Surface plasmon resonance-enabled mass spectrometry arrays

Dobrin Nedelkov, Kemmons A. Tubbs, Randall W. Nelson

Research output: Contribution to journalArticle

24 Scopus citations

Abstract

Biosensors that utilize surface plasmon resonance (SPR) as a method of detection of protein interactions can be used for selective separation of proteins prior to MS analysis. The combination of SPR and MS results in a unique multiplexed detection technology capable of both quantitative and qualitative protein analysis. To further the development of a high-throughput SPR-MS approach, the possibility of arraying binding ligands on SPR chips for affinity capture of proteins and their MS analysis was explored. Antibodies to β-2-microglobulin, cystatin C, transferrin, and insulin-like growth factors I and II were arrayed on a large number of SPR chips. Human plasma samples were injected over the antibody array chips in an SPR Biosensor, after which on-chip MS analysis was performed to detect the bound proteins. Signals from the targeted proteins were observed for each antibody-derivatized chip, indicating successful antibody immobilization and protein capture. The SPR-MS arrays are robust, highly reproducible, and are capable of high-throughput analysis.

Original languageEnglish (US)
Pages (from-to)3671-3675
Number of pages5
JournalElectrophoresis
Volume27
Issue number18
DOIs
StatePublished - Sep 2006

Keywords

  • Arrays
  • Mass spectrometry
  • Plasma
  • Protein
  • Surface plasmon resonance

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Clinical Biochemistry

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    Nedelkov, D., Tubbs, K. A., & Nelson, R. W. (2006). Surface plasmon resonance-enabled mass spectrometry arrays. Electrophoresis, 27(18), 3671-3675. https://doi.org/10.1002/elps.200600065