Abstract
Biosensors that utilize surface plasmon resonance (SPR) as a method of detection of protein interactions can be used for selective separation of proteins prior to MS analysis. The combination of SPR and MS results in a unique multiplexed detection technology capable of both quantitative and qualitative protein analysis. To further the development of a high-throughput SPR-MS approach, the possibility of arraying binding ligands on SPR chips for affinity capture of proteins and their MS analysis was explored. Antibodies to β-2-microglobulin, cystatin C, transferrin, and insulin-like growth factors I and II were arrayed on a large number of SPR chips. Human plasma samples were injected over the antibody array chips in an SPR Biosensor, after which on-chip MS analysis was performed to detect the bound proteins. Signals from the targeted proteins were observed for each antibody-derivatized chip, indicating successful antibody immobilization and protein capture. The SPR-MS arrays are robust, highly reproducible, and are capable of high-throughput analysis.
Original language | English (US) |
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Pages (from-to) | 3671-3675 |
Number of pages | 5 |
Journal | Electrophoresis |
Volume | 27 |
Issue number | 18 |
DOIs | |
State | Published - Sep 2006 |
Keywords
- Arrays
- Mass spectrometry
- Plasma
- Protein
- Surface plasmon resonance
ASJC Scopus subject areas
- Analytical Chemistry
- Biochemistry
- Clinical Biochemistry