Sensitive detection of cardiac biomarkers using a magnetic microbead immunoassay

Christine F. Woolley, Mark Hayes

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

To achieve improved sensitivity in cardiac biomarker detection, a batch incubation magnetic microbead immunoassay was developed and tested on three separate human protein targets: myoglobin, heart-type fatty acid binding protein, and cardiac troponin I. A sandwich immunoassay was performed in a simple micro-centrifuge tube allowing full dispersal of the solid capture surface during incubations. Following magnetic bead capture and wash steps, samples were analyzed in the presence of a manipulated magnetic field utilizing a modified microscope slide and fluorescent inverted microscope to collect video data files. Analysis of the video data allowed for the quantitation of myoglobin, heart-type fatty acid binding protein and cardiac troponin I to levels of 360 aM, 67 fM, and 42 fM, respectively. Compared to the previous detection limit of 50 pM for myoglobin, this offers a five-fold improvement in sensitivity. This improvement in sensitivity and incorporation of additional markers, along with the small sample volumes required, suggest the potential of this platform for incorporation as a detection method in a total sample analysis device enabling multiplexed detection for the analysis of clinical samples.

Original languageEnglish (US)
Pages (from-to)8632-8639
Number of pages8
JournalAnalytical Methods
Volume7
Issue number20
DOIs
StatePublished - Oct 21 2015

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Myoglobin
Biomarkers
Fatty acids
Fatty Acid-Binding Proteins
Microscopes
Troponin I
Centrifuges
Magnetic fields
Proteins
Carrier Proteins

ASJC Scopus subject areas

  • Analytical Chemistry
  • Engineering(all)
  • Chemical Engineering(all)

Cite this

Sensitive detection of cardiac biomarkers using a magnetic microbead immunoassay. / Woolley, Christine F.; Hayes, Mark.

In: Analytical Methods, Vol. 7, No. 20, 21.10.2015, p. 8632-8639.

Research output: Contribution to journalArticle

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