The Arabidopsis thaliana proton transporters, AtNhx1 and Avp1, can function in cation detoxification in yeast

Roberto A. Gaxiola, Rajini Rao, Amir Sherman, Paula Grisafi, Seth L. Alper, Gerald R. Fink

Research output: Contribution to journalArticle

474 Scopus citations

Abstract

Overexpression of the Arabidopsis thaliana vacuolar H+-pyrophosphatase (AVP1) confers salt tolerance to the salt-sensitive ena1 mutant of Saccharomyces cerevisiae. Suppression of salt sensitivity requires two ion transporters, the Gef1 Cl- channel and the Nhx1 Na+/H+ exchanger. These two proteins colocalize to the prevacuolar compartment of yeast and are thought to be required for optimal acidification of this compartment. Overexpression of AtNHX1, the plant homologue of the yeast Na+/H+ exchanger, suppresses some of the mutant phenotypes of the yeast nhx1 mutant. Moreover, the level of AtNHX1 mRNA in Arabidopsis is increased in the presence of NaCl. The regulation of AtNHX1 by NaCl and the ability of the plant gene to suppress the yeast nhx1 mutant suggest that the mechanism by which cations are detoxified in yeast and plants may be similar.

Original languageEnglish (US)
Pages (from-to)1480-1485
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume96
Issue number4
DOIs
StatePublished - Feb 16 1999

ASJC Scopus subject areas

  • General

Fingerprint Dive into the research topics of 'The Arabidopsis thaliana proton transporters, AtNhx1 and Avp1, can function in cation detoxification in yeast'. Together they form a unique fingerprint.

  • Cite this