Serial femtosecond crystallography of membrane proteins

Lan Zhu, Uwe Weierstall, Vadim Cherezov, Wei Liu

Research output: Contribution to journalArticle

Abstract

Membrane proteins, including G protein-coupled receptors (GPCRs), constitute the most important drug targets. The increasing number of targets requires new structural information, which has proven tremendously challenging due to the difficulties in growing diffraction-quality crystals. Recent developments of serial femtosecond crystallography at X-ray free electron lasers combined with the use of membrane-mimetic gel-like matrix of lipidic cubic phase (LCP-SFX) for crystal growth and delivery hold significant promise to accelerate structural studies of membrane proteins. This chapter describes the development and current status of the LCP-SFX technology and elaborates its future role in structural biology of membrane proteins.

Original languageEnglish (US)
Pages (from-to)151-160
Number of pages10
JournalAdvances in Experimental Medicine and Biology
Volume922
DOIs
StatePublished - 2016

Fingerprint

Crystallography
Membrane Proteins
X ray lasers
Free electron lasers
X Ray Crystallography
G-Protein-Coupled Receptors
Crystallization
Lasers
Diffraction
Gels
Electrons
Technology
Membranes
Crystals
Pharmaceutical Preparations

Keywords

  • G proteincoupled receptors
  • LCP injector
  • LCP-SFX
  • Lipidic cubic phase
  • Membrane proteins
  • Serial femtosecond crystallography
  • X-ray free electron laser

ASJC Scopus subject areas

  • Medicine(all)
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Serial femtosecond crystallography of membrane proteins. / Zhu, Lan; Weierstall, Uwe; Cherezov, Vadim; Liu, Wei.

In: Advances in Experimental Medicine and Biology, Vol. 922, 2016, p. 151-160.

Research output: Contribution to journalArticle

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