Sbp1p affects translational repression and decapping in Saccharomyces cerevisiae

Scott P. Segal, Travis Dunckley, Roy Parker

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

The relationship between translation and mRNA turnover is critical to the regulation of gene expression. One major pathway for mRNA turnover occurs by deadenylation, which leads to decapping and subsequent 5′-to-3′ degradation of the body of the mRNA. Prior to mRNA decapping, a transcript exits translation and enters P bodies to become a potential decapping substrate. To understand the transition from translation to decapping, it is important to identify the factors involved in this process. In this work, we identify Sbp1p (formerly known as Ssb1p), an abundant RNA binding protein, as a high-copy-number suppressor of a conditional allele in the decapping enzyme. Sbp1p overexpression restores normal decay rates in decapping-defective strains and increases P-body size and number. In addition, Sbp1p promotes translational repression of mRNA during glucose deprivation. Moreover, P-body formation is reduced in strains lacking Sbp1p. Sbp1p acts in conjunction with Dhh1p, as it is required for translational repression and P-body formation in pat1Δ strains under these conditions. These results identify Sbp1p as a new protein that functions in the transition of mRNAs from translation to an mRNP complex destined for decapping.

Original languageEnglish (US)
Pages (from-to)5120-5130
Number of pages11
JournalMolecular and Cellular Biology
Volume26
Issue number13
DOIs
StatePublished - Jul 1 2006
Externally publishedYes

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Saccharomyces cerevisiae
Protein Biosynthesis
Messenger RNA
RNA-Binding Proteins
RNA Stability
Gene Expression Regulation
Body Size
Alleles
Glucose
Proteins
mRNA decapping enzymes
messenger ribonucleoprotein

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

Cite this

Sbp1p affects translational repression and decapping in Saccharomyces cerevisiae. / Segal, Scott P.; Dunckley, Travis; Parker, Roy.

In: Molecular and Cellular Biology, Vol. 26, No. 13, 01.07.2006, p. 5120-5130.

Research output: Contribution to journalArticle

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