Spleen lymphocyte proteome analysis in bronchial asthma of wistar rats

Shan Yu Li, Jia Fan, Jing Hui Xi, Hai Yu Zhang, Hong Wei Du, Xin Zhou, Xu Wang, Bo Ning, Lan Ying Liu, Dong Yun Hao

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

To study the molecular mechanisms of asthma, the bronchial asthma model was established with OVA-sensitized Wistar rats. The differentially expressed spleen lymphocyte proteomes between asthmatic rats and normal rats were profiled and the proteins were identified via two-dimensional electrophoresis and MALDI-TOF-MS. The results reveal that the expression of some protein genes was up-regulated in asthmatic rats in comparison with the normal rats. These includes hnRNPK, involving the transcriptional regulation of collagen proteins, and cell structural protein Fibrinogen. However, the energetic metabolism-relating protein such as CoQ10 and mitochondrial inner membrane protein were exhibited to be down-regulated. Further analysis using bioinformatic tools suggests that the above-mentioned proteins are likely important components involving the airway chronic inflammation, airway hyper-responsiveness and airway remodeling of asthmatic rats.

Original languageEnglish (US)
Pages (from-to)1696-1700
Number of pages5
JournalGaodeng Xuexiao Huaxue Xuebao/Chemical Journal of Chinese Universities
Volume28
Issue number9
StatePublished - Sep 2007

Keywords

  • 2-DE/MS
  • Bronchial asthma
  • Differential proteome
  • Lymphocyte

ASJC Scopus subject areas

  • Chemistry(all)

Fingerprint Dive into the research topics of 'Spleen lymphocyte proteome analysis in bronchial asthma of wistar rats'. Together they form a unique fingerprint.

Cite this