The ancient and widespread nature of the ER-mitochondria encounter structure

Jeremy G. Wideman, Ryan M.R. Gawryluk, Michael W. Gray, Joel B. Dacks

Research output: Contribution to journalArticlepeer-review

66 Scopus citations

Abstract

Mitochondria are the result of a billion years of integrative evolution, converting a once free-living bacterium to an organelle deeply linked to diverse cellular processes. One way in which mitochondria are integrated with nonendosymbiotically derived organelles is via endoplasmic reticulum (ER)-mitochondria contact sites. The ER membrane is physically tethered to the mitochondrial outer membrane by the ER-mitochondria encounter structure (ERMES). However, to date, ERMES has only ever been found in the fungal lineage. Here, we bioinformatically demonstrate that ERMES is present in lineages outside Fungi and validate this inference by mass spectrometric identification of ERMES components in Acanthamoeba castellanii mitochondria. We further demonstrate that ERMES is retained in hydrogenosome-bearing but not mitosome-bearing organisms, yielding insight into the process of reductive mitochondrial evolution. Finally, we find that the taxonomic distribution of ERMES is most consistent with rooting the eukaryotic tree between Amorphea (Animals + Fungi + Amoebozoa) + Excavata and all other eukaryotes (Diaphoratickes).

Original languageEnglish (US)
Pages (from-to)2044-2049
Number of pages6
JournalMolecular biology and evolution
Volume30
Issue number9
DOIs
StatePublished - Sep 2013
Externally publishedYes

Keywords

  • ER-mitochondria contacts
  • ERMES complex
  • hydrogenosomes and mitosomes
  • organelle evolution

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Molecular Biology
  • Genetics

Fingerprint

Dive into the research topics of 'The ancient and widespread nature of the ER-mitochondria encounter structure'. Together they form a unique fingerprint.

Cite this