Saccharomyces cerevisiae samples stained with FUN-1 dye can be stored at -20°C for later observation

M. D. Eggleston, Pamela Marshall

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

FUN-1, a fluorescent vital dye, has been observed to form cylindrical intravacuolar structures within the vacuoles of metabolically active yeast cells. FUN-1 staining, which begins as a diffuse pool of fluorescent cytoplasmic stain, uses an unknown endogenous biochemical processing mechanism to compact and form orange-red cylindrical intravacuolar structures within the cell vacuole. In the clinical setting, FUN-1 is primarily used for identification of fungal infection. FUN-1 is utilized in the laboratory to distinguish between metabolically active and dead fungal cells. Although this stain is useful for distinguishing between live and dead fungal dead cells, few studies have utilized this chemical. This lack of use in the scientific community may be due to the requirement that cells are visualized directly after staining. Thus, it would be of interest to be able to stain cells and store them for later use. Our lab examined the longevity of cylindrical intravacuolar structures in two strains of Saccharomyces cerevisiae stained with FUN-1 and stored at -20°C. We found that cylindrical intravacuolar structures could be reliably observed and imaged utilizing differential interference contrast microscopy and fluorescence microscopy for 21 days. We also observed that cells stained with FUN-1 would resume propagation on yeast extract, peptone, dextrose (YPD) plates after being frozen at -20°C for 21 days. These modifications to the published procedure for FUN-1 dye staining should allow for a more prevalent and less time sensitive use of this important biological tool.

Original languageEnglish (US)
Pages (from-to)100-103
Number of pages4
JournalJournal of Microscopy
Volume225
Issue number1
DOIs
StatePublished - Jan 2007

Fingerprint

saccharomyces
Yeast
Saccharomyces cerevisiae
Coloring Agents
Dyes
dyes
Observation
staining
cells
Dextrose
Fluorescence microscopy
yeast
Staining and Labeling
Vacuoles
Microscopic examination
Cells
microscopy
Yeasts
Interference Microscopy
infectious diseases

Keywords

  • FUN-1 dye
  • Saccharomyces cerevisiae
  • Storage
  • Yeast

ASJC Scopus subject areas

  • Instrumentation

Cite this

Saccharomyces cerevisiae samples stained with FUN-1 dye can be stored at -20°C for later observation. / Eggleston, M. D.; Marshall, Pamela.

In: Journal of Microscopy, Vol. 225, No. 1, 01.2007, p. 100-103.

Research output: Contribution to journalArticle

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