Surface plasmon resonance-enabled mass spectrometry arrays

Dobrin Nedelkov, Kemmons A. Tubbs, Randall W. Nelson

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Biosensors that utilize surface plasmon resonance (SPR) as a method of detection of protein interactions can be used for selective separation of proteins prior to MS analysis. The combination of SPR and MS results in a unique multiplexed detection technology capable of both quantitative and qualitative protein analysis. To further the development of a high-throughput SPR-MS approach, the possibility of arraying binding ligands on SPR chips for affinity capture of proteins and their MS analysis was explored. Antibodies to β-2-microglobulin, cystatin C, transferrin, and insulin-like growth factors I and II were arrayed on a large number of SPR chips. Human plasma samples were injected over the antibody array chips in an SPR Biosensor, after which on-chip MS analysis was performed to detect the bound proteins. Signals from the targeted proteins were observed for each antibody-derivatized chip, indicating successful antibody immobilization and protein capture. The SPR-MS arrays are robust, highly reproducible, and are capable of high-throughput analysis.

Original languageEnglish (US)
Pages (from-to)3671-3675
Number of pages5
JournalElectrophoresis
Volume27
Issue number18
DOIs
StatePublished - Sep 2006

Fingerprint

Surface Plasmon Resonance
Surface plasmon resonance
Mass spectrometry
Mass Spectrometry
Proteins
Antibodies
Biosensing Techniques
Biosensors
Throughput
Plasma (human)
Cystatin C
Insulin-Like Growth Factor II
Transferrin
Insulin-Like Growth Factor I
Immobilization
Ligands
Technology

Keywords

  • Arrays
  • Mass spectrometry
  • Plasma
  • Protein
  • Surface plasmon resonance

ASJC Scopus subject areas

  • Clinical Biochemistry

Cite this

Nedelkov, D., Tubbs, K. A., & Nelson, R. W. (2006). Surface plasmon resonance-enabled mass spectrometry arrays. Electrophoresis, 27(18), 3671-3675. https://doi.org/10.1002/elps.200600065

Surface plasmon resonance-enabled mass spectrometry arrays. / Nedelkov, Dobrin; Tubbs, Kemmons A.; Nelson, Randall W.

In: Electrophoresis, Vol. 27, No. 18, 09.2006, p. 3671-3675.

Research output: Contribution to journalArticle

Nedelkov, D, Tubbs, KA & Nelson, RW 2006, 'Surface plasmon resonance-enabled mass spectrometry arrays', Electrophoresis, vol. 27, no. 18, pp. 3671-3675. https://doi.org/10.1002/elps.200600065
Nedelkov, Dobrin ; Tubbs, Kemmons A. ; Nelson, Randall W. / Surface plasmon resonance-enabled mass spectrometry arrays. In: Electrophoresis. 2006 ; Vol. 27, No. 18. pp. 3671-3675.
@article{b51a4dcc4b294990b903ba0fc7815b6f,
title = "Surface plasmon resonance-enabled mass spectrometry arrays",
abstract = "Biosensors that utilize surface plasmon resonance (SPR) as a method of detection of protein interactions can be used for selective separation of proteins prior to MS analysis. The combination of SPR and MS results in a unique multiplexed detection technology capable of both quantitative and qualitative protein analysis. To further the development of a high-throughput SPR-MS approach, the possibility of arraying binding ligands on SPR chips for affinity capture of proteins and their MS analysis was explored. Antibodies to β-2-microglobulin, cystatin C, transferrin, and insulin-like growth factors I and II were arrayed on a large number of SPR chips. Human plasma samples were injected over the antibody array chips in an SPR Biosensor, after which on-chip MS analysis was performed to detect the bound proteins. Signals from the targeted proteins were observed for each antibody-derivatized chip, indicating successful antibody immobilization and protein capture. The SPR-MS arrays are robust, highly reproducible, and are capable of high-throughput analysis.",
keywords = "Arrays, Mass spectrometry, Plasma, Protein, Surface plasmon resonance",
author = "Dobrin Nedelkov and Tubbs, {Kemmons A.} and Nelson, {Randall W.}",
year = "2006",
month = "9",
doi = "10.1002/elps.200600065",
language = "English (US)",
volume = "27",
pages = "3671--3675",
journal = "Electrophoresis",
issn = "0173-0835",
publisher = "Wiley-VCH Verlag",
number = "18",

}

TY - JOUR

T1 - Surface plasmon resonance-enabled mass spectrometry arrays

AU - Nedelkov, Dobrin

AU - Tubbs, Kemmons A.

AU - Nelson, Randall W.

PY - 2006/9

Y1 - 2006/9

N2 - Biosensors that utilize surface plasmon resonance (SPR) as a method of detection of protein interactions can be used for selective separation of proteins prior to MS analysis. The combination of SPR and MS results in a unique multiplexed detection technology capable of both quantitative and qualitative protein analysis. To further the development of a high-throughput SPR-MS approach, the possibility of arraying binding ligands on SPR chips for affinity capture of proteins and their MS analysis was explored. Antibodies to β-2-microglobulin, cystatin C, transferrin, and insulin-like growth factors I and II were arrayed on a large number of SPR chips. Human plasma samples were injected over the antibody array chips in an SPR Biosensor, after which on-chip MS analysis was performed to detect the bound proteins. Signals from the targeted proteins were observed for each antibody-derivatized chip, indicating successful antibody immobilization and protein capture. The SPR-MS arrays are robust, highly reproducible, and are capable of high-throughput analysis.

AB - Biosensors that utilize surface plasmon resonance (SPR) as a method of detection of protein interactions can be used for selective separation of proteins prior to MS analysis. The combination of SPR and MS results in a unique multiplexed detection technology capable of both quantitative and qualitative protein analysis. To further the development of a high-throughput SPR-MS approach, the possibility of arraying binding ligands on SPR chips for affinity capture of proteins and their MS analysis was explored. Antibodies to β-2-microglobulin, cystatin C, transferrin, and insulin-like growth factors I and II were arrayed on a large number of SPR chips. Human plasma samples were injected over the antibody array chips in an SPR Biosensor, after which on-chip MS analysis was performed to detect the bound proteins. Signals from the targeted proteins were observed for each antibody-derivatized chip, indicating successful antibody immobilization and protein capture. The SPR-MS arrays are robust, highly reproducible, and are capable of high-throughput analysis.

KW - Arrays

KW - Mass spectrometry

KW - Plasma

KW - Protein

KW - Surface plasmon resonance

UR - http://www.scopus.com/inward/record.url?scp=33749454392&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33749454392&partnerID=8YFLogxK

U2 - 10.1002/elps.200600065

DO - 10.1002/elps.200600065

M3 - Article

C2 - 16915566

AN - SCOPUS:33749454392

VL - 27

SP - 3671

EP - 3675

JO - Electrophoresis

JF - Electrophoresis

SN - 0173-0835

IS - 18

ER -