N1 - Funding Information: We thank R. Nöthiger, D. Bopp, A. Wilkins, and three anonymous reviewers for critical review of the manuscript. The authors are very grateful to G. Hunt and M. Solignac for access to BAC libraries; B. Polaczek and B. Schricker for providing eggs; A. Peterson for sequencing; T. Gempe for help with cDNA synthesis; and G. Amdam and R. Becher for assisting with bee management. This research was funded by grants from the Deutsche Forschungsgemeinschaft (2194/3) and the Kultusminister of Sachsen-Anhalt (3266A/0020L) to M.B. and grants from the U.S. National Science Foundation to REP (IBN 0090482 and IBN 0076811). The collaboration with R.E.P. was made possible by an Alexander von Humboldt Foundation Feodor-Lynen-Fellowship to M.B.

PY - 2003/8/22

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N2 - Haplodiploid organisms comprise about 20% of animals. Males develop from unfertilized eggs while females are derived from fertilized eggs. The underlying mechanisms of sex determination, however, appear to be diverse and are poorly understood. We have dissected the complementary sex determiner (csd) locus in the honeybee to understand its molecular basis. In this species, csd acts as the primary sex-determining signal with several alleles segregating in populations. Males are hemizygous and females are heterozygous at this locus; nonreproducing diploid males occur when the locus is homozygous. We have characterized csd by positional cloning and repression analysis. csd alleles are highly variable and no transcription differences were found between sexes. These results establish csd as a primary signal that governs sexual development by its allelic composition. Structural similarity of csd with tra genes of Dipteran insects suggests some functional relation of what would otherwise appear to be unrelated sex-determination mechanisms.

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The gene csd is the primary signal for sexual development in the honeybee and encodes an SR-type protein

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