Single mimivirus particles intercepted and imaged with an X-ray laser

M. Marvin Seibert, Tomas Ekeberg, Filipe R N C Maia, Martin Svenda, Jakob Andreasson, Olof Jönsson, Duško Odić, Bianca Iwan, Andrea Rocker, Daniel Westphal, Max Hantke, Daniel P. Deponte, Anton Barty, Joachim Schulz, Lars Gumprecht, Nicola Coppola, Andrew Aquila, Mengning Liang, Thomas A. White, Andrew MartinCarl Caleman, Stephan Stern, Chantal Abergel, Virginie Seltzer, Jean Michel Claverie, Christoph Bostedt, John D. Bozek, Sébastien Boutet, A. Alan Miahnahri, Marc Messerschmidt, Jacek Krzywinski, Garth Williams, Keith O. Hodgson, Michael J. Bogan, Christina Y. Hampton, Raymond G. Sierra, Dmitri Starodub, Inger Andersson, Sǎa Bajt, Miriam Barthelmess, John Spence, Petra Fromme, Uwe Weierstall, Richard Kirian, Mark Hunter, R. Bruce Doak, Stefano Marchesini, Stefan P. Hau-Riege, Matthias Frank, Robert L. Shoeman, Lukas Lomb, Sascha W. Epp, Robert Hartmann, Daniel Rolles, Artem Rudenko, Carlo Schmidt, Lutz Foucar, Nils Kimmel, Peter Holl, Benedikt Rudek, Benjamin Erk, André Hömke, Christian Reich, Daniel Pietschner, Georg Weidenspointner, Lothar Strüder, Günter Hauser, Hubert Gorke, Joachim Ullrich, Ilme Schlichting, Sven Herrmann, Gerhard Schaller, Florian Schopper, Heike Soltau, Kai Uwe Kühnel, Robert Andritschke, Claus Dieter Schröter, Faton Krasniqi, Mario Bott, Sebastian Schorb, Daniela Rupp, Marcus Adolph, Tais Gorkhover, Helmut Hirsemann, Guillaume Potdevin, Heinz Graafsma, Björn Nilsson, Henry N. Chapman, Janos Hajdu

Research output: Contribution to journalArticle

599 Citations (Scopus)

Abstract

X-ray lasers offer new capabilities in understanding the structure of biological systems, complex materials and matter under extreme conditions. Very short and extremely bright, coherent X-ray pulses can be used to outrun key damage processes and obtain a single diffraction pattern from a large macromolecule, a virus or a cell before the sample explodes and turns into plasma. The continuous diffraction pattern of non-crystalline objects permits oversampling and direct phase retrieval. Here we show that high-quality diffraction data can be obtained with a single X-ray pulse from a non-crystalline biological sample, a single mimivirus particle, which was injected into the pulsed beam of a hard-X-ray free-electron laser, the Linac Coherent Light Source. Calculations indicate that the energy deposited into the virus by the pulse heated the particle to over 100,000K after the pulse had left the sample. The reconstructed exit wavefront (image) yielded 32-nm full-period resolution in a single exposure and showed no measurable damage. The reconstruction indicates inhomogeneous arrangement of dense material inside the virion. We expect that significantly higher resolutions will be achieved in such experiments with shorter and brighter photon pulses focused to a smaller area. The resolution in such experiments can be further extended for samples available in multiple identical copies.

Original languageEnglish (US)
Pages (from-to)78-82
Number of pages5
JournalNature
Volume470
Issue number7332
DOIs
StatePublished - Feb 3 2011

Fingerprint

Mimiviridae
Lasers
X-Rays
Viruses
Photons
Virion
Electrons
Light

ASJC Scopus subject areas

  • General

Cite this

Seibert, M. M., Ekeberg, T., Maia, F. R. N. C., Svenda, M., Andreasson, J., Jönsson, O., ... Hajdu, J. (2011). Single mimivirus particles intercepted and imaged with an X-ray laser. Nature, 470(7332), 78-82. https://doi.org/10.1038/nature09748

Single mimivirus particles intercepted and imaged with an X-ray laser. / Seibert, M. Marvin; Ekeberg, Tomas; Maia, Filipe R N C; Svenda, Martin; Andreasson, Jakob; Jönsson, Olof; Odić, Duško; Iwan, Bianca; Rocker, Andrea; Westphal, Daniel; Hantke, Max; Deponte, Daniel P.; Barty, Anton; Schulz, Joachim; Gumprecht, Lars; Coppola, Nicola; Aquila, Andrew; Liang, Mengning; White, Thomas A.; Martin, Andrew; Caleman, Carl; Stern, Stephan; Abergel, Chantal; Seltzer, Virginie; Claverie, Jean Michel; Bostedt, Christoph; Bozek, John D.; Boutet, Sébastien; Miahnahri, A. Alan; Messerschmidt, Marc; Krzywinski, Jacek; Williams, Garth; Hodgson, Keith O.; Bogan, Michael J.; Hampton, Christina Y.; Sierra, Raymond G.; Starodub, Dmitri; Andersson, Inger; Bajt, Sǎa; Barthelmess, Miriam; Spence, John; Fromme, Petra; Weierstall, Uwe; Kirian, Richard; Hunter, Mark; Doak, R. Bruce; Marchesini, Stefano; Hau-Riege, Stefan P.; Frank, Matthias; Shoeman, Robert L.; Lomb, Lukas; Epp, Sascha W.; Hartmann, Robert; Rolles, Daniel; Rudenko, Artem; Schmidt, Carlo; Foucar, Lutz; Kimmel, Nils; Holl, Peter; Rudek, Benedikt; Erk, Benjamin; Hömke, André; Reich, Christian; Pietschner, Daniel; Weidenspointner, Georg; Strüder, Lothar; Hauser, Günter; Gorke, Hubert; Ullrich, Joachim; Schlichting, Ilme; Herrmann, Sven; Schaller, Gerhard; Schopper, Florian; Soltau, Heike; Kühnel, Kai Uwe; Andritschke, Robert; Schröter, Claus Dieter; Krasniqi, Faton; Bott, Mario; Schorb, Sebastian; Rupp, Daniela; Adolph, Marcus; Gorkhover, Tais; Hirsemann, Helmut; Potdevin, Guillaume; Graafsma, Heinz; Nilsson, Björn; Chapman, Henry N.; Hajdu, Janos.

In: Nature, Vol. 470, No. 7332, 03.02.2011, p. 78-82.

Research output: Contribution to journalArticle

Seibert, MM, Ekeberg, T, Maia, FRNC, Svenda, M, Andreasson, J, Jönsson, O, Odić, D, Iwan, B, Rocker, A, Westphal, D, Hantke, M, Deponte, DP, Barty, A, Schulz, J, Gumprecht, L, Coppola, N, Aquila, A, Liang, M, White, TA, Martin, A, Caleman, C, Stern, S, Abergel, C, Seltzer, V, Claverie, JM, Bostedt, C, Bozek, JD, Boutet, S, Miahnahri, AA, Messerschmidt, M, Krzywinski, J, Williams, G, Hodgson, KO, Bogan, MJ, Hampton, CY, Sierra, RG, Starodub, D, Andersson, I, Bajt, S, Barthelmess, M, Spence, J, Fromme, P, Weierstall, U, Kirian, R, Hunter, M, Doak, RB, Marchesini, S, Hau-Riege, SP, Frank, M, Shoeman, RL, Lomb, L, Epp, SW, Hartmann, R, Rolles, D, Rudenko, A, Schmidt, C, Foucar, L, Kimmel, N, Holl, P, Rudek, B, Erk, B, Hömke, A, Reich, C, Pietschner, D, Weidenspointner, G, Strüder, L, Hauser, G, Gorke, H, Ullrich, J, Schlichting, I, Herrmann, S, Schaller, G, Schopper, F, Soltau, H, Kühnel, KU, Andritschke, R, Schröter, CD, Krasniqi, F, Bott, M, Schorb, S, Rupp, D, Adolph, M, Gorkhover, T, Hirsemann, H, Potdevin, G, Graafsma, H, Nilsson, B, Chapman, HN & Hajdu, J 2011, 'Single mimivirus particles intercepted and imaged with an X-ray laser', Nature, vol. 470, no. 7332, pp. 78-82. https://doi.org/10.1038/nature09748
Seibert MM, Ekeberg T, Maia FRNC, Svenda M, Andreasson J, Jönsson O et al. Single mimivirus particles intercepted and imaged with an X-ray laser. Nature. 2011 Feb 3;470(7332):78-82. https://doi.org/10.1038/nature09748
Seibert, M. Marvin ; Ekeberg, Tomas ; Maia, Filipe R N C ; Svenda, Martin ; Andreasson, Jakob ; Jönsson, Olof ; Odić, Duško ; Iwan, Bianca ; Rocker, Andrea ; Westphal, Daniel ; Hantke, Max ; Deponte, Daniel P. ; Barty, Anton ; Schulz, Joachim ; Gumprecht, Lars ; Coppola, Nicola ; Aquila, Andrew ; Liang, Mengning ; White, Thomas A. ; Martin, Andrew ; Caleman, Carl ; Stern, Stephan ; Abergel, Chantal ; Seltzer, Virginie ; Claverie, Jean Michel ; Bostedt, Christoph ; Bozek, John D. ; Boutet, Sébastien ; Miahnahri, A. Alan ; Messerschmidt, Marc ; Krzywinski, Jacek ; Williams, Garth ; Hodgson, Keith O. ; Bogan, Michael J. ; Hampton, Christina Y. ; Sierra, Raymond G. ; Starodub, Dmitri ; Andersson, Inger ; Bajt, Sǎa ; Barthelmess, Miriam ; Spence, John ; Fromme, Petra ; Weierstall, Uwe ; Kirian, Richard ; Hunter, Mark ; Doak, R. Bruce ; Marchesini, Stefano ; Hau-Riege, Stefan P. ; Frank, Matthias ; Shoeman, Robert L. ; Lomb, Lukas ; Epp, Sascha W. ; Hartmann, Robert ; Rolles, Daniel ; Rudenko, Artem ; Schmidt, Carlo ; Foucar, Lutz ; Kimmel, Nils ; Holl, Peter ; Rudek, Benedikt ; Erk, Benjamin ; Hömke, André ; Reich, Christian ; Pietschner, Daniel ; Weidenspointner, Georg ; Strüder, Lothar ; Hauser, Günter ; Gorke, Hubert ; Ullrich, Joachim ; Schlichting, Ilme ; Herrmann, Sven ; Schaller, Gerhard ; Schopper, Florian ; Soltau, Heike ; Kühnel, Kai Uwe ; Andritschke, Robert ; Schröter, Claus Dieter ; Krasniqi, Faton ; Bott, Mario ; Schorb, Sebastian ; Rupp, Daniela ; Adolph, Marcus ; Gorkhover, Tais ; Hirsemann, Helmut ; Potdevin, Guillaume ; Graafsma, Heinz ; Nilsson, Björn ; Chapman, Henry N. ; Hajdu, Janos. / Single mimivirus particles intercepted and imaged with an X-ray laser. In: Nature. 2011 ; Vol. 470, No. 7332. pp. 78-82.
@article{86f55b04c08a4afe910203a013971216,
title = "Single mimivirus particles intercepted and imaged with an X-ray laser",
abstract = "X-ray lasers offer new capabilities in understanding the structure of biological systems, complex materials and matter under extreme conditions. Very short and extremely bright, coherent X-ray pulses can be used to outrun key damage processes and obtain a single diffraction pattern from a large macromolecule, a virus or a cell before the sample explodes and turns into plasma. The continuous diffraction pattern of non-crystalline objects permits oversampling and direct phase retrieval. Here we show that high-quality diffraction data can be obtained with a single X-ray pulse from a non-crystalline biological sample, a single mimivirus particle, which was injected into the pulsed beam of a hard-X-ray free-electron laser, the Linac Coherent Light Source. Calculations indicate that the energy deposited into the virus by the pulse heated the particle to over 100,000K after the pulse had left the sample. The reconstructed exit wavefront (image) yielded 32-nm full-period resolution in a single exposure and showed no measurable damage. The reconstruction indicates inhomogeneous arrangement of dense material inside the virion. We expect that significantly higher resolutions will be achieved in such experiments with shorter and brighter photon pulses focused to a smaller area. The resolution in such experiments can be further extended for samples available in multiple identical copies.",
author = "Seibert, {M. Marvin} and Tomas Ekeberg and Maia, {Filipe R N C} and Martin Svenda and Jakob Andreasson and Olof J{\"o}nsson and Duško Odić and Bianca Iwan and Andrea Rocker and Daniel Westphal and Max Hantke and Deponte, {Daniel P.} and Anton Barty and Joachim Schulz and Lars Gumprecht and Nicola Coppola and Andrew Aquila and Mengning Liang and White, {Thomas A.} and Andrew Martin and Carl Caleman and Stephan Stern and Chantal Abergel and Virginie Seltzer and Claverie, {Jean Michel} and Christoph Bostedt and Bozek, {John D.} and S{\'e}bastien Boutet and Miahnahri, {A. Alan} and Marc Messerschmidt and Jacek Krzywinski and Garth Williams and Hodgson, {Keith O.} and Bogan, {Michael J.} and Hampton, {Christina Y.} and Sierra, {Raymond G.} and Dmitri Starodub and Inger Andersson and Sǎa Bajt and Miriam Barthelmess and John Spence and Petra Fromme and Uwe Weierstall and Richard Kirian and Mark Hunter and Doak, {R. Bruce} and Stefano Marchesini and Hau-Riege, {Stefan P.} and Matthias Frank and Shoeman, {Robert L.} and Lukas Lomb and Epp, {Sascha W.} and Robert Hartmann and Daniel Rolles and Artem Rudenko and Carlo Schmidt and Lutz Foucar and Nils Kimmel and Peter Holl and Benedikt Rudek and Benjamin Erk and Andr{\'e} H{\"o}mke and Christian Reich and Daniel Pietschner and Georg Weidenspointner and Lothar Str{\"u}der and G{\"u}nter Hauser and Hubert Gorke and Joachim Ullrich and Ilme Schlichting and Sven Herrmann and Gerhard Schaller and Florian Schopper and Heike Soltau and K{\"u}hnel, {Kai Uwe} and Robert Andritschke and Schr{\"o}ter, {Claus Dieter} and Faton Krasniqi and Mario Bott and Sebastian Schorb and Daniela Rupp and Marcus Adolph and Tais Gorkhover and Helmut Hirsemann and Guillaume Potdevin and Heinz Graafsma and Bj{\"o}rn Nilsson and Chapman, {Henry N.} and Janos Hajdu",
year = "2011",
month = "2",
day = "3",
doi = "10.1038/nature09748",
language = "English (US)",
volume = "470",
pages = "78--82",
journal = "Nature",
issn = "0028-0836",
publisher = "Nature Publishing Group",
number = "7332",

}

TY - JOUR

T1 - Single mimivirus particles intercepted and imaged with an X-ray laser

AU - Seibert, M. Marvin

AU - Ekeberg, Tomas

AU - Maia, Filipe R N C

AU - Svenda, Martin

AU - Andreasson, Jakob

AU - Jönsson, Olof

AU - Odić, Duško

AU - Iwan, Bianca

AU - Rocker, Andrea

AU - Westphal, Daniel

AU - Hantke, Max

AU - Deponte, Daniel P.

AU - Barty, Anton

AU - Schulz, Joachim

AU - Gumprecht, Lars

AU - Coppola, Nicola

AU - Aquila, Andrew

AU - Liang, Mengning

AU - White, Thomas A.

AU - Martin, Andrew

AU - Caleman, Carl

AU - Stern, Stephan

AU - Abergel, Chantal

AU - Seltzer, Virginie

AU - Claverie, Jean Michel

AU - Bostedt, Christoph

AU - Bozek, John D.

AU - Boutet, Sébastien

AU - Miahnahri, A. Alan

AU - Messerschmidt, Marc

AU - Krzywinski, Jacek

AU - Williams, Garth

AU - Hodgson, Keith O.

AU - Bogan, Michael J.

AU - Hampton, Christina Y.

AU - Sierra, Raymond G.

AU - Starodub, Dmitri

AU - Andersson, Inger

AU - Bajt, Sǎa

AU - Barthelmess, Miriam

AU - Spence, John

AU - Fromme, Petra

AU - Weierstall, Uwe

AU - Kirian, Richard

AU - Hunter, Mark

AU - Doak, R. Bruce

AU - Marchesini, Stefano

AU - Hau-Riege, Stefan P.

AU - Frank, Matthias

AU - Shoeman, Robert L.

AU - Lomb, Lukas

AU - Epp, Sascha W.

AU - Hartmann, Robert

AU - Rolles, Daniel

AU - Rudenko, Artem

AU - Schmidt, Carlo

AU - Foucar, Lutz

AU - Kimmel, Nils

AU - Holl, Peter

AU - Rudek, Benedikt

AU - Erk, Benjamin

AU - Hömke, André

AU - Reich, Christian

AU - Pietschner, Daniel

AU - Weidenspointner, Georg

AU - Strüder, Lothar

AU - Hauser, Günter

AU - Gorke, Hubert

AU - Ullrich, Joachim

AU - Schlichting, Ilme

AU - Herrmann, Sven

AU - Schaller, Gerhard

AU - Schopper, Florian

AU - Soltau, Heike

AU - Kühnel, Kai Uwe

AU - Andritschke, Robert

AU - Schröter, Claus Dieter

AU - Krasniqi, Faton

AU - Bott, Mario

AU - Schorb, Sebastian

AU - Rupp, Daniela

AU - Adolph, Marcus

AU - Gorkhover, Tais

AU - Hirsemann, Helmut

AU - Potdevin, Guillaume

AU - Graafsma, Heinz

AU - Nilsson, Björn

AU - Chapman, Henry N.

AU - Hajdu, Janos

PY - 2011/2/3

Y1 - 2011/2/3

N2 - X-ray lasers offer new capabilities in understanding the structure of biological systems, complex materials and matter under extreme conditions. Very short and extremely bright, coherent X-ray pulses can be used to outrun key damage processes and obtain a single diffraction pattern from a large macromolecule, a virus or a cell before the sample explodes and turns into plasma. The continuous diffraction pattern of non-crystalline objects permits oversampling and direct phase retrieval. Here we show that high-quality diffraction data can be obtained with a single X-ray pulse from a non-crystalline biological sample, a single mimivirus particle, which was injected into the pulsed beam of a hard-X-ray free-electron laser, the Linac Coherent Light Source. Calculations indicate that the energy deposited into the virus by the pulse heated the particle to over 100,000K after the pulse had left the sample. The reconstructed exit wavefront (image) yielded 32-nm full-period resolution in a single exposure and showed no measurable damage. The reconstruction indicates inhomogeneous arrangement of dense material inside the virion. We expect that significantly higher resolutions will be achieved in such experiments with shorter and brighter photon pulses focused to a smaller area. The resolution in such experiments can be further extended for samples available in multiple identical copies.

AB - X-ray lasers offer new capabilities in understanding the structure of biological systems, complex materials and matter under extreme conditions. Very short and extremely bright, coherent X-ray pulses can be used to outrun key damage processes and obtain a single diffraction pattern from a large macromolecule, a virus or a cell before the sample explodes and turns into plasma. The continuous diffraction pattern of non-crystalline objects permits oversampling and direct phase retrieval. Here we show that high-quality diffraction data can be obtained with a single X-ray pulse from a non-crystalline biological sample, a single mimivirus particle, which was injected into the pulsed beam of a hard-X-ray free-electron laser, the Linac Coherent Light Source. Calculations indicate that the energy deposited into the virus by the pulse heated the particle to over 100,000K after the pulse had left the sample. The reconstructed exit wavefront (image) yielded 32-nm full-period resolution in a single exposure and showed no measurable damage. The reconstruction indicates inhomogeneous arrangement of dense material inside the virion. We expect that significantly higher resolutions will be achieved in such experiments with shorter and brighter photon pulses focused to a smaller area. The resolution in such experiments can be further extended for samples available in multiple identical copies.

UR - http://www.scopus.com/inward/record.url?scp=79551667263&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=79551667263&partnerID=8YFLogxK

U2 - 10.1038/nature09748

DO - 10.1038/nature09748

M3 - Article

C2 - 21293374

AN - SCOPUS:79551667263

VL - 470

SP - 78

EP - 82

JO - Nature

JF - Nature

SN - 0028-0836

IS - 7332

ER -