Signatures of natural selection on mutations of residues with multiple posttranslational modifications

Vanessa E. Gray, Li Liu, Ronika Nirankari, Peter V. Hornbeck, Sudhir Kumar

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Posttranslational modifications (PTMs) regulate molecular structures and functions of proteins by covalently binding to amino acids. Hundreds of thousands of PTMs have been reported for the human proteome, with multiple PTMs known to affect tens of thousands of lysine (K) residues. Our molecular evolutionary analyses show that K residues with multiple PTMs exhibit greater conservation than those with a single PTM, but the difference is rather small. In contrast, short-term evolutionary trends revealed in an analysis of human population variation exhibited a much larger difference. Lysine residues with three PTMs show 1.8-fold enrichment of Mendelian disease-associated variants when compared with K residues with two PTMs, with the latter showing 1.7-fold enrichment of these variants when compared with the K residues with one PTM. Rare polymorphisms in humans show a similar trend, which suggests much greater negative selection against mutations of K residues with multiple PTMs within population. Conversely, common polymorphisms are overabundant at unmodified K residues and at K residues with fewer PTMs. The observed difference between inter- and intraspecies patterns of purifying selection on residues with PTMs suggests extensive species-specific drifting of PTM positions. These results suggest that the functionality of a protein is likely conserved, without necessarily conserving the PTM positions over evolutionary time.

Original languageEnglish (US)
Pages (from-to)1641-1645
Number of pages5
JournalMolecular Biology and Evolution
Volume31
Issue number7
DOIs
StatePublished - 2014

Fingerprint

Genetic Selection
post-translational modification
Post Translational Protein Processing
natural selection
mutation
polymorphism
fold
Mutation
protein
amino acid
trend
Lysine
lysine
genetic polymorphism
Proteome
proteome
Molecular Structure
Protein Binding
chemical structure
human population

Keywords

  • evolution
  • posttranslational modification
  • proteomics

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology
  • Ecology, Evolution, Behavior and Systematics
  • Medicine(all)

Cite this

Signatures of natural selection on mutations of residues with multiple posttranslational modifications. / Gray, Vanessa E.; Liu, Li; Nirankari, Ronika; Hornbeck, Peter V.; Kumar, Sudhir.

In: Molecular Biology and Evolution, Vol. 31, No. 7, 2014, p. 1641-1645.

Research output: Contribution to journalArticle

Gray, Vanessa E. ; Liu, Li ; Nirankari, Ronika ; Hornbeck, Peter V. ; Kumar, Sudhir. / Signatures of natural selection on mutations of residues with multiple posttranslational modifications. In: Molecular Biology and Evolution. 2014 ; Vol. 31, No. 7. pp. 1641-1645.
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