Synthesis of peptide-oligonucleotide conjugates using a heterobifunctional crosslinker

Berea A R Williams, John C. Chaput

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Peptide-oligonucleotide conjugates (POCs) are molecular chimeras composed of a nucleic acid moiety covalently attached to a polypeptide moiety. POCs have been used in numerous applications from therapeutics to nanotechnology, and most recently as combinatorial agents in the assembly of bivalent protein affinity reagents. This unit describes the synthesis and purification of POC molecules using the heterobifunctional crosslinking reagent succinimidyl-4-(N- maleimidomethyl)cyclohexane-1-carboxylate (SMCC), which enables amine-modified oligonucleotides to become covalently linked to cysteine-modified polypeptides. This solution-based protocol consists of a two-step synthesis followed by a single purification step.

Original languageEnglish (US)
JournalCurrent Protocols in Nucleic Acid Chemistry
Issue numberSUPPL. 42
DOIs
StatePublished - 2010

Fingerprint

Oligonucleotides
Peptides
Purification
Cross-Linking Reagents
Nanotechnology
Nucleic Acids
Amines
Cysteine
Molecules
Proteins
Therapeutics

Keywords

  • antisense
  • DNA nanotechnology
  • fragment conjugation
  • nanodisplay
  • peptide-oligonucleotide conjugate
  • succinimidyl-4-(N-maleimidomethyl) cyclohexane-1-carboxylate (SMCC)
  • synbodies

ASJC Scopus subject areas

  • Biochemistry
  • Organic Chemistry

Cite this

Synthesis of peptide-oligonucleotide conjugates using a heterobifunctional crosslinker. / Williams, Berea A R; Chaput, John C.

In: Current Protocols in Nucleic Acid Chemistry, No. SUPPL. 42, 2010.

Research output: Contribution to journalArticle

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