Serial femtosecond crystallography of membrane proteins

Lan Zhu; Uwe Weierstall; Vadim Cherezov; Wei Liu

doi:10.1007/978-3-319-35072-1_11

Serial femtosecond crystallography of membrane proteins

Lan Zhu, Uwe Weierstall, Vadim Cherezov, Wei Liu

Research output: Contribution to journal › Article › peer-review

8 Scopus citations

Abstract

Membrane proteins, including G protein-coupled receptors (GPCRs), constitute the most important drug targets. The increasing number of targets requires new structural information, which has proven tremendously challenging due to the difficulties in growing diffraction-quality crystals. Recent developments of serial femtosecond crystallography at X-ray free electron lasers combined with the use of membrane-mimetic gel-like matrix of lipidic cubic phase (LCP-SFX) for crystal growth and delivery hold significant promise to accelerate structural studies of membrane proteins. This chapter describes the development and current status of the LCP-SFX technology and elaborates its future role in structural biology of membrane proteins.

Original language	English (US)
Pages (from-to)	151-160
Number of pages	10
Journal	Advances in experimental medicine and biology
Volume	922
DOIs	https://doi.org/10.1007/978-3-319-35072-1_11
State	Published - 2016

Keywords

G proteincoupled receptors
LCP injector
LCP-SFX
Lipidic cubic phase
Membrane proteins
Serial femtosecond crystallography
X-ray free electron laser

ASJC Scopus subject areas

General Biochemistry, Genetics and Molecular Biology

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10.1007/978-3-319-35072-1_11

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title = "Serial femtosecond crystallography of membrane proteins",

abstract = "Membrane proteins, including G protein-coupled receptors (GPCRs), constitute the most important drug targets. The increasing number of targets requires new structural information, which has proven tremendously challenging due to the difficulties in growing diffraction-quality crystals. Recent developments of serial femtosecond crystallography at X-ray free electron lasers combined with the use of membrane-mimetic gel-like matrix of lipidic cubic phase (LCP-SFX) for crystal growth and delivery hold significant promise to accelerate structural studies of membrane proteins. This chapter describes the development and current status of the LCP-SFX technology and elaborates its future role in structural biology of membrane proteins.",

keywords = "G proteincoupled receptors, LCP injector, LCP-SFX, Lipidic cubic phase, Membrane proteins, Serial femtosecond crystallography, X-ray free electron laser",

author = "Lan Zhu and Uwe Weierstall and Vadim Cherezov and Wei Liu",

note = "Funding Information: This work was supported in parts by the NIH grants R01 GM108635 and U54 GM094618 (V.C.), NSF BioXFEL Science and Technology center grant 1231306 (U.W. and W.L.). Further supports were provided by the Arizona State University (ASU) Biodesign Seed Grant Program and ASU-Mayo Seed Grant Program (L.Z. and W.L.). Parts of this research were carried out at the LCLS, a National User Facility operated by Stanford University on behalf of the U.S. Department of Energy, Office of Basic Energy Sciences, and at the GM/CA CAT of the Argonne Photon Source, Argonne National Laboratory. Publisher Copyright: {\textcopyright} Springer International Publishing Switzerland 2016.",

year = "2016",

doi = "10.1007/978-3-319-35072-1_11",

language = "English (US)",

volume = "922",

pages = "151--160",

journal = "Advances in experimental medicine and biology",

issn = "0065-2598",

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T1 - Serial femtosecond crystallography of membrane proteins

AU - Zhu, Lan

AU - Weierstall, Uwe

AU - Cherezov, Vadim

AU - Liu, Wei

N1 - Funding Information: This work was supported in parts by the NIH grants R01 GM108635 and U54 GM094618 (V.C.), NSF BioXFEL Science and Technology center grant 1231306 (U.W. and W.L.). Further supports were provided by the Arizona State University (ASU) Biodesign Seed Grant Program and ASU-Mayo Seed Grant Program (L.Z. and W.L.). Parts of this research were carried out at the LCLS, a National User Facility operated by Stanford University on behalf of the U.S. Department of Energy, Office of Basic Energy Sciences, and at the GM/CA CAT of the Argonne Photon Source, Argonne National Laboratory. Publisher Copyright: © Springer International Publishing Switzerland 2016.

PY - 2016

Y1 - 2016

N2 - Membrane proteins, including G protein-coupled receptors (GPCRs), constitute the most important drug targets. The increasing number of targets requires new structural information, which has proven tremendously challenging due to the difficulties in growing diffraction-quality crystals. Recent developments of serial femtosecond crystallography at X-ray free electron lasers combined with the use of membrane-mimetic gel-like matrix of lipidic cubic phase (LCP-SFX) for crystal growth and delivery hold significant promise to accelerate structural studies of membrane proteins. This chapter describes the development and current status of the LCP-SFX technology and elaborates its future role in structural biology of membrane proteins.

AB - Membrane proteins, including G protein-coupled receptors (GPCRs), constitute the most important drug targets. The increasing number of targets requires new structural information, which has proven tremendously challenging due to the difficulties in growing diffraction-quality crystals. Recent developments of serial femtosecond crystallography at X-ray free electron lasers combined with the use of membrane-mimetic gel-like matrix of lipidic cubic phase (LCP-SFX) for crystal growth and delivery hold significant promise to accelerate structural studies of membrane proteins. This chapter describes the development and current status of the LCP-SFX technology and elaborates its future role in structural biology of membrane proteins.

KW - G proteincoupled receptors

KW - LCP injector

KW - LCP-SFX

KW - Lipidic cubic phase

KW - Membrane proteins

KW - Serial femtosecond crystallography

KW - X-ray free electron laser

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SN - 0065-2598

VL - 922

SP - 151

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JO - Advances in experimental medicine and biology

JF - Advances in experimental medicine and biology

ER -

Serial femtosecond crystallography of membrane proteins

Abstract

Keywords

ASJC Scopus subject areas

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