Site-specific phosphorylation of protein phosphatase 1 regulatory subunit 12A stimulated or suppressed by insulin

Alex Chao, Xiangmin Zhang, Danjun Ma, Paul Langlais, Moulun Luo, Lawrence J. Mandarino, Morgan Zingsheim, Kimberly Pham, James Dillon, Zhengping Yi

    Research output: Contribution to journalArticle

    10 Citations (Scopus)

    Abstract

    Protein phosphatase 1 (PP1) is one of the major phosphatases responsible for protein dephosphorylation in eukaryotes. So far, only few specific phosphorylation sites of PP1 regulatory subunit 12A (PPP1R12A) have been shown to regulate the PP1 activity. The effect of insulin on PPP1R12A phosphorylation is largely unknown. Utilizing a mass spectrometry based phosphorylation identification and quantification approach, we identified 21 PPP1R12A phosphorylation sites (7 novel sites, including Ser20, Thr22, Thr453, Ser478, Thr671, Ser678, and Ser680) and quantified 16 of them under basal and insulin stimulated conditions in hamster ovary cells overexpressing the insulin receptor (CHO/IR), an insulin sensitive cell model. Insulin stimulated the phosphorylation of PPP1R12A significantly at Ser477, Ser478, Ser507, Ser668, and Ser695, while simultaneously suppressing the phosphorylation of PPP1R12A at Ser509 (more than 2-fold increase or decrease compared to basal). Our data demonstrate that PPP1R12A undergoes insulin stimulated/suppressed phosphorylation, suggesting that PPP1R12A phosphorylation may play a role in insulin signal transduction. The novel PPP1R12A phosphorylation sites as well as the new insulin-responsive phosphorylation sites of PPP1R12A in CHO/IR cells provide targets for investigation of the regulation of PPP1R12A and the PPP1R12A-PP1cδ complex in insulin action and other signaling pathways in other cell models, animal models, and humans.

    Original languageEnglish (US)
    Pages (from-to)3342-3350
    Number of pages9
    JournalJournal of Proteomics
    Volume75
    Issue number11
    DOIs
    StatePublished - Jun 18 2012

    Fingerprint

    Protein Phosphatase 1
    Phosphorylation
    Insulin

    Keywords

    • HPLC-ESI-MS/MS
    • Phosphorylation
    • PPP1R12A
    • Quantification

    ASJC Scopus subject areas

    • Biochemistry
    • Biophysics

    Cite this

    Site-specific phosphorylation of protein phosphatase 1 regulatory subunit 12A stimulated or suppressed by insulin. / Chao, Alex; Zhang, Xiangmin; Ma, Danjun; Langlais, Paul; Luo, Moulun; Mandarino, Lawrence J.; Zingsheim, Morgan; Pham, Kimberly; Dillon, James; Yi, Zhengping.

    In: Journal of Proteomics, Vol. 75, No. 11, 18.06.2012, p. 3342-3350.

    Research output: Contribution to journalArticle

    Chao, A, Zhang, X, Ma, D, Langlais, P, Luo, M, Mandarino, LJ, Zingsheim, M, Pham, K, Dillon, J & Yi, Z 2012, 'Site-specific phosphorylation of protein phosphatase 1 regulatory subunit 12A stimulated or suppressed by insulin', Journal of Proteomics, vol. 75, no. 11, pp. 3342-3350. https://doi.org/10.1016/j.jprot.2012.03.043
    Chao, Alex ; Zhang, Xiangmin ; Ma, Danjun ; Langlais, Paul ; Luo, Moulun ; Mandarino, Lawrence J. ; Zingsheim, Morgan ; Pham, Kimberly ; Dillon, James ; Yi, Zhengping. / Site-specific phosphorylation of protein phosphatase 1 regulatory subunit 12A stimulated or suppressed by insulin. In: Journal of Proteomics. 2012 ; Vol. 75, No. 11. pp. 3342-3350.
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    AU - Chao, Alex

    AU - Zhang, Xiangmin

    AU - Ma, Danjun

    AU - Langlais, Paul

    AU - Luo, Moulun

    AU - Mandarino, Lawrence J.

    AU - Zingsheim, Morgan

    AU - Pham, Kimberly

    AU - Dillon, James

    AU - Yi, Zhengping

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    N2 - Protein phosphatase 1 (PP1) is one of the major phosphatases responsible for protein dephosphorylation in eukaryotes. So far, only few specific phosphorylation sites of PP1 regulatory subunit 12A (PPP1R12A) have been shown to regulate the PP1 activity. The effect of insulin on PPP1R12A phosphorylation is largely unknown. Utilizing a mass spectrometry based phosphorylation identification and quantification approach, we identified 21 PPP1R12A phosphorylation sites (7 novel sites, including Ser20, Thr22, Thr453, Ser478, Thr671, Ser678, and Ser680) and quantified 16 of them under basal and insulin stimulated conditions in hamster ovary cells overexpressing the insulin receptor (CHO/IR), an insulin sensitive cell model. Insulin stimulated the phosphorylation of PPP1R12A significantly at Ser477, Ser478, Ser507, Ser668, and Ser695, while simultaneously suppressing the phosphorylation of PPP1R12A at Ser509 (more than 2-fold increase or decrease compared to basal). Our data demonstrate that PPP1R12A undergoes insulin stimulated/suppressed phosphorylation, suggesting that PPP1R12A phosphorylation may play a role in insulin signal transduction. The novel PPP1R12A phosphorylation sites as well as the new insulin-responsive phosphorylation sites of PPP1R12A in CHO/IR cells provide targets for investigation of the regulation of PPP1R12A and the PPP1R12A-PP1cδ complex in insulin action and other signaling pathways in other cell models, animal models, and humans.

    AB - Protein phosphatase 1 (PP1) is one of the major phosphatases responsible for protein dephosphorylation in eukaryotes. So far, only few specific phosphorylation sites of PP1 regulatory subunit 12A (PPP1R12A) have been shown to regulate the PP1 activity. The effect of insulin on PPP1R12A phosphorylation is largely unknown. Utilizing a mass spectrometry based phosphorylation identification and quantification approach, we identified 21 PPP1R12A phosphorylation sites (7 novel sites, including Ser20, Thr22, Thr453, Ser478, Thr671, Ser678, and Ser680) and quantified 16 of them under basal and insulin stimulated conditions in hamster ovary cells overexpressing the insulin receptor (CHO/IR), an insulin sensitive cell model. Insulin stimulated the phosphorylation of PPP1R12A significantly at Ser477, Ser478, Ser507, Ser668, and Ser695, while simultaneously suppressing the phosphorylation of PPP1R12A at Ser509 (more than 2-fold increase or decrease compared to basal). Our data demonstrate that PPP1R12A undergoes insulin stimulated/suppressed phosphorylation, suggesting that PPP1R12A phosphorylation may play a role in insulin signal transduction. The novel PPP1R12A phosphorylation sites as well as the new insulin-responsive phosphorylation sites of PPP1R12A in CHO/IR cells provide targets for investigation of the regulation of PPP1R12A and the PPP1R12A-PP1cδ complex in insulin action and other signaling pathways in other cell models, animal models, and humans.

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    KW - Quantification

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