Serial femtosecond X-ray diffraction of enveloped virus microcrystals

Robert M. Lawrence, Chelsie E. Conrad, Nadia Zatsepin, Thomas D. Grant, Haiguang Liu, Daniel James, Garrett Nelson, Ganesh Subramanian, Andrew Aquila, Mark S. Hunter, Mengning Liang, Sébastien Boutet, Jesse Coe, John Spence, Uwe Weierstall, Wei Liu, Petra Fromme, Vadim Cherezov, Brenda Hogue

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Serial femtosecond crystallography (SFX) using X-ray free-electron lasers has produced high-resolution, room temperature, time-resolved protein structures. We report preliminary SFX of Sindbis virus, an enveloped icosahedral RNA virus with ~700A ° diameter. Microcrystals delivered in viscous agarose medium diffracted to ~40A ° resolution. Small-angle diffuse X-ray scattering overlaid Bragg peaks and analysis suggests this results from molecular transforms of individual particles. Viral proteins undergo structural changes during entry and infection, which could, in principle, be studied with SFX. This is an important step toward determining room temperature structures from virus microcrystals that may enable time-resolved studies of enveloped viruses.

Original languageEnglish (US)
Article number041720
JournalStructural Dynamics
Volume2
Issue number4
DOIs
StatePublished - Jul 1 2015

Fingerprint

Microcrystals
microcrystals
viruses
Viruses
X ray diffraction
diffraction
x rays
X ray lasers
Viral Structural Proteins
proteins
Proteins
Time and motion study
Crystallography
Free electron lasers
room temperature
infectious diseases
X ray scattering
RNA
entry
free electron lasers

ASJC Scopus subject areas

  • Condensed Matter Physics
  • Instrumentation
  • Radiation
  • Spectroscopy

Cite this

Lawrence, R. M., Conrad, C. E., Zatsepin, N., Grant, T. D., Liu, H., James, D., ... Hogue, B. (2015). Serial femtosecond X-ray diffraction of enveloped virus microcrystals. Structural Dynamics, 2(4), [041720]. https://doi.org/10.1063/1.4929410

Serial femtosecond X-ray diffraction of enveloped virus microcrystals. / Lawrence, Robert M.; Conrad, Chelsie E.; Zatsepin, Nadia; Grant, Thomas D.; Liu, Haiguang; James, Daniel; Nelson, Garrett; Subramanian, Ganesh; Aquila, Andrew; Hunter, Mark S.; Liang, Mengning; Boutet, Sébastien; Coe, Jesse; Spence, John; Weierstall, Uwe; Liu, Wei; Fromme, Petra; Cherezov, Vadim; Hogue, Brenda.

In: Structural Dynamics, Vol. 2, No. 4, 041720, 01.07.2015.

Research output: Contribution to journalArticle

Lawrence, RM, Conrad, CE, Zatsepin, N, Grant, TD, Liu, H, James, D, Nelson, G, Subramanian, G, Aquila, A, Hunter, MS, Liang, M, Boutet, S, Coe, J, Spence, J, Weierstall, U, Liu, W, Fromme, P, Cherezov, V & Hogue, B 2015, 'Serial femtosecond X-ray diffraction of enveloped virus microcrystals', Structural Dynamics, vol. 2, no. 4, 041720. https://doi.org/10.1063/1.4929410
Lawrence RM, Conrad CE, Zatsepin N, Grant TD, Liu H, James D et al. Serial femtosecond X-ray diffraction of enveloped virus microcrystals. Structural Dynamics. 2015 Jul 1;2(4). 041720. https://doi.org/10.1063/1.4929410
Lawrence, Robert M. ; Conrad, Chelsie E. ; Zatsepin, Nadia ; Grant, Thomas D. ; Liu, Haiguang ; James, Daniel ; Nelson, Garrett ; Subramanian, Ganesh ; Aquila, Andrew ; Hunter, Mark S. ; Liang, Mengning ; Boutet, Sébastien ; Coe, Jesse ; Spence, John ; Weierstall, Uwe ; Liu, Wei ; Fromme, Petra ; Cherezov, Vadim ; Hogue, Brenda. / Serial femtosecond X-ray diffraction of enveloped virus microcrystals. In: Structural Dynamics. 2015 ; Vol. 2, No. 4.
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