Single mimivirus particles intercepted and imaged with an X-ray laser

M. Marvin Seibert, Tomas Ekeberg, Filipe R N C Maia, Martin Svenda, Jakob Andreasson, Olof Jönsson, Duško Odić, Bianca Iwan, Andrea Rocker, Daniel Westphal, Max Hantke, Daniel P. Deponte, Anton Barty, Joachim Schulz, Lars Gumprecht, Nicola Coppola, Andrew Aquila, Mengning Liang, Thomas A. White, Andrew MartinCarl Caleman, Stephan Stern, Chantal Abergel, Virginie Seltzer, Jean Michel Claverie, Christoph Bostedt, John D. Bozek, Sébastien Boutet, A. Alan Miahnahri, Marc Messerschmidt, Jacek Krzywinski, Garth Williams, Keith O. Hodgson, Michael J. Bogan, Christina Y. Hampton, Raymond G. Sierra, Dmitri Starodub, Inger Andersson, Sǎa Bajt, Miriam Barthelmess, John Spence, Petra Fromme, Uwe Weierstall, Richard Kirian, Mark Hunter, R. Bruce Doak, Stefano Marchesini, Stefan P. Hau-Riege, Matthias Frank, Robert L. Shoeman, Lukas Lomb, Sascha W. Epp, Robert Hartmann, Daniel Rolles, Artem Rudenko, Carlo Schmidt, Lutz Foucar, Nils Kimmel, Peter Holl, Benedikt Rudek, Benjamin Erk, André Hömke, Christian Reich, Daniel Pietschner, Georg Weidenspointner, Lothar Strüder, Günter Hauser, Hubert Gorke, Joachim Ullrich, Ilme Schlichting, Sven Herrmann, Gerhard Schaller, Florian Schopper, Heike Soltau, Kai Uwe Kühnel, Robert Andritschke, Claus Dieter Schröter, Faton Krasniqi, Mario Bott, Sebastian Schorb, Daniela Rupp, Marcus Adolph, Tais Gorkhover, Helmut Hirsemann, Guillaume Potdevin, Heinz Graafsma, Björn Nilsson, Henry N. Chapman, Janos Hajdu

Research output: Contribution to journalArticlepeer-review

675 Scopus citations

Abstract

X-ray lasers offer new capabilities in understanding the structure of biological systems, complex materials and matter under extreme conditions. Very short and extremely bright, coherent X-ray pulses can be used to outrun key damage processes and obtain a single diffraction pattern from a large macromolecule, a virus or a cell before the sample explodes and turns into plasma. The continuous diffraction pattern of non-crystalline objects permits oversampling and direct phase retrieval. Here we show that high-quality diffraction data can be obtained with a single X-ray pulse from a non-crystalline biological sample, a single mimivirus particle, which was injected into the pulsed beam of a hard-X-ray free-electron laser, the Linac Coherent Light Source. Calculations indicate that the energy deposited into the virus by the pulse heated the particle to over 100,000K after the pulse had left the sample. The reconstructed exit wavefront (image) yielded 32-nm full-period resolution in a single exposure and showed no measurable damage. The reconstruction indicates inhomogeneous arrangement of dense material inside the virion. We expect that significantly higher resolutions will be achieved in such experiments with shorter and brighter photon pulses focused to a smaller area. The resolution in such experiments can be further extended for samples available in multiple identical copies.

Original languageEnglish (US)
Pages (from-to)78-82
Number of pages5
JournalNature
Volume470
Issue number7332
DOIs
StatePublished - Feb 3 2011

ASJC Scopus subject areas

  • General

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