Targeted cleavage of RNA using eukaryotic ribonuclease P and external guide sequence

Yan Yuan, Cecilia Guerrier-Takada, Sidney Altman, Fenyong Liu

Research output: Contribution to journalArticle

Abstract

It has been discovered that any RNA can be targeted for cleavage by RNAase P from eukaryotic cells, for example, human RNAase P, using a suitably designed oligoribonucleotide (external guide sequence, or EGS) to form a hybrid with the target RNA, thereby creating a substrate for cleavage by RNAase P in vitro. The EGS hydrogen bonds to the targeted RNA to form a partial tRNA like structure including the aminoacyl acceptor stem, the T stem and loop, and part of the D stem. The most efficient EGS with human RNAase P is the EGS in which the anticodon stem and loop was deleted. Modifications can also be made within the T-loop. Methods are also disclosed to randomly select and to express a suitable EGS in vivo to make a selected RNA a target for cleavage by the host cell RNAase P, thus preventing expression of the function of the target RNA. The methods and compositions should be useful to prevent the expression of disease-causing genes in vivo.

Original languageEnglish (US)
Pages (from-to)681-682
Number of pages2
JournalBiotechnology Advances
Volume15
Issue number3-4
StatePublished - 1997
Externally publishedYes

Fingerprint

Ribonuclease P
RNA Cleavage
RNA
Oligoribonucleotides
Anticodon
Eukaryotic Cells
Transfer RNA
Hydrogen
Hydrogen bonds
Genes
Ribonucleases
Substrates
Chemical analysis

ASJC Scopus subject areas

  • Biotechnology

Cite this

Targeted cleavage of RNA using eukaryotic ribonuclease P and external guide sequence. / Yuan, Yan; Guerrier-Takada, Cecilia; Altman, Sidney; Liu, Fenyong.

In: Biotechnology Advances, Vol. 15, No. 3-4, 1997, p. 681-682.

Research output: Contribution to journalArticle

Yuan, Y, Guerrier-Takada, C, Altman, S & Liu, F 1997, 'Targeted cleavage of RNA using eukaryotic ribonuclease P and external guide sequence', Biotechnology Advances, vol. 15, no. 3-4, pp. 681-682.
Yuan, Yan ; Guerrier-Takada, Cecilia ; Altman, Sidney ; Liu, Fenyong. / Targeted cleavage of RNA using eukaryotic ribonuclease P and external guide sequence. In: Biotechnology Advances. 1997 ; Vol. 15, No. 3-4. pp. 681-682.
@article{64236f0440ba4f72ae7dab1d15d4e38d,
title = "Targeted cleavage of RNA using eukaryotic ribonuclease P and external guide sequence",
abstract = "It has been discovered that any RNA can be targeted for cleavage by RNAase P from eukaryotic cells, for example, human RNAase P, using a suitably designed oligoribonucleotide (external guide sequence, or EGS) to form a hybrid with the target RNA, thereby creating a substrate for cleavage by RNAase P in vitro. The EGS hydrogen bonds to the targeted RNA to form a partial tRNA like structure including the aminoacyl acceptor stem, the T stem and loop, and part of the D stem. The most efficient EGS with human RNAase P is the EGS in which the anticodon stem and loop was deleted. Modifications can also be made within the T-loop. Methods are also disclosed to randomly select and to express a suitable EGS in vivo to make a selected RNA a target for cleavage by the host cell RNAase P, thus preventing expression of the function of the target RNA. The methods and compositions should be useful to prevent the expression of disease-causing genes in vivo.",
author = "Yan Yuan and Cecilia Guerrier-Takada and Sidney Altman and Fenyong Liu",
year = "1997",
language = "English (US)",
volume = "15",
pages = "681--682",
journal = "Biotechnology Advances",
issn = "0734-9750",
publisher = "Elsevier Inc.",
number = "3-4",

}

TY - JOUR

T1 - Targeted cleavage of RNA using eukaryotic ribonuclease P and external guide sequence

AU - Yuan, Yan

AU - Guerrier-Takada, Cecilia

AU - Altman, Sidney

AU - Liu, Fenyong

PY - 1997

Y1 - 1997

N2 - It has been discovered that any RNA can be targeted for cleavage by RNAase P from eukaryotic cells, for example, human RNAase P, using a suitably designed oligoribonucleotide (external guide sequence, or EGS) to form a hybrid with the target RNA, thereby creating a substrate for cleavage by RNAase P in vitro. The EGS hydrogen bonds to the targeted RNA to form a partial tRNA like structure including the aminoacyl acceptor stem, the T stem and loop, and part of the D stem. The most efficient EGS with human RNAase P is the EGS in which the anticodon stem and loop was deleted. Modifications can also be made within the T-loop. Methods are also disclosed to randomly select and to express a suitable EGS in vivo to make a selected RNA a target for cleavage by the host cell RNAase P, thus preventing expression of the function of the target RNA. The methods and compositions should be useful to prevent the expression of disease-causing genes in vivo.

AB - It has been discovered that any RNA can be targeted for cleavage by RNAase P from eukaryotic cells, for example, human RNAase P, using a suitably designed oligoribonucleotide (external guide sequence, or EGS) to form a hybrid with the target RNA, thereby creating a substrate for cleavage by RNAase P in vitro. The EGS hydrogen bonds to the targeted RNA to form a partial tRNA like structure including the aminoacyl acceptor stem, the T stem and loop, and part of the D stem. The most efficient EGS with human RNAase P is the EGS in which the anticodon stem and loop was deleted. Modifications can also be made within the T-loop. Methods are also disclosed to randomly select and to express a suitable EGS in vivo to make a selected RNA a target for cleavage by the host cell RNAase P, thus preventing expression of the function of the target RNA. The methods and compositions should be useful to prevent the expression of disease-causing genes in vivo.

UR - http://www.scopus.com/inward/record.url?scp=0030662896&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030662896&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:0030662896

VL - 15

SP - 681

EP - 682

JO - Biotechnology Advances

JF - Biotechnology Advances

SN - 0734-9750

IS - 3-4

ER -