Sequence-dependent photophysical properties of Cy3-labeled DNA

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We have investigated the photophysical properties of Cy3-labeled DNA. Results show that the fluorescence efficiency of the dye changes dramatically upon covalent attachment, and that factors such as DNA sequence, or whether the dye is next to a single- or double-stranded region, also play a crucial role. Such dramatic dependence on the microscopic environment of the probe is due to the existence of an activated photoisomerization process that competes with fluorescence emission to deactivate the excited state. Cy3-DNA interactions, which depend on DNA sequence and the flexibility of the biopolymer, restrict the ability of the dye to photoisomerize and therefore increase the lifetime of the excited state. In this manuscript, we review the results of our most recent studies, and discuss the consequences of the photophysical behavior of Cy3 in biophysical research.

Original languageEnglish (US)
Title of host publicationReporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications II
DOIs
StatePublished - May 10 2010
EventReporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications II - San Francisco, CA, United States
Duration: Jan 25 2010Jan 27 2010

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume7576
ISSN (Print)1605-7422

Other

OtherReporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications II
CountryUnited States
CitySan Francisco, CA
Period1/25/101/27/10

Keywords

  • Anisotropy
  • Cy5
  • Cyanines
  • Energy transfer
  • FRET
  • Fluorescence
  • Quenching
  • Single-molecule

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Radiology Nuclear Medicine and imaging

Fingerprint Dive into the research topics of 'Sequence-dependent photophysical properties of Cy3-labeled DNA'. Together they form a unique fingerprint.

  • Cite this

    Levitus, M. (2010). Sequence-dependent photophysical properties of Cy3-labeled DNA. In Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications II [75761D] (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 7576). https://doi.org/10.1117/12.841060