The brown adipocyte protein CIDEA promotes lipid droplet fusion via a phosphatidic acid-binding amphipathic helix

David Barneda, Joan Planas-Iglesias, Maria L. Gaspar, Dariush Mohammadyani, Sunil Prasannan, Dirk Dormann, Gil Soo Han, Stephen A. Jesch, George M. Carman, Valerian Kagan, Malcolm G. Parker, Nicholas T. Ktistakis, Judith Klein-Seetharaman, Ann M. Dixon, Susan A. Henry, Mark Christian

Research output: Contribution to journalArticlepeer-review

60 Scopus citations

Abstract

Maintenance of energy homeostasis depends on the highly regulated storage and release of triacylglycerol primarily in adipose tissue, and excessive storage is a feature of common metabolic disorders. CIDEA is a lipid droplet (LD)-protein enriched in brown adipocytes promoting the enlargement of LDs, which are dynamic, ubiquitous organelles specialized for storing neutral lipids. We demonstrate an essential role in this process for an amphipathic helix in CIDEA, which facilitates embedding in the LD phospholipid monolayer and binds phosphatidic acid (PA). LD pairs are docked by CIDEA trans-complexes through contributions of the N-terminal domain and a C-terminal dimerization region. These complexes, enriched at the LD-LD contact site, interact with the cone-shaped phospholipid PA and likely increase phospholipid barrier permeability, promoting LD fusion by transference of lipids. This physiological process is essential in adipocyte differentiation as well as serving to facilitate the tight coupling of lipolysis and lipogenesis in activated brown fat.

Original languageEnglish (US)
Article numbere07485
JournaleLife
Volume4
Issue numberNOVEMBER2015
DOIs
StatePublished - Nov 26 2015
Externally publishedYes

ASJC Scopus subject areas

  • Neuroscience(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

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