Serial femtosecond X-ray diffraction of enveloped virus microcrystals

Robert M. Lawrence; Chelsie E. Conrad; Nadia Zatsepin; Thomas D. Grant; Haiguang Liu; Daniel James; Garrett Nelson; Ganesh Subramanian; Andrew Aquila; Mark S. Hunter; Mengning Liang; Sébastien Boutet; Jesse Coe; John Spence; Uwe Weierstall; Wei Liu; Petra Fromme; Vadim Cherezov; Brenda Hogue

doi:10.1063/1.4929410

Serial femtosecond X-ray diffraction of enveloped virus microcrystals

Robert M. Lawrence, Chelsie E. Conrad, Nadia Zatsepin, Thomas D. Grant, Haiguang Liu, Daniel James, Garrett Nelson, Ganesh Subramanian, Andrew Aquila, Mark S. Hunter, Mengning Liang, Sébastien Boutet, Jesse Coe, John Spence, Uwe Weierstall, Wei Liu, Petra Fromme, Vadim Cherezov, Brenda Hogue

Research output: Contribution to journal › Article › peer-review

11 Scopus citations

Abstract

Serial femtosecond crystallography (SFX) using X-ray free-electron lasers has produced high-resolution, room temperature, time-resolved protein structures. We report preliminary SFX of Sindbis virus, an enveloped icosahedral RNA virus with ~700A ° diameter. Microcrystals delivered in viscous agarose medium diffracted to ~40A ° resolution. Small-angle diffuse X-ray scattering overlaid Bragg peaks and analysis suggests this results from molecular transforms of individual particles. Viral proteins undergo structural changes during entry and infection, which could, in principle, be studied with SFX. This is an important step toward determining room temperature structures from virus microcrystals that may enable time-resolved studies of enveloped viruses.

Original language	English (US)
Article number	041720
Journal	Structural Dynamics
Volume	2
Issue number	4
DOIs	https://doi.org/10.1063/1.4929410
State	Published - Jul 1 2015

ASJC Scopus subject areas

Radiation
Instrumentation
Condensed Matter Physics
Spectroscopy

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Lawrence, R. M., Conrad, C. E., Zatsepin, N., Grant, T. D., Liu, H., James, D., Nelson, G., Subramanian, G., Aquila, A., Hunter, M. S., Liang, M., Boutet, S., Coe, J., Spence, J., Weierstall, U., Liu, W., Fromme, P., Cherezov, V., & Hogue, B. (2015). Serial femtosecond X-ray diffraction of enveloped virus microcrystals. Structural Dynamics, 2(4), Article 041720. https://doi.org/10.1063/1.4929410

Lawrence, RM, Conrad, CE, Zatsepin, N, Grant, TD, Liu, H, James, D, Nelson, G, Subramanian, G, Aquila, A, Hunter, MS, Liang, M, Boutet, S, Coe, J, Spence, J, Weierstall, U, Liu, W, Fromme, P, Cherezov, V & Hogue, B 2015, 'Serial femtosecond X-ray diffraction of enveloped virus microcrystals', Structural Dynamics, vol. 2, no. 4, 041720. https://doi.org/10.1063/1.4929410

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abstract = "Serial femtosecond crystallography (SFX) using X-ray free-electron lasers has produced high-resolution, room temperature, time-resolved protein structures. We report preliminary SFX of Sindbis virus, an enveloped icosahedral RNA virus with ~700A ° diameter. Microcrystals delivered in viscous agarose medium diffracted to ~40A ° resolution. Small-angle diffuse X-ray scattering overlaid Bragg peaks and analysis suggests this results from molecular transforms of individual particles. Viral proteins undergo structural changes during entry and infection, which could, in principle, be studied with SFX. This is an important step toward determining room temperature structures from virus microcrystals that may enable time-resolved studies of enveloped viruses.",

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AU - Liu, Haiguang

AU - James, Daniel

AU - Nelson, Garrett

AU - Subramanian, Ganesh

AU - Aquila, Andrew

AU - Hunter, Mark S.

AU - Liang, Mengning

AU - Boutet, Sébastien

AU - Coe, Jesse

AU - Spence, John

AU - Weierstall, Uwe

AU - Liu, Wei

AU - Fromme, Petra

AU - Cherezov, Vadim

AU - Hogue, Brenda

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AB - Serial femtosecond crystallography (SFX) using X-ray free-electron lasers has produced high-resolution, room temperature, time-resolved protein structures. We report preliminary SFX of Sindbis virus, an enveloped icosahedral RNA virus with ~700A ° diameter. Microcrystals delivered in viscous agarose medium diffracted to ~40A ° resolution. Small-angle diffuse X-ray scattering overlaid Bragg peaks and analysis suggests this results from molecular transforms of individual particles. Viral proteins undergo structural changes during entry and infection, which could, in principle, be studied with SFX. This is an important step toward determining room temperature structures from virus microcrystals that may enable time-resolved studies of enveloped viruses.

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Serial femtosecond X-ray diffraction of enveloped virus microcrystals

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