Abstract

Scaffolded DNA origami, a method to create self-assembled nanostructures with spatially addressable features, has recently been used to develop water-soluble molecular chips for label-free RNA detection, platforms for deterministic protein positioning, and single molecule reaction observatories. These applications highlight the possibility of exploiting the unique properties and biocompatibility of DNA nanostructures in live, cellular systems. Herein, we assembled several DNA origami nanostructures of differing shape, size and probes, and investigated their interaction with lysate obtained from various normal and cancerous cell lines. We separated and analyzed the origami-lysate mixtures using agarose gel electrophoresis and recovered the DNA structures for functional assay and subsequent microscopic examination. Our results demonstrate that DNA origami nanostructures are stable in cell lysate and can be easily separated from lysate mixtures, in contrast to natural, single- and double-stranded DNA. Atomic force microscope (AFM) and transmission electron microscope (TEM) images show that the DNA origami structures are fully intact after separation from cell lysates and hybridize to their targets, verifying the superior structural integrity and functionality of self-assembled DNA origami nanostructures relative to conventional oligonucleotides. The stability and functionality of DNA origami structures in cell lysate validate their use for biological applications, for example, as programmable molecular rafts or disease detection platforms.

Original languageEnglish (US)
Pages (from-to)1477-1482
Number of pages6
JournalNano Letters
Volume11
Issue number4
DOIs
StatePublished - Apr 13 2011

Fingerprint

DNA
deoxyribonucleic acid
cells
Nanostructures
platforms
rafts
oligonucleotides
Oligonucleotides
Single-Stranded DNA
biocompatibility
Structural integrity
Observatories
electrophoresis
Electrophoresis
RNA
Biocompatibility
cultured cells
Sepharose
integrity
positioning

Keywords

  • biocompatibility
  • DNA origami
  • self-assembly
  • structural DNA nanotechnology

ASJC Scopus subject areas

  • Condensed Matter Physics
  • Bioengineering
  • Chemistry(all)
  • Materials Science(all)
  • Mechanical Engineering

Cite this

Mei, Q., Wei, X., Su, F., Liu, Y., Youngbull, C., Johnson, R., ... Meldrum, D. (2011). Stability of DNA origami nanoarrays in cell lysate. Nano Letters, 11(4), 1477-1482. https://doi.org/10.1021/nl1040836

Stability of DNA origami nanoarrays in cell lysate. / Mei, Qian; Wei, Xixi; Su, Fengyu; Liu, Yan; Youngbull, Cody; Johnson, Roger; Lindsay, Stuart; Yan, Hao; Meldrum, Deirdre.

In: Nano Letters, Vol. 11, No. 4, 13.04.2011, p. 1477-1482.

Research output: Contribution to journalArticle

Mei, Q, Wei, X, Su, F, Liu, Y, Youngbull, C, Johnson, R, Lindsay, S, Yan, H & Meldrum, D 2011, 'Stability of DNA origami nanoarrays in cell lysate', Nano Letters, vol. 11, no. 4, pp. 1477-1482. https://doi.org/10.1021/nl1040836
Mei Q, Wei X, Su F, Liu Y, Youngbull C, Johnson R et al. Stability of DNA origami nanoarrays in cell lysate. Nano Letters. 2011 Apr 13;11(4):1477-1482. https://doi.org/10.1021/nl1040836
Mei, Qian ; Wei, Xixi ; Su, Fengyu ; Liu, Yan ; Youngbull, Cody ; Johnson, Roger ; Lindsay, Stuart ; Yan, Hao ; Meldrum, Deirdre. / Stability of DNA origami nanoarrays in cell lysate. In: Nano Letters. 2011 ; Vol. 11, No. 4. pp. 1477-1482.
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