Abstract

This article reports a MEMS (Micro-Electro-Mechanical-Systems)-based column separator designed for potential integration with portable medical point-of-care testing (POCT) devices. The MEMS column uses size exclusion chromatography (SEC) to pre-separate raw samples by size, and is made of polydimethylsiloxane (PDMS) fabricated on a glass slide. The MEMS SEC column separates 300 ng/mL of beta-human chorionic gonadotropin (β-hCG), a cancer biomarker, from a fibrin-ogen-rich solution (20 μg/mL) in 10 min, showing 2.12 resolution and 0.036 mm plate height through fluorescent detection. Results are further verified by β-hCG and anti-β-hCG antibody conjugate using surface plasmon resonance (SPR). The collected β-hCG-rich eluent at 8 min shows 11 mDeg of angle shift. The fluorescent detection and SPR results demonstrate the complete discrimination of β-hCG from fibrinogen using the MEMS SEC column, and illustrate its viability for integrating a sample preparation stage in POCT devices to assist cancer screening and prognosis.

Original languageEnglish (US)
Pages (from-to)477-484
Number of pages8
JournalMicrofluidics and Nanofluidics
Volume8
Issue number4
DOIs
StatePublished - Apr 2010

Fingerprint

fibrinogen
Size exclusion chromatography
Chorionic Gonadotropin
chromatography
exclusion
Fibrinogen
Surface plasmon resonance
surface plasmon resonance
cancer
Testing
fibrin
Polydimethylsiloxane
Tumor Biomarkers
Fibrin
Separators
Antibodies
prognosis
biomarkers
Screening
separators

Keywords

  • MEMS
  • MEMS SEC column
  • Miniaturization
  • Separation
  • Size exclusion chromatography

ASJC Scopus subject areas

  • Condensed Matter Physics
  • Electronic, Optical and Magnetic Materials
  • Materials Chemistry

Cite this

Separation of beta-human chorionic gonadotropin from fibrinogen using a MEMS size exclusion chromatography column. / Yang, Yongmo; Choi, Seokheun; Chae, Junseok.

In: Microfluidics and Nanofluidics, Vol. 8, No. 4, 04.2010, p. 477-484.

Research output: Contribution to journalArticle

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N2 - This article reports a MEMS (Micro-Electro-Mechanical-Systems)-based column separator designed for potential integration with portable medical point-of-care testing (POCT) devices. The MEMS column uses size exclusion chromatography (SEC) to pre-separate raw samples by size, and is made of polydimethylsiloxane (PDMS) fabricated on a glass slide. The MEMS SEC column separates 300 ng/mL of beta-human chorionic gonadotropin (β-hCG), a cancer biomarker, from a fibrin-ogen-rich solution (20 μg/mL) in 10 min, showing 2.12 resolution and 0.036 mm plate height through fluorescent detection. Results are further verified by β-hCG and anti-β-hCG antibody conjugate using surface plasmon resonance (SPR). The collected β-hCG-rich eluent at 8 min shows 11 mDeg of angle shift. The fluorescent detection and SPR results demonstrate the complete discrimination of β-hCG from fibrinogen using the MEMS SEC column, and illustrate its viability for integrating a sample preparation stage in POCT devices to assist cancer screening and prognosis.

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