The biogenic amine tyramine and its receptor (AmTyr1) in olfactory neuropils in the honey bee (Apis mellifera) brain

Irina Sinakevitch, Sasha M. Daskalova, Brian Smith

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

This article describes the cellular sources for tyramine and the cellular targets of tyramine via the Tyramine Receptor 1 (AmTyr1) in the olfactory learning and memory neuropils of the honey bee brain. Clusters of approximately 160 tyramine immunoreactive neurons are the source of tyraminergic fibers with small varicosities in the optic lobes, antennal lobes, lateral protocerebrum, mushroom body (calyces and gamma lobes), tritocerebrum and subesophageal ganglion (SEG). Our tyramine mapping study shows that the primary sources of tyramine in the antennal lobe and calyx of the mushroom body are from at least two Ventral Unpaired Median neurons (VUMmd and VUMmx) with cell bodies in the SEG. To reveal AmTyr1 receptors in the brain, we used newly characterized anti-AmTyr1 antibodies. Immunolocalization studies in the antennal lobe with anti-AmTyr1 antibodies showed that the AmTyr1 expression pattern is mostly in the presynaptic sites of olfactory receptor neurons (ORNs). In the mushroom body calyx, anti-AmTyr1 mapped the presynaptic sites of uniglomerular Projection Neurons (PNs) located primarily in the microglomeruli of the lip and basal ring calyx area. Release of tyramine/octopamine from VUM (md and mx) neurons in the antennal lobe and mushroom body calyx would target AmTyr1 expressed on ORN and uniglomerular PN presynaptic terminals. The presynaptic location of AmTyr1, its structural similarity with vertebrate alpha-2 adrenergic receptors, and previous pharmacological evidence suggests that it has an important role in the presynaptic inhibitory control of neurotransmitter release.

Original languageEnglish (US)
Article number77
JournalFrontiers in Systems Neuroscience
Volume11
DOIs
StatePublished - Oct 24 2017

Fingerprint

Tyramine
Biogenic Amines
Neuropil
Honey
Bees
Mushroom Bodies
Brain
Neurons
Olfactory Receptor Neurons
Ganglia
Anti-Idiotypic Antibodies
Adrenergic alpha-2 Receptors
Octopamine
Presynaptic Terminals
Lip
Neurotransmitter Agents
Vertebrates
tyramine receptor
Learning
Pharmacology

Keywords

  • Biogenic amine receptors
  • G-protein coupled receptors
  • Learning and plasticity
  • Olfactory pathways
  • Tyramine

ASJC Scopus subject areas

  • Neuroscience (miscellaneous)
  • Developmental Neuroscience
  • Cognitive Neuroscience
  • Cellular and Molecular Neuroscience

Cite this

The biogenic amine tyramine and its receptor (AmTyr1) in olfactory neuropils in the honey bee (Apis mellifera) brain. / Sinakevitch, Irina; Daskalova, Sasha M.; Smith, Brian.

In: Frontiers in Systems Neuroscience, Vol. 11, 77, 24.10.2017.

Research output: Contribution to journalArticle

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