Signaling Complexes of the FERM Domain-containing Protein GRSP1 Bound to ARF Exchange Factor GRP1

Jes K. Klarlund, John Holik, Anil Chawla, Jin Park, Joanne Buxton, Michael P. Czech

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

GRP1 is a member of a family of proteins that contain a coiled-coil region, a Sec7 homology domain with guanosine nucleotide exchange activity for the ARF GTP-binding proteins, and a pleckstrin homology domain at the C terminus. The pleckstrin homology domain of GRP1 binds phosphatidylinositol (3,4,5) trisphosphate and mediates the translocation of GRP1 to the plasma membrane upon agonist stimulation of PI 3-kinase activity. Using a 32P-labeled GRP1 probe to screen a mouse brain cDNA expression library, we isolated a cDNA clone encoding a GRP1-binding partner (GRSP1) that exists as two different splice variants in brain and lung. The GRSP1 protein contains a FERM protein interaction domain as well as two coiled coil domains and may therefore function as a scaffolding protein. Mapping experiments revealed that the interaction of GRP1 and GRSP1 occurs through the coiled coil domains in the two proteins. Immunodepletion experiments indicate that virtually all of the endogenous GRSP1 protein exists as a complex with GRP1 in lung. When co-expressed in Chinese hamster ovary cells expressing the human insulin receptor, both proteins display a diffuse, cytoplasmic localization. Acute translocation and co-localization of GRSP1 and GRP1 to ruffles in the plasma membrane was evident after insulin stimulation. These results identify GRSP1 as a novel member of GRP1 signaling complexes that are acutely recruited to plasma membrane ruffles in response to insulin receptor signaling.

Original languageEnglish (US)
Pages (from-to)40065-40070
Number of pages6
JournalJournal of Biological Chemistry
Volume276
Issue number43
DOIs
StatePublished - Oct 26 2001
Externally publishedYes

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Cell membranes
Proteins
Cell Membrane
Brain
Complementary DNA
Protein Interaction Domains and Motifs
Lung
Guanosine
Insulin Receptor
Cricetulus
Phosphatidylinositol 3-Kinases
Gene Library
GTP-Binding Proteins
Protein Domains
Ovary
Nucleotides
Clone Cells
Experiments
Cells
Insulin

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Signaling Complexes of the FERM Domain-containing Protein GRSP1 Bound to ARF Exchange Factor GRP1. / Klarlund, Jes K.; Holik, John; Chawla, Anil; Park, Jin; Buxton, Joanne; Czech, Michael P.

In: Journal of Biological Chemistry, Vol. 276, No. 43, 26.10.2001, p. 40065-40070.

Research output: Contribution to journalArticle

Klarlund, Jes K. ; Holik, John ; Chawla, Anil ; Park, Jin ; Buxton, Joanne ; Czech, Michael P. / Signaling Complexes of the FERM Domain-containing Protein GRSP1 Bound to ARF Exchange Factor GRP1. In: Journal of Biological Chemistry. 2001 ; Vol. 276, No. 43. pp. 40065-40070.
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