The 2 micron plasmid purloins the yeast cohesin complex: A mechanism for coupling plasmid partitioning and chromosome segregation?

Shwetal Mehta, Xian Mei Yang, Clarence S. Chan, Melanie J. Dobson, Makkuni Jayaram, Soundarapandian Velmurugan

Research output: Contribution to journalArticlepeer-review

50 Scopus citations

Abstract

The yeast 2 micron plasmid achieves high fidelity segregation by coupling its partitioning pathway to that of the chromosomes. Mutations affecting distinct steps of chromosome segregation cause the plasmid to missegregate in tandem with the chromosomes. In the absence of the plasmid stability system, consisting of the Rep1 and Rep2 proteins and the STB DNA, plasmid and chromosome segregations are uncoupled. The Rep proteins, acting in concert, recruit the yeast cohesin complex to the STB locus. The periodicity of cohesin association and dissociation is nearly identical for the plasmid and the chromosomes. The timely disassembly of cohesin is a prerequisite for plasmid segregation. Cohesin-mediated pairing and unpairing likely provides a counting mechanism for evenly partitioning plasmids either in association with or independently of the chromosomes.

Original languageEnglish (US)
Pages (from-to)625-637
Number of pages13
JournalJournal of Cell Biology
Volume158
Issue number4
DOIs
StatePublished - Aug 19 2002
Externally publishedYes

Keywords

  • Chromatin immunoprecipitation
  • Cohesin recruitment
  • Monohybrid assay
  • Nondegradable Mcd 1 p
  • Yeast plasmid segregation

ASJC Scopus subject areas

  • Cell Biology

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