TY - JOUR
T1 - The 2 micron plasmid purloins the yeast cohesin complex
T2 - A mechanism for coupling plasmid partitioning and chromosome segregation?
AU - Mehta, Shwetal
AU - Yang, Xian Mei
AU - Chan, Clarence S.
AU - Dobson, Melanie J.
AU - Jayaram, Makkuni
AU - Velmurugan, Soundarapandian
PY - 2002/8/19
Y1 - 2002/8/19
N2 - The yeast 2 micron plasmid achieves high fidelity segregation by coupling its partitioning pathway to that of the chromosomes. Mutations affecting distinct steps of chromosome segregation cause the plasmid to missegregate in tandem with the chromosomes. In the absence of the plasmid stability system, consisting of the Rep1 and Rep2 proteins and the STB DNA, plasmid and chromosome segregations are uncoupled. The Rep proteins, acting in concert, recruit the yeast cohesin complex to the STB locus. The periodicity of cohesin association and dissociation is nearly identical for the plasmid and the chromosomes. The timely disassembly of cohesin is a prerequisite for plasmid segregation. Cohesin-mediated pairing and unpairing likely provides a counting mechanism for evenly partitioning plasmids either in association with or independently of the chromosomes.
AB - The yeast 2 micron plasmid achieves high fidelity segregation by coupling its partitioning pathway to that of the chromosomes. Mutations affecting distinct steps of chromosome segregation cause the plasmid to missegregate in tandem with the chromosomes. In the absence of the plasmid stability system, consisting of the Rep1 and Rep2 proteins and the STB DNA, plasmid and chromosome segregations are uncoupled. The Rep proteins, acting in concert, recruit the yeast cohesin complex to the STB locus. The periodicity of cohesin association and dissociation is nearly identical for the plasmid and the chromosomes. The timely disassembly of cohesin is a prerequisite for plasmid segregation. Cohesin-mediated pairing and unpairing likely provides a counting mechanism for evenly partitioning plasmids either in association with or independently of the chromosomes.
KW - Chromatin immunoprecipitation
KW - Cohesin recruitment
KW - Monohybrid assay
KW - Nondegradable Mcd 1 p
KW - Yeast plasmid segregation
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U2 - 10.1083/jcb.200204136
DO - 10.1083/jcb.200204136
M3 - Article
C2 - 12177044
AN - SCOPUS:0037135985
SN - 0021-9525
VL - 158
SP - 625
EP - 637
JO - Journal of Cell Biology
JF - Journal of Cell Biology
IS - 4
ER -