Structure of phenoxazinone synthase from Streptomyces antibioticus reveals a new type 2 copper center

Alex W. Smith, Ana Camara-Artigas, Meitian Wang, James Allen, Wilson A. Francisco

Research output: Contribution to journalArticle

85 Scopus citations

Abstract

The multicopper oxidase phenoxazinone synthase (PHS) catalyzes the penultimate step in the biosynthesis of the antibiotic actinomycin D by Streptomyces antibioticus. PHS exists in two oligomeric forms: a dimeric form and a hexameric form, with older actinomycin-producing cultures containing predominately the hexameric form. The structure of hexameric PHS has been determined using X-ray diffraction to a resolution limit of 2.30 Å and is found to contain several unexpected and distinctive features. The structure forms a hexameric ring that is centered on a pseudo 6-fold axis and has an outer diameter of 185 Å with a large central cavity that has a diameter of 50 Å. This hexameric structure is stabilized by a long loop connecting two domains; bound to this long loop is a fifth copper atom that is present as a type 2 copper. This copper atom is not present in any other multicopper oxidase, and its presence appears to stabilize the hexameric structure.

Original languageEnglish (US)
Pages (from-to)4378-4387
Number of pages10
JournalBiochemistry
Volume45
Issue number14
DOIs
StatePublished - Apr 11 2006

ASJC Scopus subject areas

  • Biochemistry

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