The 9-kDa hydrophobic protein encoded at the 3′ end of the porcine transmissible gastroenteritis coronavirus genome is membrane-associated

Frank Y T Tung, Sushma Abraham, Manjiri Sethna, Shan Ling Hung, Phiroze Sethna, Brenda Hogue, David A. Brian

Research output: Contribution to journalArticle

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Abstract

The open reading frame potentially encoding a 78 amino acid, 9101 Da hydrophobic protein (HP) and, mapping at the 3′ end of the porcine transmissible gastroenteritis coronavirus (TGEV) genome, was shown to be expressed during virus replication. The cloned HP gene was placed in a plasmid under control of the T7 RNA polymerase promoter and in vitro translation of transcripts generated in vitro yielded a 9.1-kDa protein that was immunoprecipitable with porcine hyperimmune anti-TGEV serum. Antiserum raised in rabbits against a 31 amino acid synthetic polypeptide that represented the central hydrophilic region of HP specifically immunoprecipitated HP from TGEV-infected cells. HP was further shown to become associated with microsomal membranes during synthesis in vitro and was found to be closely associated with the endoplasmic reticulum and cell surface membranes in infected cells. The intracellular location of HP suggests that it may play a role in the membrane association of replication complexes or in virion assembly.

Original languageEnglish (US)
Pages (from-to)676-683
Number of pages8
JournalVirology
Volume186
Issue number2
DOIs
StatePublished - 1992
Externally publishedYes

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Transmissible Gastroenteritis of Swine
Coronavirus
Genome
Membranes
Proteins
Gastroenteritis
Amino Acids
Virus Replication
Endoplasmic Reticulum
Virion
Open Reading Frames
Immune Sera
Plasmids
Swine
Cell Membrane
Rabbits

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

Cite this

The 9-kDa hydrophobic protein encoded at the 3′ end of the porcine transmissible gastroenteritis coronavirus genome is membrane-associated. / Tung, Frank Y T; Abraham, Sushma; Sethna, Manjiri; Hung, Shan Ling; Sethna, Phiroze; Hogue, Brenda; Brian, David A.

In: Virology, Vol. 186, No. 2, 1992, p. 676-683.

Research output: Contribution to journalArticle

Tung, Frank Y T ; Abraham, Sushma ; Sethna, Manjiri ; Hung, Shan Ling ; Sethna, Phiroze ; Hogue, Brenda ; Brian, David A. / The 9-kDa hydrophobic protein encoded at the 3′ end of the porcine transmissible gastroenteritis coronavirus genome is membrane-associated. In: Virology. 1992 ; Vol. 186, No. 2. pp. 676-683.
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