Structural and electron transfer properties of cytochrome c adsorbed on graphite electrode studied by in situ tapping mode AFM

S. Boussaad, Nongjian Tao, R. Arechabaleta

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

We have investigated the adsorption of redox protein, cytochrome c, on graphite electrode from phosphate buffer by in situ tapping mode AFM. The protein adsorbs spontaneously onto the electrode that is covered with an adsorbed phosphate layer and forms a uniform monolayer. The adsorbed protein exhibits a reversible electron transfer at 0.17 V (Ag/AgCl) once the electrode has been increased to 0.75 V. We suggest that the adsorbed protein retains the folded conformation, and increasing the potential induces the protein to reorient its positively charged lysine residues towards the phosphate layer which promotes the electron transfer.

Original languageEnglish (US)
Pages (from-to)397-403
Number of pages7
JournalChemical Physics Letters
Volume280
Issue number3-4
StatePublished - Dec 5 1997
Externally publishedYes

Fingerprint

Graphite electrodes
cytochromes
Cytochromes c
electron transfer
graphite
atomic force microscopy
proteins
electrodes
Electrons
phosphates
Phosphates
Proteins
Electrodes
lysine
Lysine
Conformations
Monolayers
Buffers
buffers
Adsorption

ASJC Scopus subject areas

  • Physical and Theoretical Chemistry
  • Spectroscopy
  • Atomic and Molecular Physics, and Optics

Cite this

Structural and electron transfer properties of cytochrome c adsorbed on graphite electrode studied by in situ tapping mode AFM. / Boussaad, S.; Tao, Nongjian; Arechabaleta, R.

In: Chemical Physics Letters, Vol. 280, No. 3-4, 05.12.1997, p. 397-403.

Research output: Contribution to journalArticle

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