Site-specific incorporation of glycosylated serine and tyrosine derivatives into proteins

Nour Eddine Fahmi, Larisa Dedkova, Bixun Wang, Serguei Golovine, Sidney M. Hecht

Research output: Contribution to journalArticle

28 Scopus citations

Abstract

Glycosylation of proteins can have a dramatic effect on their physical, chemical, and biological properties. Analogues of dihydrofolate reductase and firefly luciferase containing glycosylated amino acids at single, predetermined sites have been elaborated. Misacylated suppressor tRNAs activated with glycosylated serine and tyrosine derivatives were used for suppression of the nonsense codons in a cell-free protein biosynthesizing system, thereby permitting the preparation of the desired glycosylated proteins. In this fashion, it was possible to obtain proteins containing both mono- and diglycosylated amino acids, including glycosylated serine and tyrosine moieties. For the modified firefly luciferases, the effect of these substitutions on the wavelength of the light emitted by firefly luciferase was investigated. The maximum wavelength for mutants containing peracetylated glycosylated serine derivatives at position 284 showed a red shift in the emission spectra. For mutants containing glycosylated tyrosines, the red shift was observed only when the carbohydrate moiety was fully deacetylated.

Original languageEnglish (US)
Pages (from-to)3586-3597
Number of pages12
JournalJournal of the American Chemical Society
Volume129
Issue number12
DOIs
StatePublished - Mar 28 2007

ASJC Scopus subject areas

  • Catalysis
  • Chemistry(all)
  • Biochemistry
  • Colloid and Surface Chemistry

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