Simultaneous topography and RECognition mapping with PicoTREC™: A powerful new technology that can be used to map nanometer-scale molecular binding sites on a variety of surfaces

W. T. Johnson, G. Kada, C. Stroh, H. Gruber, H. Wang, F. Kienberger, A. Ebner, Stuart Lindsay, P. Hinterdorfer

Research output: Chapter in Book/Report/Conference proceedingConference contribution

6 Citations (Scopus)

Abstract

PicoTREC™ is a powerful new chemical/biological detection technology that combines nanoscale Topographic imaging the AFM with single molecule RECognition mapping. The technique has been used to map interactions between, for example charged species, antibodies/antigens, and ligands/receptors in order to analyze the chemical composition of a variety of samples. PicoTREC combines in-situ nanometer scale, resolution imaging with the selectivity and sensitivity of single molecule, piconewton sensitivity to resolve and detect recognition events. It is label-less, so it is not dependent on fluorescence, radioactivity, or enzyme-linked detection schemes. When a ligand for a particular receptor is attached to an AFM tip, the AFM tip becomes a chemically selective sensor so that the forces required to break chemical bonds with the specific binding epitopes on target molecules can be detected and resolved. Consequently, maps of binding sites across a variety surfaces can quickly and easily be obtained.

Original languageEnglish (US)
Title of host publication2005 NSTI Nanotechnology Conference and Trade Show - NSTI Nanotech 2005 Technical Proceedings
EditorsM. Laudon, B. Romanowicz
Pages679-682
Number of pages4
StatePublished - 2005
Event2005 NSTI Nanotechnology Conference and Trade Show - NSTI Nanotech 2005 - Anaheim, CA, United States
Duration: May 8 2005May 12 2005

Other

Other2005 NSTI Nanotechnology Conference and Trade Show - NSTI Nanotech 2005
CountryUnited States
CityAnaheim, CA
Period5/8/055/12/05

Fingerprint

Binding sites
Topography
Molecules
Ligands
Epitopes
Imaging techniques
Chemical bonds
Radioactivity
Antigens
Antibodies
Labels
Enzymes
Fluorescence
Sensors
Chemical analysis

Keywords

  • AFM
  • Antibody-antigen
  • Biosensor
  • Ligand-receptor
  • Nanobiotechnology

ASJC Scopus subject areas

  • Engineering(all)

Cite this

Johnson, W. T., Kada, G., Stroh, C., Gruber, H., Wang, H., Kienberger, F., ... Hinterdorfer, P. (2005). Simultaneous topography and RECognition mapping with PicoTREC™: A powerful new technology that can be used to map nanometer-scale molecular binding sites on a variety of surfaces. In M. Laudon, & B. Romanowicz (Eds.), 2005 NSTI Nanotechnology Conference and Trade Show - NSTI Nanotech 2005 Technical Proceedings (pp. 679-682)

Simultaneous topography and RECognition mapping with PicoTREC™ : A powerful new technology that can be used to map nanometer-scale molecular binding sites on a variety of surfaces. / Johnson, W. T.; Kada, G.; Stroh, C.; Gruber, H.; Wang, H.; Kienberger, F.; Ebner, A.; Lindsay, Stuart; Hinterdorfer, P.

2005 NSTI Nanotechnology Conference and Trade Show - NSTI Nanotech 2005 Technical Proceedings. ed. / M. Laudon; B. Romanowicz. 2005. p. 679-682.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Johnson, WT, Kada, G, Stroh, C, Gruber, H, Wang, H, Kienberger, F, Ebner, A, Lindsay, S & Hinterdorfer, P 2005, Simultaneous topography and RECognition mapping with PicoTREC™: A powerful new technology that can be used to map nanometer-scale molecular binding sites on a variety of surfaces. in M Laudon & B Romanowicz (eds), 2005 NSTI Nanotechnology Conference and Trade Show - NSTI Nanotech 2005 Technical Proceedings. pp. 679-682, 2005 NSTI Nanotechnology Conference and Trade Show - NSTI Nanotech 2005, Anaheim, CA, United States, 5/8/05.
Johnson WT, Kada G, Stroh C, Gruber H, Wang H, Kienberger F et al. Simultaneous topography and RECognition mapping with PicoTREC™: A powerful new technology that can be used to map nanometer-scale molecular binding sites on a variety of surfaces. In Laudon M, Romanowicz B, editors, 2005 NSTI Nanotechnology Conference and Trade Show - NSTI Nanotech 2005 Technical Proceedings. 2005. p. 679-682
Johnson, W. T. ; Kada, G. ; Stroh, C. ; Gruber, H. ; Wang, H. ; Kienberger, F. ; Ebner, A. ; Lindsay, Stuart ; Hinterdorfer, P. / Simultaneous topography and RECognition mapping with PicoTREC™ : A powerful new technology that can be used to map nanometer-scale molecular binding sites on a variety of surfaces. 2005 NSTI Nanotechnology Conference and Trade Show - NSTI Nanotech 2005 Technical Proceedings. editor / M. Laudon ; B. Romanowicz. 2005. pp. 679-682
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abstract = "PicoTREC™ is a powerful new chemical/biological detection technology that combines nanoscale Topographic imaging the AFM with single molecule RECognition mapping. The technique has been used to map interactions between, for example charged species, antibodies/antigens, and ligands/receptors in order to analyze the chemical composition of a variety of samples. PicoTREC combines in-situ nanometer scale, resolution imaging with the selectivity and sensitivity of single molecule, piconewton sensitivity to resolve and detect recognition events. It is label-less, so it is not dependent on fluorescence, radioactivity, or enzyme-linked detection schemes. When a ligand for a particular receptor is attached to an AFM tip, the AFM tip becomes a chemically selective sensor so that the forces required to break chemical bonds with the specific binding epitopes on target molecules can be detected and resolved. Consequently, maps of binding sites across a variety surfaces can quickly and easily be obtained.",
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