The fate of cytochrome b559 during anaerobic photoinhibition and its recovery processes

Tsafrir S. Mor, Torill Hundal, Itzhak Ohad, Bertil Andersson

Research output: Contribution to journalArticle

9 Scopus citations

Abstract

The function of the cytochrome b559, a Photosystem II (PS II) reaction center ubiquitous component is not yet known. Cytochrome b559 appears in a high (HP) or low (LP) potential form. The HP form is converted into the LP form during aerobic photoinhibition. It has been proposed before that this conversion, assumed to be reversible, ascribes protection against light stress of PS II by redirecting electron flow within PS II thus avoiding charge recombination of the primary radical pair and related oxidative damage. Here, we have used an experimental system allowing to assay the relation between the cytochrome b559 redox potential shift, its reversibility and protection against light induced PS II inactivation. Under anaerobic conditions fast reversible photoinactivation of PS II in isolated spinach thylakoids is observed accompanied by monomerisation of PS II. Monomers did not dissociate further into PS II sub-particles and did not migrate out of the grana partitions as observed in aerobic photoinactivation. The anaerobic photoinactivation is accompanied by an increase in the cytochrome b559 Lp/HP ratio. However, despite recovery of PS II activity and partially of its dimeric form in darkness under aerobic conditions, no reversal of the cytochrome b559 redox potential shift accompanied these processes. Re-exposure of reactivated thylakoids having an increased PS II population in the LP form of the cytochrome b559 to strong illumination under aerobic conditions, did not result in a measurable protection of PS II as compared to control thylakoids. While it is possible that cytochrome b559 may play a protective role against light stress in PS II, the results presented here do not indicate that the increase in the ratio LP/HP form is involved in this process.

Original languageEnglish (US)
Pages (from-to)205-213
Number of pages9
JournalPhotosynthesis research
Volume53
Issue number2-3
StatePublished - Jan 1 1997
Externally publishedYes

Keywords

  • Photosystem II monomers/dimers
  • Protective mechanisms

ASJC Scopus subject areas

  • Biochemistry
  • Plant Science
  • Cell Biology

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