The effect of carotenoid supplementation on immune system development in juvenile male veiled chameleons (Chamaeleo calyptratus)

Kristen L. McCartney, Russell A. Ligon, Michael W. Butler, Dale Denardo, Kevin McGraw

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Introduction: Nutrient availability, assimilation, and allocation can have important and lasting effects on the immune system development of growing animals. Though carotenoid pigments have immunostimulatory properties in many animals, relatively little is known regarding how they influence the immune system during development. Moreover, studies linking carotenoids to health at any life stage have largely been restricted to birds and mammals. We investigated the effects of carotenoid supplementation on multiple aspects of immunity in juvenile veiled chameleons (Chamaeleo calyptratus). We supplemented half of the chameleons with lutein (a xanthophyll carotenoid) for 14 weeks during development and serially measured multiple aspects of immune function, including: agglutination and lysis performance of plasma, wound healing, and plasma nitric oxide concentrations before and after wounding.Results: Though lutein supplementation effectively elevated circulating carotenoid concentrations throughout the developmental period, we found no evidence that carotenoid repletion enhanced immune function at any point. However, agglutination and lysis scores increased, while baseline nitric oxide levels decreased, as chameleons aged.Conclusions: Taken together, our results indicate that body mass and age, but not carotenoid access, may play an important role in immune performance of growing chameleons. Hence, studying well-understood physiological processes in novel taxa can provide new perspectives on alternative physiological processes and nutrient function.

Original languageEnglish (US)
Article number26
JournalFrontiers in Zoology
Volume11
Issue number1
DOIs
StatePublished - Mar 22 2014

Fingerprint

Chamaeleonidae
immune system
carotenoid
carotenoids
lutein
lysis
agglutination
nitric oxide
plasma
wounding
repletion
animal
immunity
Chamaeleo
effect
tissue repair
nutrient availability
body mass
pigment
assimilation (physiology)

Keywords

  • Antioxidant
  • Innate immunity
  • Nitric oxide
  • Reptiles
  • Wound healing

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Ecology, Evolution, Behavior and Systematics

Cite this

The effect of carotenoid supplementation on immune system development in juvenile male veiled chameleons (Chamaeleo calyptratus). / McCartney, Kristen L.; Ligon, Russell A.; Butler, Michael W.; Denardo, Dale; McGraw, Kevin.

In: Frontiers in Zoology, Vol. 11, No. 1, 26, 22.03.2014.

Research output: Contribution to journalArticle

@article{f8d93640a87c48c089dace7f4ae36d05,
title = "The effect of carotenoid supplementation on immune system development in juvenile male veiled chameleons (Chamaeleo calyptratus)",
abstract = "Introduction: Nutrient availability, assimilation, and allocation can have important and lasting effects on the immune system development of growing animals. Though carotenoid pigments have immunostimulatory properties in many animals, relatively little is known regarding how they influence the immune system during development. Moreover, studies linking carotenoids to health at any life stage have largely been restricted to birds and mammals. We investigated the effects of carotenoid supplementation on multiple aspects of immunity in juvenile veiled chameleons (Chamaeleo calyptratus). We supplemented half of the chameleons with lutein (a xanthophyll carotenoid) for 14 weeks during development and serially measured multiple aspects of immune function, including: agglutination and lysis performance of plasma, wound healing, and plasma nitric oxide concentrations before and after wounding.Results: Though lutein supplementation effectively elevated circulating carotenoid concentrations throughout the developmental period, we found no evidence that carotenoid repletion enhanced immune function at any point. However, agglutination and lysis scores increased, while baseline nitric oxide levels decreased, as chameleons aged.Conclusions: Taken together, our results indicate that body mass and age, but not carotenoid access, may play an important role in immune performance of growing chameleons. Hence, studying well-understood physiological processes in novel taxa can provide new perspectives on alternative physiological processes and nutrient function.",
keywords = "Antioxidant, Innate immunity, Nitric oxide, Reptiles, Wound healing",
author = "McCartney, {Kristen L.} and Ligon, {Russell A.} and Butler, {Michael W.} and Dale Denardo and Kevin McGraw",
year = "2014",
month = "3",
day = "22",
doi = "10.1186/1742-9994-11-26",
language = "English (US)",
volume = "11",
journal = "Frontiers in Zoology",
issn = "1742-9994",
publisher = "BioMed Central",
number = "1",

}

TY - JOUR

T1 - The effect of carotenoid supplementation on immune system development in juvenile male veiled chameleons (Chamaeleo calyptratus)

AU - McCartney, Kristen L.

AU - Ligon, Russell A.

AU - Butler, Michael W.

AU - Denardo, Dale

AU - McGraw, Kevin

PY - 2014/3/22

Y1 - 2014/3/22

N2 - Introduction: Nutrient availability, assimilation, and allocation can have important and lasting effects on the immune system development of growing animals. Though carotenoid pigments have immunostimulatory properties in many animals, relatively little is known regarding how they influence the immune system during development. Moreover, studies linking carotenoids to health at any life stage have largely been restricted to birds and mammals. We investigated the effects of carotenoid supplementation on multiple aspects of immunity in juvenile veiled chameleons (Chamaeleo calyptratus). We supplemented half of the chameleons with lutein (a xanthophyll carotenoid) for 14 weeks during development and serially measured multiple aspects of immune function, including: agglutination and lysis performance of plasma, wound healing, and plasma nitric oxide concentrations before and after wounding.Results: Though lutein supplementation effectively elevated circulating carotenoid concentrations throughout the developmental period, we found no evidence that carotenoid repletion enhanced immune function at any point. However, agglutination and lysis scores increased, while baseline nitric oxide levels decreased, as chameleons aged.Conclusions: Taken together, our results indicate that body mass and age, but not carotenoid access, may play an important role in immune performance of growing chameleons. Hence, studying well-understood physiological processes in novel taxa can provide new perspectives on alternative physiological processes and nutrient function.

AB - Introduction: Nutrient availability, assimilation, and allocation can have important and lasting effects on the immune system development of growing animals. Though carotenoid pigments have immunostimulatory properties in many animals, relatively little is known regarding how they influence the immune system during development. Moreover, studies linking carotenoids to health at any life stage have largely been restricted to birds and mammals. We investigated the effects of carotenoid supplementation on multiple aspects of immunity in juvenile veiled chameleons (Chamaeleo calyptratus). We supplemented half of the chameleons with lutein (a xanthophyll carotenoid) for 14 weeks during development and serially measured multiple aspects of immune function, including: agglutination and lysis performance of plasma, wound healing, and plasma nitric oxide concentrations before and after wounding.Results: Though lutein supplementation effectively elevated circulating carotenoid concentrations throughout the developmental period, we found no evidence that carotenoid repletion enhanced immune function at any point. However, agglutination and lysis scores increased, while baseline nitric oxide levels decreased, as chameleons aged.Conclusions: Taken together, our results indicate that body mass and age, but not carotenoid access, may play an important role in immune performance of growing chameleons. Hence, studying well-understood physiological processes in novel taxa can provide new perspectives on alternative physiological processes and nutrient function.

KW - Antioxidant

KW - Innate immunity

KW - Nitric oxide

KW - Reptiles

KW - Wound healing

UR - http://www.scopus.com/inward/record.url?scp=84899146044&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84899146044&partnerID=8YFLogxK

U2 - 10.1186/1742-9994-11-26

DO - 10.1186/1742-9994-11-26

M3 - Article

AN - SCOPUS:84899146044

VL - 11

JO - Frontiers in Zoology

JF - Frontiers in Zoology

SN - 1742-9994

IS - 1

M1 - 26

ER -